Genotype-dependent gene expression profile of the antioxidant defense system (ADS) in the liver of a GH-transgenic zebrafish model

Rosa, Carlos Eduardo da; Figueiredo, Márcio Azevedo; Lanes, Carlos Frederico Ceccon; Almeida, Daniela Volcan; Marins, Luís Fernando

doi:10.1007/s11248-010-9395-4

Cited by 11 publications

(4 citation statements)

References 14 publications

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“…According to the Minimum Information for Publication of Quantitative Real Time PCR Experiments (MIQE) 46 , a RG needs to be validated and established for each species and for different physiological conditions. Our results show that although the literature has already demonstrated alterations in the metabolism of the F0104 lineage 19 , 21 , 29 – 35 , 38 , 47 , 48 , these animals, in comparison to non-transgenic animals, did not suffer notable variations in the expression of most of the analyzed genes.…”

Section: Discussionsupporting

confidence: 45%

Evaluation of qPCR reference genes in GH-overexpressing transgenic zebrafish (Danio rerio)

Rassier

Silveira

Remião

et al. 2020

Sci Rep

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Reference genes (RGs) must have a stable expression in tissues in all experimental conditions to normalize real-time quantitative reverse transcription PCR (qRT-PCR) data. F0104 is a highly studied lineage of zebrafish developed to overexpress the growth hormone (GH). It is assumed that the transgenic process may influence the expression levels of commonly used RGs. The objective of the present study was to make a comprehensive analysis of stability of canditade RGs actb1, actb2, b2m, eif2s2, eef1a1, gapdh, rplp2, rpl7, rpl13α, tuba1, and rps18, in gh -transgenic and non-transgenic zebrafish. Liver, brain, intestine and muscle samples from both groups had qRT-PCR results analyzed by dCt, geNorm, NormFinder, BestKeeper, and RefFinder softwares. Consensus analyses among software concluded that rpl13α , rpl7 , and eef1a1 are the most stable genes for zebrafish, considering the studied groups and tissues. Gapdh , rps18 , and tuba1 suffered variations in stability among different tissues of both groups, and so, they were listed as the genes with lowest stability. Results from an average pairwise variations test indicated that the use of two RGs would generate reliable results for gene expression analysis in the studied tissues. We conclude that genes that are commonly used in mammals for qRT-PCR assays have low stability in both non-transgenic and gh-transgenic zebrafish reinforcing the importance of using species-specific RGs.

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Section: Discussionsupporting

confidence: 45%

Evaluation of qPCR reference genes in GH-overexpressing transgenic zebrafish (Danio rerio)

Rassier

Silveira

Remião

et al. 2020

Sci Rep

View full text Add to dashboard Cite

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“…It was shown that gh -transgenic zebrafish grew faster in a shorter period than their non-transgenic full sibling controls ( Figueiredo et al, 2007 ; Rosa et al, 2010 ; Silva et al, 2015 ) and presented enhanced muscle hypertrophy when compared to non-transgenic fish ( Kuradomi et al, 2011 ). GH overexpression also impacts oxidative metabolism ( Rosa et al, 2008 , 2011 ), aging ( Rosa et al, 2010 ), reproduction ( Figueiredo et al, 2013 ), ionic balance ( Almeida et al, 2013 ), immune system ( Batista et al, 2014 ), and appetite behavior ( Dalmolin et al, 2015 ) in this gh -transgenic zebrafish line. Collectively, these findings provide evidence that the excess of circulating GH implies mandatory growth even in unfavorable conditions, resulting in higher metabolic demand.…”

Section: Introductionmentioning

confidence: 96%

Growth Hormone Overexpression Induces Hyperphagia and Intestinal Morphophysiological Adaptations to Improve Nutrient Uptake in Zebrafish

et al. 2021

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Graphical AbstractGH overexpression increases the intestinal mass and absorptive surface area both through the direct effects of GH transgenics (up-regulating the expression of its cognate receptor and insulin-like growth factor, igf1a) and through indirect effects through an increase in the amount of food consumed. In addition, hyperphagia provides a greater amount of nutrients in the intestinal lumen and stimulates the synthesis of di- and tri-peptide transporters, which are the main route of absorption of dietary products from protein degradation.

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“…Cataracts are a well-known effect of oxidative stress to the lens [ 7 ]. Furthermore, growth hormone transgenic fish that have a high growth rate are known to have elevated oxidative stress [ 8 , 9 , 10 , 11 ]. These studies indicate a possible involvement of redox regulation.…”

Section: Introductionmentioning

confidence: 99%

Impact of Antioxidant Feed and Growth Manipulation on the Redox Regulation of Atlantic Salmon Smolts

et al. 2022

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Accumulating evidence indicates a close relationship between oxidative stress and growth rate in fish. However, the underlying mechanisms of this relationship remain unclear. This study evaluated the combined effect of dietary antioxidants and growth hormone (GH) on the liver and the muscle redox status of Atlantic salmon. There were two sequential experimental phases (EP) termed EP1 and EP2, each lasting for 6 weeks. In EP1, Atlantic salmon were fed either low-(L, 230 mg/kg ascorbic acid (Asc), 120 mg/kg α-tocopherol (α-TOH)), or high-(H, 380 mg/kg Asc, 210 mg/kg α-TOH)vitamin diets. The vitamins were supplemented as stable forms and the feeding was continued in EP2. In EP2, half of the fish were implanted with 3 μL per g body weight of recombinant bovine GH (Posilac®, 1 mg rbGH g BW-1) suspended in sesame oil, while the other half were held in different tanks and sham-implanted with similar volumes of the sesame oil vehicle. Here, we show that increasing high levels of vitamin C and E (diet H) increased their content in muscle and liver during EP1. GH implantation decreased vitamin C and E levels in both liver and muscle but increased malondialdehyde (MDA) levels only in the liver. GH also affected many genes and pathways of antioxidant enzymes and the redox balance. Among the most consistent were the upregulation of genes coding for the NADPH oxidase family (NOXs) and downregulation of the oxidative stress response transcription factor, nuclear factor-erythroid 2-related factor 2 (nrf2), and its downstream target genes in the liver. We verified that GH increases the growth rate until the end of the trail and induces an oxidative effect in the liver and muscle of Atlantic salmon. Dietary antioxidants do lower oxidative stress but have no effect on the growth rate. The present study is intended as a starting point to understand the potential interactions between growth and redox signaling in fish.

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Genotype-dependent gene expression profile of the antioxidant defense system (ADS) in the liver of a GH-transgenic zebrafish model

Cited by 11 publications

References 14 publications

Evaluation of qPCR reference genes in GH-overexpressing transgenic zebrafish (Danio rerio)

Evaluation of qPCR reference genes in GH-overexpressing transgenic zebrafish (Danio rerio)

Growth Hormone Overexpression Induces Hyperphagia and Intestinal Morphophysiological Adaptations to Improve Nutrient Uptake in Zebrafish

Impact of Antioxidant Feed and Growth Manipulation on the Redox Regulation of Atlantic Salmon Smolts

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