Genotype specificity of the somatic embryogenesis response in cotton

Trolinder, Norma L.; Xhixian, Chen

doi:10.1007/bf00716824

Cited by 124 publications

(77 citation statements)

References 10 publications

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“…These results are similar to those in (Zhang et al, 2001;Trolinder and Xhixian, 1989) who classified cotton varieties on the basis of somatic embryogenesis and plant regeneration into four categories. First were the varieties with high ability for somatic embryogenesis and plant regeneration, such as Coker 201, Coker 312 which have become the model varieties in cotton tissue culture and genetic transformation.…”

Section: Fig4 Compact Callus Of G Anomalum On Cim Mediumsupporting

confidence: 91%

“…Shoemaker et al(1986) described somatic embryogenesis and plant regeneration from G. hirsutum var Coker 315 and 201. Trolinder and Xhixian (1989) obtained regenerated plants from cell suspensions of 38 genotypes and classified them into four classes as high, moderate, low and non embryogenic. Zhang et al(1996) observed somatic embryogenesis and regenerated plants from Coker 201.…”

Section: Introductionmentioning

confidence: 99%

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Somatic embryogenesis in wild relatives of cotton (Gossypium Spp.)

Rao

Hussain

Shahzad

et al. 2006

J. Zhejiang Univ. - Sci. B

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Abstract:Wild cotton species can contribute a valuable gene pool for agronomically desirable cultivated tetraploid cultivars. In order to exploit diploid cotton a regeneration system is required to achieve transformation based goals. The present studies aimed at optimizing the conditions for regeneration of local varieties as well as wild species of cotton. Different callus induction media were tested with varying concentrations of hormones in which sucrose was used as nutritional source. Different explants (hypocotyls, cotyledon, root) were used to check the regeneration of both local cotton plants and wild relatives using T & G medium, BAP medium, CIM medium, EMMS medium, and cell suspension medium. Different stages of embryogenicity such as early torpedo stage, late torpedo stage, heart stage, globular stage and cotyledonary stage were observed in wild relatives of cotton. The results of this study pave the way for establishing future transformation methods.

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Section: Fig4 Compact Callus Of G Anomalum On Cim Mediumsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis in wild relatives of cotton (Gossypium Spp.)

Rao

Hussain

Shahzad

et al. 2006

J. Zhejiang Univ. - Sci. B

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“…Regeneration response in cotton is highly genotype specific (Trolinder and Xhixian, 1989). Most first-and second-generation GM cottons, including Bollgard ® , were developed through the incorporation of transgenes in regeneration-responsive genotypes and then transferred to the desirable genetic background through conventional means.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity analysis of Bt cotton genotypes in Pakistan using simple sequence repeat markers

Ullah¹,

Iram²,

Iqbal³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. The popularity of genetically modified insect resistant (Bt) cotton has promoted large scale monocultures, which is thought to worsen the problem of crop genetic homogeneity. Information on genetic diversity among Bt cotton varieties is lacking. We evaluated genetic divergence among 19 Bt cotton genotypes using simple sequence repeat (SSR) markers. Thirty-seven of 104 surveyed primers were found informative. Fifty-two primers selected on the basis of reported intra-hirsutum polymorphism in a cotton marker database showed a high degree of polymorphism, 56% compared to 13% for randomly selected primers. A total of 177 loci were amplified, with an average of 1.57 loci per primer, generating 38 markers. The amplicons ranged in size from 98 to 256 bp. The genetic similarities among the 19 genotypes ranged from 0.902 to 0.982, with an average of 0.947, revealing a lack of diversity. Similarities among genotypes from public sector organizations were higher than genotypes developed by private companies. Hybrids were found to be more distant compared to commercial cultivars and advanced breeding lines. Cluster analysis grouped the 19 Bt cotton genotypes into three major clusters and two independent entries. Cultivars IR-3701, Ali Akbar-802 and advanced breeding line VH-259 grouped in subcluster B2, with very narrow genetic distances despite dissimilar parentage. We found a very high level of similarity among Pakistani-bred Bt cotton varieties, which means that genetically diverse recurrent parents should be included to enhance genetic diversity. The intra-hirsutum polymorphic SSRs were found to be highly informative for molecular genetic diversity studies in these cotton varieties.

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“…Since all the cotton genotypes are not amenable through tissue culture method therefore all the transformed cells cannot be cultured to develop plants. It is because of genotype-specificity of cotton towards callus culture (Trolinder and Xhixian, 1989). Genotype-specificity is a genetic trait and is controlled by quantitative genes (Gawel and Robacker, 1990).…”

Section: Discussionmentioning

confidence: 99%

Introgression of G. barbadense genes into G. hirsutum through DNA-mediated Embryo Transformation approach

Aslam¹,

.²,

Iqbal³

1997

Pakistan J. of Biological Sciences

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Attempts were made to incorporate intraploid interpsecific gene(s) from Gossyium barbadense into G. hirsutum through DNA-mediated embryo transformation approach. G. barbadense DNA was injected into the styles/ovaries of G. hirsutum, 24 hours after self pollination. Two phenotypic types of plants i.e. transformed and G. hirsutum like were observed in D population. The transformed plants had clearcut variations in boll weight, yield and other quality traits confirmed to recipient parent. D transformations were also heritable and were transmitted to D generation. The transformed plants had better boll weight (4.9 gm), better fibre fineness (3.9 µg/in) and better fibre strength (101.0 TPPSI) compared to the boll weight (3.0 gms), fibre fineness (4.5 µg/in) and fibre strength (91.0 TPPSI) of G. hirsutum. The transformation efficiency for quantitative traits was about 20 % where as low for qualitative traits like flower petals with red spot (0.08 %). In the present studies transformed genotypes illustrated higher yield potential and better fibre quality than the recipient (G. hirsutum) parent.

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Genotype specificity of the somatic embryogenesis response in cotton

Cited by 124 publications

References 10 publications

Somatic embryogenesis in wild relatives of cotton (Gossypium Spp.)

Somatic embryogenesis in wild relatives of cotton (Gossypium Spp.)

Genetic diversity analysis of Bt cotton genotypes in Pakistan using simple sequence repeat markers

Introgression of G. barbadense genes into G. hirsutum through DNA-mediated Embryo Transformation approach

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