Genotype-independent transformation of lettuce using<i>Agrobacterium tumefaciens</i>

Curtis, Ian S.; Power, J. B.; Blackhall, N. W.; Laat, A.M.M. de; Davey, M. R.

doi:10.1093/jxb/45.10.1441

Cited by 87 publications

(55 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Seven-day-old cotyledon explants were transformed by cocultivation with an LBA4404 Agrobacterium tumefaciens strain harboring the binary vector pVDH282 (a pBIN19 derivative), which contained the Arabidopsis KNAT1 cDNA under the control of the pea plastocyanin promoter PetE (Pwee and Gray, 1993;Helliwell et al, 1997). The transformation procedure was as described by Curtis et al (1994). Transgenic plants were selected on 50 mg L Ϫ1 kanamycin-selective medium.…”

Section: Plant Growth Conditions and Transformationmentioning

confidence: 99%

Overexpression of KNAT1 in Lettuce Shifts Leaf Determinate Growth to a Shoot-Like Indeterminate Growth Associated with an Accumulation of Isopentenyl-Type Cytokinins

Frugis¹,

Giannino²,

Mele³

et al. 2001

Plant Physiology

119

View full text Add to dashboard Cite

Leaves are specialized organs characterized by defined developmental destiny and determinate growth. The overexpression ofKnotted1-like homeobox genes in different species has been shown to alter leaf shape and development, but a definite role for this class of genes remains to be established. Transgenics that overexpress Knotted1-like genes present some traits that are characteristic of altered cytokinin physiology. Here we show that lettuce (Lactuca sativa) leaves that overexpressKNAT1, an Arabidopsis kn1-like gene, acquire characteristics of indeterminate growth typical of the shoot and that this cell fate change is associated with the accumulation of specific types of cytokinins. The possibility that the phenotypic effects of KNAT1 overexpression may arise primarily from the modulation of local ratios of different cytokinins is discussed.

show abstract

Section: Plant Growth Conditions and Transformationmentioning

confidence: 99%

Overexpression of KNAT1 in Lettuce Shifts Leaf Determinate Growth to a Shoot-Like Indeterminate Growth Associated with an Accumulation of Isopentenyl-Type Cytokinins

Frugis¹,

Giannino²,

Mele³

et al. 2001

Plant Physiology

119

View full text Add to dashboard Cite

show abstract

“…Otras aplicaciones biotecnológicas incluyen el desarrollo de granos con cantidades elevadas de vitamina E, la cual estimula el sistema inmunológico, frutas con mayor contenido de vitamina C. También por medio de la ingeniería genética, ha sido posible desarrollar vegetales que expresan antígenos, conocidos como vacunas comestibles (7).…”

Section: Biotechnology Applied To Food and Human Healthunclassified

“…Para la producción de vacunas comestibles, se utiliza como vector la Agrobacterium tumefaciens. El vector libera en las células vegetales el gen que codifica el antígeno del virus o de la bacteria patogénica, y en consecuencia ocurre una respuesta inmune en el individuo al ingerir el vegetal (7). Al contrario del método convencional de producción de vacunas, este nuevo método no necesita de purificación, pues la acción de las proteínas antigénicas es directa.…”

Section: B) Mayor Calidad Y Cantidad De Proteínas En Vegetalesunclassified

Biotecnologia aplicada a la alimentación y salud humana

Silva

Crispim

et al. 2012

Rev. chil. nutr.

View full text Add to dashboard Cite

“…In the case of the ornamental, Kalanchoë laciniata, a period of 7 days can greatly increase the number of transformed shoots compared to shorter co-culture times [26]. Other factors may be used to improve leaf disc transformation for a specific plant species, including the use of a nurse culture, the inclusion of a supervirulent plasmid within the Agrobacterium [27], the presence of vir-inducing phenolic compounds in the inoculation medium [28] and optimizing the pH of the cocultivation medium [29]. Many dicotyledonous plants can be transformed by floating explants on a suspension of Agrobacterium, but each individual plant species may require specific modifications to achieve optimal transformation efficiency.…”

Section: Inoculation Proceduresmentioning

confidence: 99%