Genotypic and phenotypic characterization of Pseudomonas aeruginosa isolates from urinary tract infections

Tielen, Petra; Narten, Maike; Rosin, Nathalie; Biegler, Ilona; Haddad, Isam; Hogardt, Michael; Neubauer, Rüdiger; Schobert, Max; Wiehlmann, Lutz; Jahn, Dieter

doi:10.1016/j.ijmm.2010.10.005

Cited by 48 publications

(44 citation statements)

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“…Urinary tract infections (UTIs) are one of the most common types of both community- and hospital-acquired infections, and have a serious impact on morbidity and mortality, with a significant financial burden in terms of treatment costs [1,2,3]. Pseudomonas aeruginosa plays a significant role in complicated and catheter-associated UTIs, as it is the third most common pathogen associated with UTIs [4,5,6].…”

Section: Introductionmentioning

confidence: 99%

“…Pseudomonas aeruginosa plays a significant role in complicated and catheter-associated UTIs, as it is the third most common pathogen associated with UTIs [4,5,6]. These infections are difficult to treat because of the high level of antibiotic resistance amongst P. aeruginosa strains; they may become persistent or chronic, which can lead to potentially fatal sepsis [1,2]. A growing number of reports describe multidrug resistance (MDR) in P. aeruginosa , and strains producing metallo-β-lactamases (MBLs) are of particular concern [7].…”

Section: Introductionmentioning

confidence: 99%

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Virulence and Antibiotic Resistance of Pseudomonas aeruginosa Isolated from Patients with Urinary Tract Infections in Southern Poland

Pobiega

Wójkowska‐Mach²,

Maciąg³

et al. 2014

Chemotherapy

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Background: The aim of this study was to analyze the resistance and virulence of Pseudomonas aeruginosa strains causing urinary tract infections in in- and outpatients in Southern Poland. Methods: The study included 83 inpatients and 66 outpatients; 36.9% were female. Results: Monomicrobial infections accounted for 74.5%; polymicrobial infections occurred more frequently among inpatients (odds ratio, OR = 4.32, p = 0.0008). exoS and lasB were detected in 90 and 74% of isolates, respectively. aprA was present in 66%, pilB in 5% and pilA in 23% of isolates. Isolates from adults were more frequently resistant to fluoroquinolones (OR = 0.37, p = 0.029). Twenty-nine isolates were classified as multidrug resistant and 12 as extremely drug resistant, which occurred less frequently in patients <17 years (OR = 0.18, p = 0.024). Nine metallo-β-lactamase-positive isolates were identified. bla_SHV was present in 10, bla_TEM in 6, bla_OXA-10 in 3 and bla_VIM-2 in 3 isolates. Conclusion: Antibiotic selection should be based on the knowledge of local antimicrobial susceptibilities to maximize the benefit for patients and minimize the risk of drug resistance.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Virulence and Antibiotic Resistance of Pseudomonas aeruginosa Isolated from Patients with Urinary Tract Infections in Southern Poland

Pobiega

Wójkowska‐Mach²,

Maciąg³

et al. 2014

Chemotherapy

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“…This is consistent with the work of others (Pirnay et al 2005;Wiehlmann et al 2007;Tielen et al 2011;Kidd et al 2012;Workentine et al 2013). There is no dominant group or phenotype of P. aeruginosa that has been associated with any environment (Wiehlmann et al 2007).…”

Section: Environment Of Isolationsupporting

confidence: 93%

Metabolic profiles of household Pseudomonas species groups.

Onyiri

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_________________________________________Resources influence the distribution, behavior, growth and reproduction of bacterial species. Understanding how closely related species utilize resources will shed light on the ecological traits or principles that shape biogeographic patterns and promote coexistence of species. Twelve Pseudomonas strains isolated from a single human home and PA01, the standard laboratory strain were evaluated in Luria Bertani media to understand whether their growth traits are influenced by their species group and/or their environment of isolation. Total growth, growth rate, early cumulative area under the growth curve (CUC) over 48 hours and viability (viable bacteria count per ml) at 24 and 48 hours were evaluated for each strain. I found differences in growth strategies that may be driven by species group and/or environment of isolation. These results suggest local adaptation of Pseudomonas strains to human home environments.These strains were also evaluated for their metabolic and oxidative profiles in 31 resources as a way to understand if metabolic and/or oxidative profiles could be predicted by their species group and/or their environment of isolation. Metabolic vi profile for each strain was evaluated as the qualitative ability to use a resource coded as 0 or 1, while the oxidative profile in each resource is a quantitative measure that was determined by integrating the CUC for each resource after 48 hours of growth. I found some resources were utilized by all the strains, suggesting similar metabolic adaptations or common ancestry. I also found evidence for resource differentiation that was driven by species group, but very little evidence for environment of isolation or local adaptation.Thus, resource differentiation can reduce competition and promote coexistence of species.

