Genotypic and allelic distribution of IFN‐γ +874T/A and TGF‐β1 −509C/T single‐nucleotide polymorphisms in human  immunodeficiency virus‐infected Thais

Abstract

Advances in antiretroviral therapy (ART) have led to a decrease of acquired immunodeficiency syndrome (AIDS)-related mortality, and an increase of non-AIDS illnesses in people living with HIV (PLWH). Risks for HIV-related chronic inflammation leading to non-AIDS illnesses in PLWH have been increasingly clar-

Lack of Association between IFN-γ, CXCL10 and TGF-β1 Gene Polymorphisms and Liver Complication in HIV-infected Thais

“…Genotypic and allelic frequencies of IFN-γ +874 T/A, CXCL10 G-201A/C-1596T and TGF-β1 -509C/T SNPs were previously reported (Table 3) (Akekawatchai et al, 2022;Tunkor et al, 2018). The distribution of all SNP alleles and genotypes were consistent with the Hardy-Weinberg equilibrium (p ˃ 0.05).…”

“…DNA samples were genotyped for IFN-γ +874 T/A, CXCL10 G-201A/C-1596T and TGF-β1 -509C/T SNPs using different PCR-based assays. The genotyping of +874 T/A SNP was performed by a specific sequence primer polymerase chain reaction (SSP-PCR) (Ghasemian and Shahbazi, 2016;Akekawatchai et al, 2022), while that of G-201A and C-1596/T SNPs were analyzed by PCR-restriction fragment length polymorphism (PCR-RFLP) as described (Limothai et al, 2016;Yang et al, 2013;Tunkor et al, 2018). The TGF-β1 -509C/T SNP was genotyped using amplification refractory mutation system-PCR (ARMS-PCR) assay (Heidari et al, 2013;Akekawatchai et al, 2022).…”

Recent Progress in Single-Nucleotide Polymorphism Biosensors

“…Single-nucleotide polymorphisms (SNPs) are the most common form of genetic variations in human genomes [ 1 , 2 , 3 , 4 ]. SNPs were first discovered in 1980; restriction endonuclease assays were used to determine the presence or absence of DNA cleavage sites in the past [ 5 ].…”