Genotype I African swine fever viruses emerged in domestic pigs in China and caused chronic infection

Sun, E; Huang, Lianyu; Zhang, Xianfeng; Zhang, Jiwen; Shen, Daleng; Zhang, Zhenjiang; Wang, Zilong; Hong, Hao; Wang, Wenqing; Huangfu, Haoyue; Wang, Wan; Li, Fang; Liu, Renqiang; Sun, Jianhong; Tian, Zhen; Xia, Wei; Guan, Yuntao; He, Xijun; Zhu, Yuanmao; Zhao, Dongming; Bu, Zhigao

doi:10.1080/22221751.2021.1999779

Cited by 173 publications

(188 citation statements)

References 41 publications

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“…When testing swine anticoagulant blood and cloacal swabs with lower virus levels, the positivity rates of the A137R gene-based real-time PCR was higher than OIE-recommended TaqMan PCR. Recently, genotype I ASFV has been reported in domestic pigs in China ( 27 ). The A137R primers used in the developed SYBR Green-based real-time PCR targeting the A137R gene of ASFV are highly conserved across all known ASFV genotypes, including genotype I, making it possible to detect both genotype I and II ASFV co-circulating in China at the moment.…”

Section: Discussionmentioning

confidence: 99%

Development of Real-Time PCR Based on A137R Gene for the Detection of African Swine Fever Virus

Yin

Geng

et al. 2021

Front. Vet. Sci.

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African swine fever virus (ASFV) can infect domestic pigs and wild boars and causes huge economic losses in global swine industry. Therefore, early diagnosis of ASFV is important for the control and eradication of African swine fever (ASF). In this study, a SYBR Green-based real-time polymerase chain reaction (PCR) assay targeting the viral encoded A137R gene was established for the detection of ASFV infection. For the evaluation of the established real-time PCR, 34 clinical samples were assessed by both the A137R gene-based real-time PCR and OIE-recommended TaqMan PCR. The results showed that 85.29% (29/34) were detected by A137R gene-based real-time PCR, but only 79.41% (27/34) positive using OIE-recommended TaqMan PCR. Moreover, no cross-reaction with other common swine pathogens was found in the A137R gene-based real-time PCR. These results demonstrated that the established real-time PCR assay in this study showed better performance than the OIE-recommended method in detecting ASFV from clinical samples, which could be applied for control and eradication programs of ASF.

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Section: Discussionmentioning

confidence: 99%

Development of Real-Time PCR Based on A137R Gene for the Detection of African Swine Fever Virus

Yin

Geng

et al. 2021

Front. Vet. Sci.

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“…The situation may be aggravated by using non-authorised vaccines in attempts to stop the disease from spreading. These practices have led to the circulation of low-virulence strains in China and have facilitated the presence of animals with chronic forms and persistent infections [22,23]. Far from helping, this situation is posing a great obstacle to the early detection of the disease and its subsequent control and eradication [24].…”

Section: Discussionmentioning

confidence: 99%

Quantitative Risk Assessment of African Swine Fever Introduction into Spain by Legal Import of Live Pigs

et al. 2022

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African swine fever (ASF) is a devastating infectious disease of pigs that is threatening the global swine industry at present. The current spread of ASF in Europe and its recent incursion into Germany pose a serious risk to Spain, one of the world’s leading pig producers. A quantitative stochastic risk assessment model was developed to estimate the probability of ASF introduction into Spain via the legal import of live pigs. The results suggest a low annual probability of ASF introduction into Spain (1.07 × 10−4), the highest risk being concentrated in Central European countries (Germany, the Netherlands, Belgium, and Luxembourg) during the months of April and February. The methods and results presented herein could contribute to improving prevention and control strategies and, ultimately, would help reduce the risk of ASF introduction into Spain.

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“…Currently, 24 genotypes have been identified by sequencing the major capsid protein (p72) gene (B646L); in particular, genotype I and genotype II have been reported outside Africa, with the latter being the cause of the ongoing pandemic [233,240]. Nevertheless, genotype I was recently described in China [241].…”

Section: African Swine Fever Virusmentioning

confidence: 99%

The Swine IFN System in Viral Infections: Major Advances and Translational Prospects

Razzuoli

Armando²,

Paolis

et al. 2022

Pathogens

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Interferons (IFNs) are a family of cytokines that play a pivotal role in orchestrating the innate immune response during viral infections, thus representing the first line of defense in the host. After binding to their respective receptors, they are able to elicit a plethora of biological activities, by initiating signaling cascades which lead to the transcription of genes involved in antiviral, anti-inflammatory, immunomodulatory and antitumoral effector mechanisms. In hindsight, it is not surprising that viruses have evolved multiple IFN escape strategies toward efficient replication in the host. Hence, in order to achieve insight into preventive and treatment strategies, it is essential to explore the mechanisms underlying the IFN response to viral infections and the constraints thereof. Accordingly, this review is focused on three RNA and three DNA viruses of major importance in the swine farming sector, aiming to provide essential data as to how the IFN system modulates the antiviral immune response, and is affected by diverse, virus-driven, immune escape mechanisms.

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Genotype I African swine fever viruses emerged in domestic pigs in China and caused chronic infection

Cited by 173 publications

References 41 publications

Development of Real-Time PCR Based on A137R Gene for the Detection of African Swine Fever Virus

Development of Real-Time PCR Based on A137R Gene for the Detection of African Swine Fever Virus

Quantitative Risk Assessment of African Swine Fever Introduction into Spain by Legal Import of Live Pigs

The Swine IFN System in Viral Infections: Major Advances and Translational Prospects

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