Genotype frequency of human neutrophil antigen‐3 polymorphisms in the Yi, Han, and Tibetan populations of China

Abstract

Background Human neutrophil antigen-3 (HNA-3) alloantibodies can cause fatal transfusion-related acute lung injury (TRALI). Most frequencies of SLC44A2 alleles encoding the HNA-3a/b antigens have been established in Han individuals by PCR-SSP. We sequenced a SLC44A2 gene fragment and determined its allele frequencies in 3 ethnicities of China. Study design and methods Genomic DNA was extracted from 448 samples of 100 blood donors of Yi ethnicity in Xichang, Liangshan; 248 Han in Nanjing, Jiangsu; and 100 Tibetan in Lhasa, Tibet. A PCR-SSP was applied to determine the phase of 2 SNPs; SLC44A2 haplotypes were constructed. Results In the 567 nucleotides of the SLC44A2 gene covered by our sequencing approach in Han individuals, we detected the known 331-44G>A (rs12972963) and 461G>A (rs2288904) polymorphisms. In the 243 nucleotides sequenced in Yi and Tibetan populations, we detected the known 461G>A and 503-15T>C (rs1560711) polymorphisms. A PCR-SSP for the common HNA-3a/b SNP was 100% concordant. The frequencies of the HNA-3a allele were 0.58, 0.66 and 0.69 in Yi, Han (Nanjing) and Tibetan, respectively (0.42, 0.34 and 0.31 for HNA-3b). Conclusions The Yi population of China had the highest frequency of blood donors at risk of harboring anti-HNA-3a compared to any population studied so far. We confirmed that the underlying SLC44A2*2 allele is more common in China than in any European or African populations.

Assessment of HNA alloimmunisation risk in Northeastern Thais, Burmese and Karen

“…Therefore, Northeastern Thais and Burmese may have a higher probability than Karen, potentially forming anti‐HNA‐3a and linking to TRALI. However, the prevalence of the HNA‐3b allele was found to be 0·170–0·239 in Caucasians and 0·026–0·070 in Africans (Chen et al ., ). An estimation of the possible risk of HNA‐3a alloimmunisation in Northeastern Thais, Burmese and Karen showed values of 0·0493, 0·0608 and 0·0196, respectively.…”

A study of human neutrophil antigen genotype frequencies in Hong Kong

“…However, the newly reported HNA‐3a with 457C > T was not covered by these primer sets. Finally, more recent studies have shown very extensive polymorphisms of the SLC44A2 gene encoding HNA‐3, and the geographical variation of these new alleles and their serological significance and clinical impact remain to be determined (Chen et al, ,b).…”

Full‐length nucleotide sequences of 30 common SLC44A2 alleles encoding human neutrophil antigen‐3

“…We developed a sensitive and specific screening assay to genotype the SLC44A2 gene in a high‐throughput setting. Our decision tree can be applied to any population . A computational approach to predict HNA‐3 antigens from next generation sequencing or WGS data will require a method for detecting carriers of low‐prevalence alleles.…”

“…The frequency of the SNP indicative of the SLC44A2*1 and SLC44A2*2 alleles is known for Caucasian, African American, Han Chinese, Japanese, Tunisian, Brazilian, Thai, and other populations . Chu and colleagues applied an algorithmic approach to predict HNA antigens from whole genome sequencing (WGS) data.…”