GenoType MTBDR
            <i>plus</i>
            Assay for Molecular Detection of Rifampin and Isoniazid Resistance in
            <i>Mycobacterium tuberculosis</i>
            Strains and Clinical Samples

Lacoma, Alícia; García-Sierra, Nerea; Prat, Cristina; Ruiz-Manzano, Juan; Haba, L.; Rosés, S; Maldonado, José; Domínguez, J.

doi:10.1128/jcm.00618-08

Cited by 112 publications

(88 citation statements)

References 26 publications

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“…In this study, sensitivity of the MTBDRplus assay for INH resistance detection was 95% (90-99%). In general, sensitivity of the MTBDRplus assay for INH resistance detection ranges from 73% to 92% [12][13][14]. Results of INH resistance detection were concor- [15,26].…”

Section: Discussionmentioning

confidence: 99%

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Assessment of the GenoType MTBDRplus assay for rifampin and isoniazid resistance detection on sputum samples in Cote d'Ivoire

N’guessan¹,

Assi²,

Ouassa³

et al. 2014

European Journal of Microbiology and Immunology

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We conducted an evaluation study on the GenoType MTBDRplus assay's ability to detect mutations conferring resistance to rifampin and isoniazid directly from sputum taken from 120 smear positive pulmonary patients from tuberculosis (TB) centers in Cote d'Ivoire.The sputum was decontaminated by N-acetyl-l-cysteine (NALC) and comparatively analyzed with the MTBDRplus assay version 2.0 and the mycobacterial growth indicator tube (MGIT) 960 automated drug susceptibility testing (MGIT-DST). The GeneXpert Mycobacterium tuberculosis (MTB)/rifampicin (RIF) assay was performed for 21 sputa with absence of hybridization for at least one rpoB wild-type probes. Four and seven, respectively, discordant and concordant results were also analyzed.The mutations in the rpoB gene were 21 (17.5%), 20 (16.7%), 7 (5.8%), and 10 (8.3%), respectively, for D516V, H526Y, H526D, and S531L. S315T mutation in katG gene associated or not with mutation in promoter of inhA was detected in 76 (63.3%) of the sputum. Compared to MGIT-DST, the sensitivity and specificity of the MTBDRplus for rifampin resistance detection were 100% (75-100%) and 73.2% (61.3-84%), respectively. For isoniazid resistance detection, the sensitivity and specificity were, respectively, 95% (90-99) and 95.1% (88.5-100%).Interpretation of 16 sputa without hybridization of rpoB wild-type probe 8 compared to those obtained with MGIT-DST and GeneXpert MTB/RIF was discordant and concordant, respectively, for 11 and 5.

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Section: Discussionmentioning

confidence: 99%

“…Similar studies conducted in Brazil [10] and Nigeria [11] showed that sensitivity of MTBDRplus for RMP resistance detection directly with sputum was of 82% and 83%, respectively. Sensitivity of MTBDRplus for RMP resistance detection ranges from 92% to 99% globally [12][13][14].…”

Section: Discussionmentioning

confidence: 99%

Assessment of the GenoType MTBDRplus assay for rifampin and isoniazid resistance detection on sputum samples in Cote d'Ivoire

N’guessan¹,

Assi²,

Ouassa³

et al. 2014

European Journal of Microbiology and Immunology

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“…Los resultados de sensibilidad y especificidad obtenidos en este estudio, tanto para RIf, INH y para ambas drogas simultáneamente (MDR,) fueron comparables con los resultados obtenidos por otras investigaciones (5,(9)(10)(11) demostrando su utilidad para la detección rápida de la resistencia a drogas antituberculosas. Los valores del índice de youden demuestran que la prueba molecular es muy eficiente para detectar la resistencia a las drogas antituberculosas, sobre todo cuando la muestra es esputo, siendo sus resultados muy confiables.…”

Section: Discussionunclassified

Prueba molecular genotype® MTBDRplus, una alternativa para la detección rápida de tuberculosis multidrogorresistente

