Genotoxicological Evaluation of NUTRALYS Pea Protein Isolate

Abstract

NUTRALYS Pea Protein Isolate, a protein supplement, is a high-quality source of protein which is primarily emulsifying functional protein. We evaluated the genotoxic potential of NUTRALYS isolated from dry yellow pea, using three established genotoxicity tests (AMES test in vitro chromosomal aberration test, and in vivo micronucleus test) employing OECD guidelines under GLP conditions. In the bacterial reverse mutation test, NUTRALYS did not show positive responses in strains detecting point and frame shift mutations. In the chromosomal aberration test, NUTRALYS did not induce chromosome aberrations in the presence and absence of metabolic activation. In the bone marrow micronucleus test, NUTRALYS did not induce significant increases of micronucleated immature (polychromatic) erythrocytes in bone marrow of test animals.

Health Benefits of Pea Protein Isolate: A Comparative Review

“…Lastly, an in vivo mice bone marrow micronucleus assay was used and once again the PPI did not induce significant increases of micronucleated immature erythrocytes in the bone marrow [12]. Thus, it was concluded that NUTRALYS PPI is nonmutagenic and nongenotoxic from the 3 first line tests used [12]. Further research could be conducted based off of Auoatif et al's study; however, current research efforts are seemingly aimed at other properties of PPI.…”

“…In this study, three first barrier trials were used. Firstly, the PPI did not show a positive result of identifying point and frameshift mutations in the Ames bacterial reverse mutation test [12]. The second test was an in vitro chromosomal aberration test and the PPI again did not induce chromosomal aberrations in the presence or absence of metabolic activation in human lymphocytes [12].…”

“…Genotoxicity is the process that causes damage to genetic material, which is why its regulation is crucial for new products entering the food market for direct human consumption [11]. Aouatif et al evaluated the safety of the pea protein isolates, specifically from NUTRALYS and its genotoxic potential [12]. In this study, three first barrier trials were used.…”

“…Firstly, the PPI did not show a positive result of identifying point and frameshift mutations in the Ames bacterial reverse mutation test [12]. The second test was an in vitro chromosomal aberration test and the PPI again did not induce chromosomal aberrations in the presence or absence of metabolic activation in human lymphocytes [12]. Lastly, an in vivo mice bone marrow micronucleus assay was used and once again the PPI did not induce significant increases of micronucleated immature erythrocytes in the bone marrow [12].…”

“…The second test was an in vitro chromosomal aberration test and the PPI again did not induce chromosomal aberrations in the presence or absence of metabolic activation in human lymphocytes [12]. Lastly, an in vivo mice bone marrow micronucleus assay was used and once again the PPI did not induce significant increases of micronucleated immature erythrocytes in the bone marrow [12]. Thus, it was concluded that NUTRALYS PPI is nonmutagenic and nongenotoxic from the 3 first line tests used [12].…”