Genotypic and Phenotypic Characterization of IncX3 Plasmid Carrying blaNDM-7 in Escherichia coli Sequence Type 167 Isolated From a Patient With Urinary Tract Infection

Abstract

Infections due to New Delhi metallo-beta lactamase (NDM)-7-producing Escherichia coli are infrequent and sporadic. In this study, we report one case of recurrent urinary tract infection caused by blaNDM-7-producing E. coli belonging to phylogenetic group A, sequence type (ST) 167. In this study, we aimed to describe the genotype and phenotype of blaNDM-7-producing E. coli in China. The isolate exhibited resistance to β-lactam antimicrobials, trimethoprim-sulfamethoxazole, quinolones, and aminoglycosides. blaNDM-7 is located on a conjugative plasmid designated pJN05NDM-7 belonging to type IncX3. pJN05NDM-7 was fully sequenced and compared with all publicly available blaNDM-7-harboring plasmids. pJN05NDM-7 is almost identical to pKpN01-NDM7 and pKW53T, although the plasmids are geographically unrelated. The comparison of IncX3 plasmids harboring blaNDM in China showed high similarity, with genetic differences within insertion fragments. Notably, the differences in plasmids of animal and human origin were insignificant, because only one plasmid showed deletion inside the ISAba125 region compared with pJN05NDM7. Our study demonstrates that E. coli carrying IncX3 plasmids play an important role as a reservoir and in the spread of blaNDM. Further studies should be performed to control the dissemination of blaNDM among food animals.

Genotypic and Phenotypic Characterization of blaNDM–7-Harboring IncX3 Plasmid in a ST11 Klebsiella pneumoniae Isolated From a Pediatric Patient in China

“…Conjugation test revealed that only bla NDM– 7 could be horizontally transferred, and the sequencing results showed that plasmid IncX3 harbored by B14 does not carry antimicrobial resistance genes other than bla NDM– 7 . This finding corroborates the results of Hao et al (2018) .…”

“…The size and amount of plasmids carried by the clinical isolate and transconjugant were evaluated using S1-pulsed-field gel electrophoresis (S1-PFGE) as previously described ( Hao et al, 2018 ). The genome of Salmonella H9812 digested with Xba I was used as the marker.…”

“…To date, NDM-7-producing enterobacteria have been reported in more than 10 countries ( Solgi et al, 2017 ; Ahmad et al, 2018 ; Yoon et al, 2018 ; Mouftah et al, 2019 ). In China, NDM-7 has been detected and described in E. coli but not in K. pneumoniae ( Wang et al, 2016 ; Bi et al, 2018 ; Hao et al, 2018 ; Xu and He, 2019 ). In this study, we isolated a ST11 K. pneumoniae harboring bla NDM– 7 from a boy hospitalized in the pediatric intensive care unit of a Chinese Hospital.…”

“…Genotypic and phenotypic characterization of the IncX3 plasmid carrying bla NDM– 7 gene was performed. In 2015, an IncX3 plasmid carrying bla NDM– 7 was isolated from E. coli in the same hospital ( Hao et al, 2018 ). To analyze the evolution of IncX3 plasmid carrying bla NDM– 7 , we constructed a phylogenetic tree from 16 plasmids based on homologous proteins.…”

Characterization of the IncX3 Plasmid Producing blaNDM–7 From Klebsiella pneumoniae ST34

“…In China, CRE strains are mainly composed of KPC-producing K. pneumoniae and NDM-producing E. coli ( Zhang et al, 2017 , 2018 ). NDM-7 has been reported in E. coli strains ( Hao et al, 2018 ). Recently, NDM-7 carrying the IncA/C2 type plasmid harbored by K. pneumoniae strain ST147 was reported ( Shankar et al, 2019 ).…”