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“…This adaptability is believed to give the variants a selective advantage under unfavorable conditions. Clinical isolates of Pseudomonas have been reported to display a high degree of phenotypic diversity (8)(9)(10), especially those isolated from antibiotic treated patients. As a result of its high adaptability (11) and intrinsic antibiotic resistance (12), infections caused by P. aeruginosa are, in many instances, difficult to eradicate and can become persistent or even chronic (10), earning this bacterium a reputation as a paradigm of bacterial resistance (13).…”

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confidence: 99%

Predation in Homogeneous and Heterogeneous Phage Environments Affects Virulence Determinants of Pseudomonas aeruginosa

Hosseinidoust

Tufenkji

Ven

2013

Appl Environ Microbiol

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bThe rise of bacterial variants in the presence of lytic phages has been one of the basic grounds for evolution studies. However, there are incongruent results among different studies investigating the effect of phage resistance acquisition on bacterial fitness and virulence. We used experimental evolution to generate three classes of Pseudomonas aeruginosa variants under selective pressure from two different homogeneous phage environments and one heterogeneous phage environment. The fitness and virulence determinants of the variants, such as growth, motility, biofilm formation, resistance to oxidative stress, and the production of siderophores and chromophores, changed significantly compared to the control. Variants with similar colony morphology that were developed through different phage treatments have different phenotypic traits. Also, mRNA transcription for genes associated with certain phenotypic traits changed significantly; however, sequencing did not reveal any point mutations in selected gene loci. Furthermore, the appearance of small colony variants and melanogenic variants and the increase in pyocyanin and pyoverdin production for some variants are believed to affect the virulence of the population. The knowledge gained from this study will fundamentally contribute to our understanding of the evolutionary dynamics of bacteria under phage selective pressure which is crucial to the efficient utilization of bacteriophages in medical contexts. Pseudomonas aeruginosa is a metabolically versatile gammaproteobacterium which inhabits terrestrial, aquatic, animal, human, and plant host-associated environments (1). This pathogen is endowed with a fairly large genome (2) containing genes for many different virulence factors and regulatory mechanisms, allowing it to adapt to hostile environments (3). Moreover, changing environmental conditions result in rapid diversification of P. aeruginosa genotype to produce a range of morphologically distinct phenotypic variants (4, 5) through processes such as phase variation (6) and adaptive mutations (7). This adaptability is believed to give the variants a selective advantage under unfavorable conditions. Clinical isolates of Pseudomonas have been reported to display a high degree of phenotypic diversity (8-10), especially those isolated from antibiotic treated patients. As a result of its high adaptability (11) and intrinsic antibiotic resistance (12), infections caused by P. aeruginosa are, in many instances, difficult to eradicate and can become persistent or even chronic (10), earning this bacterium a reputation as a paradigm of bacterial resistance (13).By selecting for phage resistance, bacteriophages have been identified as agents that can drive the emergence of P. aeruginosa variants due to the strong selective pressure that they exert on the host community (14, 15). At an estimated total of 10 31 , bacteriophages are the most abundant biological agent on earth (16) and, based on their prevalence and effects on prokaryotic communities, they can play a significan...

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Genotypic and phenotypic characterization of Pseudomonas aeruginosa isolates from urinary tract infections

Cited by 48 publications

References 79 publications

Virulence and Antibiotic Resistance of <b><i>Pseudomonas aeruginosa</i></b> Isolated from Patients with Urinary Tract Infections in Southern Poland

Virulence and Antibiotic Resistance of <b><i>Pseudomonas aeruginosa</i></b> Isolated from Patients with Urinary Tract Infections in Southern Poland

Metabolic profiles of household Pseudomonas species groups.

Predation in Homogeneous and Heterogeneous Phage Environments Affects Virulence Determinants of Pseudomonas aeruginosa

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