Genotypic Analysis of Cutaneous T-Cell Lymphomas

Ralfkiær, Elisabeth; O’Connor, Nathan; Crick, J.; Wantzin, Gunhild Lange; Mason, David Y.

doi:10.1111/1523-1747.ep12470458

Cited by 77 publications

(16 citation statements)

References 29 publications

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“…In the past, Southern blot techniques, [8][9][10][11][12][13][14][15][16][17][18][19] flow cytometry, 20 reverse transcription PCR, 21,22 and PCRdenaturing gradient gel electrophoresis 23 have been used, with varying success, to detect T-cell receptor gene rearrangements in frozen or fresh tissue or peripheral blood from patients with cutaneous T-cell lymphoma. In general, these studies have analysed TCR-, TCR-, and TCR-, and have shown gene rearrangements in plaque or tumour stage mycosis fungoides.…”

Section: Discussionmentioning

confidence: 99%

The polymerase chain reaction in the diagnosis of early mycosis fungoides

et al. 1997

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The earliest or patch stage of mycosis fungoides may present diagnostic difficulty both clinically and pathologically. The present study of the polymerase chain reaction (PCR) as a diagnostic tool in early mycosis fungoides was therefore undertaken, using a rapid PCR method for the detection of γ‐ and β‐chain T‐cell receptor (TCR) gene rearrangements in routine formalin‐fixed, paraffin‐embedded histological sections. Forty‐two biopsies were studied from 26 patients with mycosis fungoides. Twenty‐three skin biopsies with a clinicopathological diagnosis of early, or patch stage, mycosis fungoides were investigated. Of these, 18 (78 per cent) showed TCR‐γ or both β‐ and γ‐chain TCR gene rearrangements. TCR gene rearrangements were shown in seven of the 14 plaque stage lesions (50 per cent) and also in the single case of tumour stage disease. Where gene rearrangements were identified, these were identical in all biopsies from an individual patient, irrespective of the site of the lesion, the disease stage, or the time lapse between the biopsies. The PCR is therefore a highly sensitive technique, which can be performed on routine pathological material, in cases where the diagnosis of early mycosis fungoides cannot be made with certainty on conventional histopathological and immunohistochemical grounds. © 1997 John Wiley & Sons, Ltd.

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Section: Discussionmentioning

confidence: 99%

The polymerase chain reaction in the diagnosis of early mycosis fungoides

et al. 1997

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“…These limitations have been particularly problematic in the genotypic analysis of minimally infiltrated tissues, such as early mycosis fungoides (MF) skin lesions, that contain relatively few T cells. The inability to detect clonal rearrangements by Southern blot analysis has been reported for many such early MF lesions [8,18,30]. Initially, it was suggested that these lesions might be truly polyclonal; however, as detailed below, recent evidence suggests that early MF lesions do, in fact, contain dominant T cell tumor clones but at a level low enough to frequently escape detection by Southern blot analysis.…”

Section: Limitations Of Southern Blot Analysismentioning

confidence: 99%

“…Southern blot analysis of TCR gene rearrangements has reliably demonstrated the monoclonality of well-developed lymphoma lesions such as thick plaques and tumor nodules of MF or tumor nodules of other cutaneous T cell lymphomas [25][26][27]. Nevertheless, Southern blot analysis has been unable to consistently demonstrate the clonality of early skin lymphoma lesions such as the patch phase of MF [8,18,30]. Rather than indicating that such lesions are truly polyclonal, it now appears that such patches are in fact monoclonal lesions that fall within region A or B in Fig.…”

Section: Early Diagnosis and Staging Of Cutaneous Lymphoproliferativementioning

confidence: 99%

Cutaneous lymphoproliferative disorders: strategies for molecular biological analysis and their major findings

Wood

1992

Springer Semin Immunopathol

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This article focuses on the principal molecular biological methods that have been devised to study cutaneous lymphoid infiltrates. These methods involve analysis of antigen receptor gene rearrangements, chromosomal translocations and proviral integration using Southern blot analysis and gene amplification techniques. The major findings are discussed that pertain to early diagnosis, staging, disease monitoring, the development of new immunopathological assays and the detection of tumor-associated retroviruses.

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“…Struc tural alterations of the c-myc gene, which encodes a pro tein that is functionally involved in DNA synthesis [6], have been detected in lymphoblastic leukemias [7] and large-cell lymphomas (8] but not in primary cutaneous follicular B cell lymphomas [9]. Furthermore, several T cell neoplasms including mycosis fungoides, Sezary syn drome, T cell lymphocytic leukemia and nodal T cell lym phomas have now been studied with immunogenotyping techniques and showed T cell receptor gene rearrange ment in a clonal fashion [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Expression of c-myc in Cutaneous Lymphomas and Pseudolymphomas

et al. 1991

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The expression of the proto-oncogene c-myc was studied in tumorous skin lesions of (cutaneous) lymphoproliferative diseases (3 cases of pseudolymphoma, 8 cases of non-Hodgkin lymphoma and 1 case of lymph node involvement in mycosis fungoides) in a total of 12 patients, c-myc mRNA levels were quantified by Northern blot analysis followed by densitometric evaluation of the specific bands. Higher levels of c-myc mRNA expression were observed in lymphomas as compared with pseudolymphomas (p > 0.05). No significant differences in c-myc mRNA values were detected between B and T cell pseudolymphomas. Our results suggest that c-myc mRNA overexpression is associated with malignant lymphomas of the skin. We conclude that the measurement of c-myc mRNA levels may contribute to further characterize cutaneous lymphoproliferative diseases.

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Genotypic Analysis of Cutaneous T-Cell Lymphomas

Cited by 77 publications

References 29 publications

The polymerase chain reaction in the diagnosis of early mycosis fungoides

The polymerase chain reaction in the diagnosis of early mycosis fungoides

Cutaneous lymphoproliferative disorders: strategies for molecular biological analysis and their major findings

Expression of c-myc in Cutaneous Lymphomas and Pseudolymphomas

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