Genotype Influence on Somatic Embryogenesis in Grapevine Anther and Leaf Cultures

Gribaudo, Ivana; Vallania, R.; Franks, Tricia K.; Miaja, M. L.; Thomas, Mark R.

doi:10.17660/actahortic.2000.528.60

Cited by 3 publications

(3 citation statements)

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“…When culturing immature leaf lobes on P-CIM, higher POES was obtained in TME 8, TME 282, TME 203, TME 127, TME 5, TME 12, TMS 93/0665(3·), TMS 91/02327 than for the other genotypes tested (Table 1). Our results on the genotypic variation in somatic embryo formation agreed with previous reports on cassava cultivars originating from South America (Szabados et al, 1987), Africa (Taylor et al, 1993;Konan et al, 1994), Asia (Sofiari et al, 1997), and in several other plant species (Chengalrayan et al, 1998;Gribaudo et al, 2000). P-CIM is a promising medium for somatic embryo induction of a range of cassava genotypes.…”

Section: Resultssupporting

confidence: 92%

Regeneration of a wide range of African cassava genotypes via shoot organogenesis from cotyledons of maturing somatic embryos and conformity of the field-established regenerants

Hankoua

Ng²,

Fawole

et al. 2005

Plant Cell Tiss Organ Cult

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Genotypic differences in the ability of immature leaf lobes and apical shoot meristems of cassava to form primary somatic embryos in P-CIM were observed ( p £ 0.05). The mean number of apical meristems forming primary organized embryogenic structures when cultured in embryo induction medium supplemented with picloram (P-CIM) had greatest variability between genotypes (C.V. ¼ 22.70%). Maturation frequencies of primary embryos were genotype-dependent and ranged from 17 to 100%. Secondary embryo formation was also genotype-dependent and their maturation frequencies varied from 48 to 100%. Cyclic somatic embryogenesis was successfully established and maintained in 11 genotypes in P-CIM. All genotypes underwent organogenesis with significant genotypic variation ( p £ 0.05), and organogenic potential ranging from 5.4 to 76.8%. The number of somatic cotyledons forming multiple shoot buds or more than 10 shoot buds per cluster had the greatest variability between genotypes (C.V. ¼ 36.96%) as compared with the overall embryogenic potential. Shoot regeneration ability was neither related to primary embryogenic potential nor to explant type for primary embryo induction. Plantlet regeneration per responding explant ranged from 0.1 to 12. Regenerants established in the field at the frequency ranging from 60 to 100%. DNA content of regenerants was homogeneous and similar to that of mother plants and ploidy level was unchanged (2n ¼ 36). The potential benefits of a systematic tissue culture approach for screening agronomically superior genotypes for regeneration capability and its usefulness in selecting those suited for transgenic programs are discussed.Abbreviations: BAP -benzyl amino purine; DAPI -4,6-diamidino-2-phenylindole; GLM -general linear model; IBA -indolebutyric acid; picloram -3,5,6-trichloro-4-aminopicolinicacid; SSA -sub-Saharan Africa

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Section: Resultssupporting

confidence: 92%

Regeneration of a wide range of African cassava genotypes via shoot organogenesis from cotyledons of maturing somatic embryos and conformity of the field-established regenerants

Hankoua

Ng²,

Fawole

et al. 2005

Plant Cell Tiss Organ Cult

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“…Concerning anther culture, these correlations had been tested for several species, grapevine included, with parameters such as the number of d before anthesis, the anther color and size, the anther and floral bud lengths, or the microsporogenesis developmental stage being used to select anthers at the most appropriated stage for embryogenesis induction (Mauro et al 1986;Gray and Mortensen 1987;Stamp and Meredith 1988;Newton and Goussard 1990;Harst-Langenbucher and Alleweldt 1993;Mozsár and Süle 1994;Perl et al 1995;Faure et al 1996a, b;Nakano et al 1997;Salunkhe et al 1999;Gribaudo et al 2000;Martinelli et al 2000Martinelli et al , 2001Gribaudo et al 2004;Cardoso et al 2009). …”

Section: Introductionmentioning

confidence: 99%

“…For ovary culture initiation, authors usually cite the number of d before flower anthesis or the anther color (Gray and Mortensen 1987;Mozsár and Süle 1994;Perl et al 1995;Faure et al 1996a, b;Nakano et al 1997;Gribaudo et al 2000;Martinelli et al 2000Martinelli et al , 2001, the floral bud length (Salunkhe et al 1999), the anther length (Mauro et al 1986;Newton and Goussard 1990;HarstLangenbucher and Alleweldt 1993;Perl et al 1995), and the floral developmental stage (Gambino et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Use of morphometric parameters for tracking ovule and microspore evolution in grapevine (Vitis vinifera L., cv. “Aragonez”) and evaluation of their potential to improve in vitro somatic embryogenesis efficiency from gametophyte tissues

Cardoso

Campos

Pais³

et al. 2010

In Vitro Cell.Dev.Biol.-Plant

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Development and Applications of Somatic Embryogenesis in Grapevine (Vitis spp.)

Carra,

Wijerathna-Yapa,

Pathirana

et al. 2024

Plants

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Somatic embryogenesis (SE) provides alternative methodologies for the propagation of grapevine (Vitis spp.) cultivars, conservation of their germplasm resources, and crop improvement. In this review, the current state of knowledge regarding grapevine SE as applied to these technologies is presented, with a focus on the benefits, challenges, and limitations of this method. The paper provides a comprehensive overview of the different steps involved in the grapevine SE process, including callus induction, maintenance of embryogenic cultures, and the production of plantlets. Additionally, the review explores the development of high-health plant material through SE; the molecular and biochemical mechanisms underlying SE, including the regulation of gene expression, hormone signaling pathways, and metabolic pathways; as well as its use in crop improvement programs. The review concludes by highlighting the future directions for grapevine SE research, including the development of new and improved protocols, the integration of SE with other plant tissue culture techniques, and the application of SE for the production of elite grapevine cultivars, for the conservation of endangered grapevine species as well as for cultivars with unique traits that are valuable for breeding programs.

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Genotype Influence on Somatic Embryogenesis in Grapevine Anther and Leaf Cultures

Cited by 3 publications

References 0 publications

Regeneration of a wide range of African cassava genotypes via shoot organogenesis from cotyledons of maturing somatic embryos and conformity of the field-established regenerants

Regeneration of a wide range of African cassava genotypes via shoot organogenesis from cotyledons of maturing somatic embryos and conformity of the field-established regenerants

Use of morphometric parameters for tracking ovule and microspore evolution in grapevine (Vitis vinifera L., cv. “Aragonez”) and evaluation of their potential to improve in vitro somatic embryogenesis efficiency from gametophyte tissues

Development and Applications of Somatic Embryogenesis in Grapevine (Vitis spp.)

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