Genotoxicity testing of five compounds in three Drosophila short-term somatic assays

Batiste-Alentorn, M.; Xamena, N.; Creus, A.; Marcos, Ricard

doi:10.1016/0165-1218(95)90006-3

Cited by 13 publications

(2 citation statements)

References 26 publications

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“…On account of this and in consideration of all the other advantages offered by rapid somatic assays in D.melanogaster it seems a profitable strategy to screen for genotoxic and recombinagenic activity of compounds in the first instance with this type of assay. On comparing the sensitivities of different somatic assays in Drosophila, such as genetic instabilities in the zeste-white or the white-ivory eye spot test on the one hand and the wing spot test on the other, it became clear that these somatic test systems are not equivalent with respect to the spectra of genotoxic agents they are able to detect (Batiste-Alentorn et al, 1995;Graf and Wu ¨rgler, 1996). The SMART approach appears to be the most potent and, moreover, the wing spot test represents a rapid and inexpensive test method that allows quantitative determination of both the genotoxic and recombinagenic activities of chemical compounds or complex mixtures (Magnusson and Ramel, 1990;Frei et al, 1992;Graf et al, 1992b;Marec and Gelbic, 1994;Guzma ´n-Rinco ´n and Graf, 1995;Frei and Wu ¨rgler, 1996;Graf and Wu ¨rgler, 1996;Rodriguez-Arnaiz et al, 1996; Gonza ´lez-Ce ´sar and Ramos-Morales, 1997; Vogel et al, 1999).…”

Section: Recombinagenicitymentioning

confidence: 99%

Recombinagenic activity of four compounds in the standard and high bioactivation crosses of Drosophila melanogaster in the wing spot test

Spanó

Frei²,

Würgler³

et al. 2001

Mutagenesis

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The wing somatic mutation and recombination test (SMART) using Drosophila melanogaster was employed to determine the recombinagenic and mutagenic activity of four chemicals in an in vivo eukaryotic system. Two different crosses involving the wing cell markers mwh and flr(3) were used: the standard cross and a high bioactivation cross. The high bioactivation cross is characterized by a high constitutive level of cytochromes P450 which leads to an increased sensitivity to a number of promutagens and procarcinogens. Three-day-old larvae derived from both crosses were treated chronically with the oxidizing agent potassium chromate and with the three procarcinogens cyclophosphamide, p-dimethylaminoazobenzene and 9,10-dimethylanthracene. From both crosses two types of progeny were obtained: marker-heterozygous and balancer-heterozygous. The wings of both genotypes were analysed for the occurrence of single and twin spots expressing the mwh and/or flr(3) mutant phenotypes. In the marker-heterozygous genotype the spots can be due either to mitotic recombination or to mutation. In contrast, in the balancer-heterozygous genotype only mutational events lead to spot formation, all recombination events being eliminated. The oxidizing agent potassium chromate was equally and highly genotoxic in both crosses. Surprisingly, the promutagen cyclophosphamide also showed equal genotoxicity in both crosses, whereas p-dimethylaminoazobenzene was negative in the standard cross, but clearly genotoxic in the high bioactivation cross. 9,10-Dimethylanthracene showed a rather weak genotoxicity in the high bioactivation cross. Analyses of the dose-response relationships for mwh clones recorded in the two wing genotypes demonstrated that all four compounds are recombinagenic. The fraction of all genotoxic events which are due to mitotic recombination ranged from 83% (9,10-dimethylanthracene) to 99% (p-dimethylaminoazobenzene). These results demonstrate that the wing spot test in Drosophila is most suited to the detection of recombinagenic activity of genotoxic chemicals.

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Section: Recombinagenicitymentioning

confidence: 99%

Recombinagenic activity of four compounds in the standard and high bioactivation crosses of Drosophila melanogaster in the wing spot test

Spanó

Frei²,

Würgler³

et al. 2001

Mutagenesis

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“…In the L5178Y mouse lymphoma mutagenicity assay (Dearfield et al, 1993), 2-and 3-chloropyridine produced small increases in mutants. The mutagenicity of 2-chloropyridine was greater with bioactivation and it was also mutagenic in Drosophila (Batiste-Alentorn et al, 1995). Pyridine itself produced liver tumors in male and female B6C3F1 mice, while findings were equivocal in rats (National Toxicology Program, 1996).…”

Section: Human Effects Of Rodent Nasal Cytotoxinsmentioning

confidence: 99%

Nasal Cytotoxic and Carcinogenic Activities of Systemically Distributed Organic Chemicals

2006

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Toxicity and carcinogenicity in the mucosa of the nasal passages in rodents has been produced by a variety of organic chemicals which are systemically distributed. In this review, 14 such chemicals or classes were identified that produced rodent nasal cytotoxicity, but not carcinogenicity, and 11 were identified that produced nasal carcinogenicity. Most chemicals that affect the nasal mucosa were either concentrated in that tissue or readily activated there, or both. All chemicals with effects in the nasal mucosa that were DNA-reactive, were also carcinogenic, if adequately tested. None of the rodent nasal cytotoxins has been identified as a human systemic nasal toxin. This may reflect the lesser biotransformation activity of human nasal mucosa compared to rodent and the much lower levels of human exposures. None of the rodent carcinogens lacking DNA reactivity has been identified as a nasal carcinogen or other cancer hazard to humans. Some DNA-reactive rodent carcinogens that affect the nasal mucosa, as well as other tissues, have been associated with cancer at various sites in humans, but not the nasal cavity. Thus, findings in only the rodent nasal mucosa do not necessarily predict either a toxic or carcinogenic hazard to that tissue in humans.

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The somaticwhite-ivory eye spot test does not detect the same spectrum of genotoxic events as the wing somatic mutation and recombination test inDrosophila melanogaster

Gräf

Würgler

1996

Environ. Mol. Mutagen.

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Genotoxicity testing of five compounds in three Drosophila short-term somatic assays

Cited by 13 publications

References 26 publications

Recombinagenic activity of four compounds in the standard and high bioactivation crosses of Drosophila melanogaster in the wing spot test

Recombinagenic activity of four compounds in the standard and high bioactivation crosses of Drosophila melanogaster in the wing spot test

Nasal Cytotoxic and Carcinogenic Activities of Systemically Distributed Organic Chemicals

The somaticwhite-ivory eye spot test does not detect the same spectrum of genotoxic events as the wing somatic mutation and recombination test inDrosophila melanogaster

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