Genotypic analysis of Giardia duodenalis in children at Egypt

Ghieth, Marwa A.; El-Badry, Ayman A.; Abu-Sarea, Enas Y.; Gawad, Samah S. Abdel; Elsharkawy, Marwa

doi:10.1007/s00580-016-2337-7

Cited by 9 publications

(3 citation statements)

References 40 publications

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“…In the present study, stool samples were collected from 165 children. Microscopy-based prevalence of giardiasis was 24.2%, which is consistent with previous estimates in Egypt that range from 24.7% to 27.9% [ 34 , 35 ]. Other studies report lower and higher prevalence rates [ 36 , 37 ].…”

Section: Discussionsupporting

confidence: 90%

Genotyping of Giardia duodenalis in children in upper Egypt using assemblage- specific PCR technique

Ahmad¹,

El-Kady²,

Hassan³

2020

PLoS ONE

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Giardia duodenalis is a common gastrointestinal protozoan parasite, causing diarrheal illness in humans worldwide. Yet, the distribution of G. duodenalis genotypes among human patients and their clinical relevance remains controversial. This study aimed to detect G. duodenalis in children in Upper Egypt and identify causative genotypes and elucidate a possible correlation between genotype and clinical presentation. One hundred sixty-five children, regardless of symptoms, were tested for giardiasis. Giardia positive stool samples (40/165) were subjected to PCR amplification targeting the tpi gene with positive PCR results in only 35 cases (87.5%). Assemblage-specific amplification of genotypes (A, B, and the zoonotic E strains) revealed predominantly G. duodenalis Assemblage A (45.7%). Assemblage B and mixed A and B infections were detected in 31.4% and 22.8% of children, respectively. Assemblage E was not detected. G. duodenalis assemblage A was dominant in children who complained of diarrhea and abdominal cramps. In contrast, asymptomatic children with positive stool samples display a higher frequency of assemblage B and mixed infections. The study highlights the predominance of Giardia Assemblage A in our study locality. This study is the first for this endemic area to use the copro-PCR technique for diagnosis and genotyping of giardiasis. Study results show the value of simple species-specific primers for genotyping in communities with little access to laboratory resources. Further genetic studies are needed to clarify the association between parasite genetic diversity and patient symptomatology.

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Section: Discussionsupporting

confidence: 90%

Genotyping of Giardia duodenalis in children in upper Egypt using assemblage- specific PCR technique

Ahmad¹,

El-Kady²,

Hassan³

2020

PLoS ONE

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“…Also from Egypt, El Tantawy and Taman [15] , targeting the tpi gene, reported 62.14% as assemblage B, 31.07% as A, and mixed A and B in 2.91% with 3.88% of undetermined species. Hence with variable reports on prevalence, assemblage B seems to be the most prevailing assemblage for Giardia infections in Egypt as documented in many Egyptian studies in Cairo [2,28] , Kafr Elshiekh [29] , and Ismailia [13,30] .…”

Section: Discussionmentioning

confidence: 99%

Predominance of Giardia intestinalis assemblage B in diarrhoeic children in Sharkia, Egypt

El-Badry

Mohammed

Gawad

2017

Parasitologists United Journal

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Background: Giardia intestinalis (G. intestinalis) is one of the most common human enteric protozoa that infect Egyptian children, causing most of the diarrhoeic outbreaks worldwide. Identification of G. intestinalis assemblage is important because of its role in determining sources of infection, in understanding the ecological and clinical impact of giardiasis, and hence its management and control. Objective: Study of this cross-sectional sample aimed to determine the genotypes of G. intestinalis isolated from the feces of diarrhoeic children attending outpatient clinic of Zagazig University hospital. Subjects and Methods: A single fecal sample was collected from each child (n=126). All samples were subjected to microscopic examination by direct wet mount before and after formal-ethyl acetate concentration. Positive samples were amplified by nested PCR (nPCR) and sequenced for intra and inter assemblage identification targeting tpi gene. Results: Microscopic examination revealed detection of G. intestinalis in 38 samples (30.2%). In 36 (28.6%) samples there was assemblage B predominance in 34 (94.4%) isolates, among which 24 (70.6%) were subgenotype BIV and 10 (29.4%) were sub-genotype BIII. Only 2 (5.6%) samples had assemblage A; all of them were AII subgenotype. All studied patient`s demographic and clinical data showed no significant association with Giardia infection or prevailing Giardia assemblages. Conclusion: G. intestinalis is the prevailing intestinal pathogen in diarrhoeic Egyptian children, with Giardia assemblage B predominance. These findings necessitate physicians' attention and further genetic studies in Egypt and other endemic areas targeting different genetic loci, with the inclusion of larger population samples. This will lead to a better understanding of the ecological and clinical impact of giardiasis, its management and control. MATERIALS AND METHODS Study type and populations: This cross-sectional study sample included 126 children (5 to 12 years old),

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“…As to giardiasis in renal-free healthy individuals, Ghieth et al (2016) in Beni-Suef Governorate, Sadek et al (2013), Fahmy et al (2015) in Menoufiya Governorate and El-Tantawy and Taman (2014) in Dakahlia Governorate reported a higher prevalence (27.9%, 35%, 33.1%, & 30%, respectively). But, El Beshbishi et al (2005) and El- Naggar et al (2006) in Dakahlia Governorate reported lower frequency (2.3%, & 7.9%).…”

Section: Resultsmentioning

confidence: 99%

Co-Infection of Giardia Lamblia and Helicobacter Pylori Infection Among Chronic Kidney Diseased Patients Undergoing Hemodialysis in Beni-Suef University Hospitals

El-Askary¹,

Ismail²,

El-Ghareeb³

et al. 2021

Journal of the Egyptian Society of Parasitology

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Giardia lamblia and Helicobacter pylori are two microorganisms that grow in duodenum and stomach; and sharing the same mode of infection. Chronic kidney disease (CKD) is an end stage disease causing uremia that requires hemodialysis (HD). The association of Giardia lamblia and H. pylori infection has been known to be common and hemodialysis may play an important role on this co-infection. This study evaluated the interrelation of Giardia lamblia and H. pylori in patients of CKD treated with hemodialysis.A case-control study performed on two hundred stool samples collected from patients attending Beni-Suef University Hospital suffering from diarrhea and other GIT symptoms. One hundred patients suffering from CKD and treated with hemodialysis and a hundred control group with normal kidney functions of both genders. Both groups were subjected to coproparasitological examination and fecal immuno-assays.The results showed that Giardia in 13 CKD patients with a mean age of 45.24±14.52 and in 22 cross-matched control patients. Males showed prevalent of (66%), who were from rural areas (66.5%) and using tap water (83.5%). H. pylori infection was in 22 patients CKD and in 27 control patients. Co-infection was found in 10 CKD patients and 19 of control.

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Genotypic analysis of Giardia duodenalis in children at Egypt

Cited by 9 publications

References 40 publications

Genotyping of Giardia duodenalis in children in upper Egypt using assemblage- specific PCR technique

Genotyping of Giardia duodenalis in children in upper Egypt using assemblage- specific PCR technique

Predominance of Giardia intestinalis assemblage B in diarrhoeic children in Sharkia, Egypt

Co-Infection of Giardia Lamblia and Helicobacter Pylori Infection Among Chronic Kidney Diseased Patients Undergoing Hemodialysis in Beni-Suef University Hospitals

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