Genotype analysis of Candida albicans isolates obtained from different body locations of patients with superficial candidiasis using PCRs targeting 25S rDNA and ALT repeat sequences of the RPS

Hattori, Hisao; Iwata, Takako; Nakagawa, Yoshiyuki; Kawamoto, Fumihiko; Tomita, Yasushi; Kikuchi, Akihiko; Kanbe, Toshio

doi:10.1016/j.jdermsci.2005.12.003

Cited by 25 publications

(33 citation statements)

References 30 publications

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“…One approximation for this study was to interpret the blood isolates as surrogate markers for baseline colonization and to compare this to subsequent oral colonization. Genotyping revealed a 90% genetic correlation for species acquired from both sites, supporting the general concept that the invasive species was also a colonizing agent, as previously shown (15,(37)(38)(39)(40). In addition, the MIC data for paired blood and oral isolates mirrored those for the overall isolate collection for both C. albicans and C. glabrata.…”

Section: Discussionsupporting

confidence: 79%

Posttreatment Antifungal Resistance among Colonizing Candida Isolates in Candidemia Patients: Results from a Systematic Multicenter Study

Jensen

Johansen

Søes

et al. 2016

Antimicrob Agents Chemother

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The prevalence of intrinsic and acquired resistance among colonizing Candida isolates from patients after candidemia was investigated systematically in a 1-year nationwide study. Patients were treated at the discretion of the treating physician. Oral swabs were obtained after treatment. Species distributions and MIC data were investigated for blood and posttreatment oral isolates from patients exposed to either azoles or echinocandins for <7 or >7 days. Species identification was confirmed using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and internal transcribed spacer (ITS) sequencing, susceptibility was examined by EUCAST EDef 7.2 methodology, echinocandin resistance was examined by FKS sequencing, and genetic relatedness was examined by multilocus sequence typing (MLST). One hundred ninety-three episodes provided 205 blood and 220 oral isolates. MLST analysis demonstrated a genetic relationship for 90% of all paired blood and oral isolates. Patients exposed to azoles for >7 days (n ‫؍‬ 93) had a significantly larger proportion of species intrinsically less susceptible to azoles (particularly Candida glabrata) among oral isolates than among initial blood isolates (36.6% versus 12.9%; P < 0.001). A similar shift toward species less susceptible to echinocandins among 85 patients exposed to echinocandins for >7 days was not observed (4.8% of oral isolates versus 3.2% of blood isolates; P > 0.5). Acquired resistance in Candida albicans was rare (<5%). However, acquired resistance to fluconazole (29.4%; P < 0.05) and anidulafungin (21.6%; P < 0.05) was common in C. glabrata isolates from patients exposed to either azoles or echinocandins. Our findings suggest that the colonizing mucosal microbiota may be an unrecognized reservoir of resistant Candida species, especially C. glabrata, following treatment for candidemia. The resistance rates were high, raising concern in general for patients exposed to antifungal drugs.

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Section: Discussionsupporting

confidence: 79%

Posttreatment Antifungal Resistance among Colonizing Candida Isolates in Candidemia Patients: Results from a Systematic Multicenter Study

Jensen

Johansen

Søes

et al. 2016

Antimicrob Agents Chemother

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“…PCR targeting 25S rDNA, which has frequently been used for genotype analysis of C. albicans, allows, on the basis of the length of the amplified PCR product, C. albicans isolates to be grouped into three main genotypes A, B, and C (Tamura et al, 2001;Hattori et al, 2006;McCullough et al, 1999;Iwata et al, 2006;Millar et al, 2002). In our study, the most prevalent C. albicans genotype among all isolates on the basis of amplification of 25S rDNA was genotype C (55%), followed by genotype A (32.4%), and B (12.6%).…”

Section: Discussionmentioning

confidence: 99%

Genotyping and antifungal susceptibility of Candida albicans strains from patients with vulvovaginal and cutaneous candidiasis in Palestine

Ali‐Shtayeh¹,

Jamous²,

Alothman³

et al. 2015

Afr. J. Microbiol. Res.

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“…Isolates in different samples from the same patient showed exactly the same CAI SSCP pattern (e.g. isolates from patients CY61, CY65, CY66, CY69, CY141, CY119, CY123, CY68 and CY154 in Table 2), indicating that the infection was mainly endogenous [14,31]. …”

Section: Discussionmentioning

confidence: 99%

Surveillance of the prevalence, antibiotic susceptibility, and genotypic characterization of invasive candidiasis in a teaching hospital in China between 2006 to 2011

Lin

Cao

et al. 2013

BMC Infect Dis

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BackgroundInvasive candidiasis is an important nosocomial infection associated with high mortality among immunosuppressive or critically ill patients. We described the incidence of invasive candidiasis in our hospital over 6 years and showed the antifungal susceptibility and genotypes of the isolated yeast.MethodThe yeast species were isolated on CHROMagar Candida medium and identified using an yeast identification card, followed by analysis of the D1/D2 domain of 26S rDNA. The susceptibilities of the isolates to flucytosine, amphotericin B, fluconazole, itraconazole, and voriconazole were tested using the ATB FUNGUS 3 system, and that to caspofungin was tested using E-test strips. C. albicans was genotyped using single-strand conformation polymorphism of CAI (Candida albicans I) microsatellite DNA combined with GeneScan data.ResultsFrom January 2006 to December 2011, a total of 259 isolates of invasive Candida spp. were obtained from 253 patients, among them 6 patients had multiple positive samples. Ninety-one stains were from blood and 168 from sterile fluids, accounting for 6.07% of all pathogens isolated in our hospital. Most of these strains were C. albicans (41.29% in blood/59.06% in sterile body fluids), followed by C. tropicalis (18.06%/25.72%), C. parapsilosis (17.42%/5.43%), C. glabrata (11.61%/3.99%) and other Candida spp. (11.61%/5.80%). Most Candida spp. were isolated from the ICU. The new species-specific CLSI candida MIC breakpoints were applied to these date. Resistance to fluconazole occurred in 6.6% of C. albicans isolates, 10.6% of C. tropicalis isolates and 15.0% of C. glabrata isolates. For the 136 C. albicans isolates, 54 CAI patterns were recognized. The C. albicans strains from blood or sterile body fluids showed no predominant CAI genotypes. C. albicans isolates from different samples from the same patient had the same genotype.ConclusionsInvasive candidiasis has been commonly encountered in our hospital in the past 6 years, with increasing frequency of non-C. albicans. Resistance to fluconazole was highly predictive of resistance to voriconazole. CAI SSCP genotyping showed that all C. albicans strains were polymorphic. Invasive candidiasis were commonly endogenous infection.

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Genotype analysis of Candida albicans isolates obtained from different body locations of patients with superficial candidiasis using PCRs targeting 25S rDNA and ALT repeat sequences of the RPS

Cited by 25 publications

References 30 publications

Posttreatment Antifungal Resistance among Colonizing Candida Isolates in Candidemia Patients: Results from a Systematic Multicenter Study

Posttreatment Antifungal Resistance among Colonizing Candida Isolates in Candidemia Patients: Results from a Systematic Multicenter Study

Genotyping and antifungal susceptibility of Candida albicans strains from patients with vulvovaginal and cutaneous candidiasis in Palestine

Surveillance of the prevalence, antibiotic susceptibility, and genotypic characterization of invasive candidiasis in a teaching hospital in China between 2006 to 2011

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