Genotype profiles of Mycobacterium avium subspecies paratuberculosis isolates recovered from animals, commercial milk, and human beings in North India

Abstract

This study is the first from India to report the presence of two kinds of MAP genotypes (cattle-type and bison-type). However, non-reporting of the sheep-type genotype may not mean that it is absent in North India; the use of multiple culture media to recover MAP from clinical samples for future investigations is advised.

Diagnostic Potential of Three Antigens from Geographically Different Regions of the World for the Diagnosis of Ovine Johne’s Disease in India

“…Like our study in sheep, JTC-ELISA developed by Shin et al (2008) was particularly sensitive at detecting low level fecal shedders of MAP (sub-clinical infection) and works effectively both on serum and milk samples for the detection of cattle infected with sub-clinical MAP infections, providing a cost-effective diagnostic tool to support JD control programs in cattle herds. Unlike Cho and Collins (2006) reporting that protein antigens of sero-diagnostic potential were more abundant in culture filtrates than cellular extracts from liquid culture of MAP, they used protein based species specific semi-purified whole cell PPA from cellular part of novel native MAP biotype (Indian Bison Type) cultured on solid HEY medium and was highly sensitive and specific (Singh et al, 2007b(Singh et al, , 2009a. Kumar et al (2006) reported that use of species specific antigens from 'Indian Bison Type' MAP showed better cross reactivity i.e., using PPA from sheep isolate in sheep rather than goat origin PPA in sheep.…”

Genetic Structure of Mycobacterium avium subsp. paratuberculosis Population in Cattle Herds in Quebec as Revealed by Using a Combination of Multilocus Genomic Analyses

“…The amplicon size was 608 bp. REA reactions were performed as described previously (18,29). Briefly, restriction reactions were carried out in a volume of 30 l, containing 20 l of positive IS1311 PCR, 3 l of reaction 10ϫ buffer, and 2 U of endonucleases HinfI and MseI.…”

Mycobacterium avium ssp . paratuberculosis detection in animals, food, water and other sources or vehicles of human exposure: A scoping review of the existing evidence

“…Most of these studies were based on existing isolate collections, and showed a fair amount of homogeneity within M. paratuberculosis strains and revealed that M. paratuberculosis strains from human infections were less diverse compared to cattle (Wynne et al, 2011). Results were derived from several methods making it difficult to make direct comparisons, including IS1311 restriction fragment length polymorphism (RFLP) analyses (Whittington et al, 2000;Motiwala et al, 2003;Singh et al, 2010b;Okuni et al, 2012;Liapi et al, 2015), (Stevenson et al, 2009;Fernandez-Silva et al, 2012) randomly amplified polymorphic DNA (RAPD) (Pillai et al, 2001), multi-locus short sequence repeat sequencing (MLSSR) (Fernandez-Silva et al, 2012), Multiplex PCR of IS900 integration loci (MPIL) (Motiwala et al, 2004), short sequence repeats (SSR) (Ghadiali et al, 2004), and single nucleotide polymorphisms (SNPs) (Wynne et al, 2011). A tally of the type of M. paratuberculosis (e.g.…”

“…cattle, sheep, bison) found by species category is shown in Figure 4. Multiple fingerprinting techniques were used to show the diversity of M. paratuberculosis within populations, and it was argued that it is possible to demonstrate strain sharing within and across species by the degree of homogeneity between isolates (Stevenson et al, 2009 (Singh et al, 2010b;Sohal et al, 2014;Ahlstrom et al, 2015;Podder et al, 2015).…”