Asencios

Galarza

Quispe

et al. 2014

Rev Peru Med Exp Salud Publica

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RESUMENLa prueba molecular Genotype®MTBDRplus, es un método que permite identificar las mutaciones más frecuentes asociadas con la resistencia a las drogas antituberculosas de primera línea: isoniacida (INH) y rifampicina (RIf). El objetivo de este estudio fue evaluar el desempeño de la prueba molecular con cultivos y muestras de esputo con baciloscopía positiva. Se evaluó 95 cultivos y 100 esputos con perfiles de resistencia previamente determinados por el método de referencia "proporciones agar en placa" (APP). La prueba molecular a partir de cultivos mostró una sensibilidad de 100%; 97,5% y 96,9% para RIf, INH y multidrogorresistente (MDR) respectivamente; mientras que para esputo la sensibilidad fue de 95,7%; 96,8% y 95,2% para RIf, INH y MDR respectivamente. Se concluye que Genotype®MTBDRplus es una herramienta muy útil para la detección rápida de la resistencia a INH y RIf simultáneamente (MDR) en un máximo de 72 h a partir de esputo o de cultivo. MOLECULAR TEST GENOTYPE® MTBDRPLUS, AN ALTERNATIVE TO RAPID DETECTION OF MULTIDRUG RESISTANCE TUBERCULOSIS ABSTRACTThe Genotype®MTBDRplus molecular test is a method that allows identification of the most frequent mutations associated with resistance to major first-line antituberculosis drugs, Isoniazid (INH) and Rifampicin (RfP). The aim of this study was to evaluate the performance of the molecular test with culture and smear-positive sputum samples. We evaluated 95 cultures and 100 sputum samples with resistance profiles previously determined by the reference method "Agar Plate Proportions" (APP). The molecular test from cultures showed a sensitivity of 100 %, 97,5 % and 96,97 % for RIf, INH and MDR respectively while from sputums the sensitivity was 95,65 %, 96,77 % and 95,24 % for RIf, INH and MDR respectively. We conclude that the molecular test Genotype®MTBDRplus is a very useful tool to detect resistance to isoniazid and rifampicin simultaneously (MDR-TB) in up to 72 hours from sputum samples or cultures.

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“…[32][33][34][35][36][37][38][39][40][41][42] Conceptually similar to the INNO-Lipa Rif.tb assay, the MTBDRplus assay has been reported to have a diagnostic sensitivity of approximately 94% for detecting M tuberculosis DNA in smear-positive respiratory specimens. 33 It is of interest that most clinical evaluations of this device have focused on its use for susceptibility testing, not detection of M tuberculosis, so the performance characteristics for the latter use are less well defined.…”

Section: Line Probe Assaysmentioning

confidence: 99%

Rapid Diagnosis of Mycobacterium tuberculosis Infection and Drug Susceptibility Testing

Wilson

2013

Archives of Pathology &Amp; Laboratory Medicine

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Context.—The global control of tuberculosis remains a challenge from the standpoint of diagnosis, detection of drug resistance, and treatment. This is an area of special concern to the health of women and children, particularly in regions of the world with high infant mortality rates and where women have limited access to health care. Objective.—Because treatment can only be initiated when infection is detected, and is guided by the results of antimicrobial susceptibility testing, there recently has been a marked increase in the development and testing of novel assays designed to detect Mycobacterium tuberculosis complex, with or without simultaneous detection of resistance to isoniazid and/or rifampin. Both nonmolecular and molecular assays have been developed. This review will summarize the current knowledge about the use of rapid tests to detect M tuberculosis and drug resistance. Data Sources.—Review of the most recent World Health Organization Global Tuberculosis Report, as well as selected publications in the primary research literature, meta-analyses, and review articles. Conclusions.—To a large extent, nonmolecular methods are refinements or modifications of conventional methods, with the primary goal of providing more rapid test results. In contrast, molecular methods use novel technologies to detect the presence of M tuberculosis complex and genes conferring drug resistance. Evaluations of molecular assays have generally shown that these assays are of variable sensitivity for detecting the presence of M tuberculosis complex, and in particular are insensitive when used with smear-negative specimens. As a group, molecular assays have been shown to be of high sensitivity for detecting resistance to rifampin, but of variable sensitivity for detecting resistance to isoniazid.

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GenoType MTBDR plus Assay for Molecular Detection of Rifampin and Isoniazid Resistance in Mycobacterium tuberculosis Strains and Clinical Samples

Cited by 112 publications

References 26 publications

Assessment of the GenoType MTBDRplus assay for rifampin and isoniazid resistance detection on sputum samples in Cote d'Ivoire

Assessment of the GenoType MTBDRplus assay for rifampin and isoniazid resistance detection on sputum samples in Cote d'Ivoire

Prueba molecular genotype® MTBDRplus, una alternativa para la detección rápida de tuberculosis multidrogorresistente

Rapid Diagnosis of Mycobacterium tuberculosis Infection and Drug Susceptibility Testing

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