Genotype of Varicella-Zoster Virus Isolates in South Korea

Kim, Kye-Hyung; Choi, Young Ju; Song, Kyoung Ho; Park, Wan Beom; Jeon, Jae Hyun; Kim, Hong Bin; Kim, Nam Joong; Oh, Myoung Don

doi:10.1128/jcm.02356-10

Cited by 12 publications

(9 citation statements)

References 18 publications

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“…Our results showed that the VZV strains isolated from patients in suburban Shanghai were primarily genotype J/clade 2 strains, suggesting that the distribution of VZV in China has significant geographical features. The results of our study, combined with those from studies in Japan (Inoue et al, 2010), Korea (Kim et al, 2011) and Mongolia (Enskhsaȋkhan et al, 2010), indicate that the majority of WT VZV strains in north-east Asia are genotype J/clade 2 strains. However, a small fraction of type M2 isolates were identified according to the 447 bp method in our study.…”

Section: Discussionsupporting

confidence: 79%

Genotype analysis of varicella-zoster virus isolates from suburban Shanghai Municipal Province, China

Sun

Guo

et al. 2016

Journal of Medical Microbiology

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We also observed significant inter-strain variations. Received 1 December 2014Accepted 8 December 2015 INTRODUCTIONThe genome of the varicella-zoster virus (VZV), a member of the family Herpesviridae and subfamily Alphaherpesvirinae, has been fully sequenced and found to consist of 125 kb of linear, dsDNA coding for 71 ORFs. VZV is the causative agent of varicella and herpes zoster (HZ) infections in humans. As a primary infection, varicella usually occurs in childhood, although it can be reactivated and manifest as HZ infection, or 'shingles', which typically occurs after the age of 50 years. Abbreviations: HZ, herpes zoster; SNP, single-nucleotide polymorphism; VZV, varicella-zoster virus.Two supplementary tables are available with the online Supplementary Material.

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Section: Discussionsupporting

confidence: 79%

Genotype analysis of varicella-zoster virus isolates from suburban Shanghai Municipal Province, China

Sun

Guo

et al. 2016

Journal of Medical Microbiology

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“…However, available data based on partial nucleotide sequences or restriction fragment length polymorphism suggest that all Korean isolates and Chinese isolates form a clade with Japanese isolates [16,17]. Thus, it is possible that the clade 2 could be extended to include China, which is geographically close to Japan and Korea.…”

Section: Discussionmentioning

confidence: 99%

Sequencing and characterization of Varicella-Zoster virus vaccine strain SuduVax

Kim

Jung

Kim

et al. 2011

Virol J

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BackgroundVaricella-zoster virus (VZV) causes chickenpox in children and shingles in older people. Currently, live attenuated vaccines based on the Oka strain are available worldwide. In Korea, an attenuated VZV vaccine has been developed from a Korean isolate and has been commercially available since 1994. Despite this long history of use, the mechanism for the attenuation of the vaccine strain is still elusive. We attempted to understand the molecular basis of attenuation mechanism by full genome sequencing and comparative genomic analyses of the Korean vaccine strain SuduVax.ResultsSuduVax was found to contain a genome that was 124,759 bp and possessed 74 open reading frames (ORFs). SuduVax was genetically most close to Oka strains and these Korean-Japanese strains formed a strong clade in phylogenetic trees. SuduVax, similar to the Oka vaccine strains, underwent T- > C substitution at the stop codon of ORF0, resulting in a read-through mutation to code for an extended form of ORF0 protein. SuduVax also shared certain deletion and insertion mutations in ORFs 17, 29, 56 and 60 with Oka vaccine strains and some clinical strains.ConclusionsThe Korean VZV vaccine strain SuduVax is genetically similar to the Oka vaccine strains. Further comparative genomic and bioinformatics analyses will help to elucidate the molecular basis of the attenuation of the VZV vaccine strains.

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“…Genotype M (including M1, M2, M3 and M4) is mainly detected in tropical regions in Africa and Central America [27,28]. Genotype J is the most prevalent VZV in the east Asia [29]. In the present study, one genotype J VZV strain was isolated from 26 samples with anti-VZV IgM positive.…”

Section: Discussionmentioning

confidence: 53%

Seroprevalence and molecular characteristics of varicella-zoster virus infection in Chinese children

Luan

Shen

Qiu

et al. 2019

Preprint

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Background Varicella-zoster virus (VZV) infection in children is an important public health problem in China. We performed the current study to explore the seroprevalence of VZV infection in Chinese children in order to provide more information for improvement of varicella vaccination in China. Methods 3014 serum samples were collected from Chinese kindergarten students aged from four to six years. Anti-VZV IgG and IgM were assayed using enzyme-linked immunosorbent assay. Both ORF22 and ORF62 of VZV were amplified, sequenced, and analyzed by nested PCR. Results Totally, 43.9% of boys and 46.3% of girls were vaccinated with varicella vaccine, respectively. The seroprevalence of anti-VZV IgG was 54.4% in the children with varicella vaccination, which was significantly higher than those in unvaccinated children (49.2%) (χ2=8.206, P=0.004). Among of the vaccinated children, the detection rates of VZV IgG antibody increased with age, with 49.4%, 50.9% and 58.9% in 4, 5 and 6-year groups, respectively (Trend χ2=17.202, P=0.002). However, there was no difference in anti-VZV IgG detection rates among those unvaccinated children in different age groups (Trend χ2=8.681, P=0.070). In addition, thirteen boys and 13 girls were positive for anti-VZV IgM, respectively. Among of them, eight children (0.6%) have received varicella vaccination, which was similar to those in unvaccinated children (1.1%). However, only one ORF22 sequence was isolated from an unvaccinated 5-year boy. Compared to the reference VZV sequences, the nucleotide homology was estimated to be 99.7% with genotype J. Conclusions Our study indicated that about half of Chinese children aged four to six years have a high risk of VZV infection. It should be helpful for the evaluation on the necessity of varicella immunization in China.

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Genotype of Varicella-Zoster Virus Isolates in South Korea

Cited by 12 publications

References 18 publications

Genotype analysis of varicella-zoster virus isolates from suburban Shanghai Municipal Province, China

Genotype analysis of varicella-zoster virus isolates from suburban Shanghai Municipal Province, China

Sequencing and characterization of Varicella-Zoster virus vaccine strain SuduVax

Seroprevalence and molecular characteristics of varicella-zoster virus infection in Chinese children

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