Genotypic and phenotypic characterization of rhizobia that nodulate snap bean (Phaseolus vulgaris L.) in Egyptian soils

Elbanna, Кhaled; Elbadry, M.; Gamal-El-Din, H.

doi:10.1016/j.syapm.2009.07.006

Cited by 24 publications

(17 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The amplitude of the genotypic variability found in this work agrees with the results observed in countries such as Brazil (Mostasso et al 2002;Hungria et al 2003;Cardoso et al 2009), Tunisia, and Egypt (Tamimi and Timko 2003;Mnasri et al 2007;Elbanna et al 2009), and also confirms evidences demonstrating that the potential for rhizobial symbiosis, nodulation, and nitrogen fixation is usually higher among local genotypes than among breeding lines (Martínez-Romero et al 1998;Provorov and Tikhonovich 2003;Kovas et al 2005). The superiority of certain local genotypes, and the correlation of responses between field and controlled conditions experiments, supports the hypothesis that local genotypes constitute a preferential material for the initial breeding of symbiotically active lines, although the cultivar-rhizobia and environment interaction could complicate the selection for improved Nitrogen fixation (Martensson and Rydberg 1996;Herridge and Rose 2000).…”

Section: Discussionsupporting

confidence: 91%

Variation for nodulation and plant yield of common bean genotypes and environmental effects on the genotype expression

Rodiño¹,

Fuente²,

Ron³

et al. 2011

Plant Soil

View full text Add to dashboard Cite

Common bean symbiotic nitrogen fixation provides an ecological and economical alternative to increase bean production but it depends on soil fertility and climate conditions. The objectives of this work were to characterize common bean genotypes for their ability to establish symbiosis under controlled conditions and to study the effect of the environment on the expression of those genotypes. The experiment under controlled conditions was conducted in a greenhouse with 158 genotypes that represented the major dry bean market classes. The field experiment was carried out in six environments with 64 genotypes that were previously selected for their contrasting nodulation ability and/or root development under the controlled conditions experiment. Nodulation, plant, and grain yield data of the bean genotypes were measured in both experiments. There was a significant high variability in plant development responses among the studied genotypes associated with the rhizobial strain inoculated under controlled conditions. Two nodulation phenotypes were observed among the genotypes tested: the big-nodules phenotype (BNO) associated with almost 63% fewer nodules and 58% higher proportion of nodule biomass in the below-ground compartment than the smallnodules phenotype (SNO) with less developed aerial parts. Genotype plus genotype × environment (GGE) biplot model analysis enabled identification of the highest-yielding genotypes for the different environments. The soil chemical factors of these environments were associated with the nodule number or the biomass of the common bean genotypes. Genotypes with a BNO phenotype showed a good plant response, indicating that this phenotype may be more beneficial for plant growth and seed yield in environmental conditions that may limit nodule development. The amplitude of the genotypic variability found in this work confirms the potential for rhizobial symbiosis of adapted bean genotypes, which could constitute a preferential material for initial breeding of symbiotically active lines. The data also indicate the potential of bean breeding to identify environments containing effective strains of rhizobia essential for sustainable agriculture, improving productivity, and maintaining environmental quality.

show abstract

Section: Discussionsupporting

confidence: 91%

Variation for nodulation and plant yield of common bean genotypes and environmental effects on the genotype expression

Rodiño¹,

Fuente²,

Ron³

et al. 2011

Plant Soil

View full text Add to dashboard Cite

show abstract

“…Ferreira et al (2000) and Elbanna et al (2009) (Table 1). Thus, it is noteworthy that these strains responded well even in soils with moderate acidity.…”

Section: ----------------------------------R$ -----------------------mentioning

confidence: 94%

Acid tolerant Rhizobium strains contribute to increasing the yield and profitability of common bean in tropical soils

Oliveira

Figueiredo

Soares

et al. 2017

J. Soil Sci. Plant Nutr.

View full text Add to dashboard Cite

Symbiosis of common bean with nitrogen-fixing bacteria can lead to a reduction in production costs and in environmental impacts. Considering the diversity of soils and climates, it is important to validate bacterial strains under different conditions to consolidate their recommendation as inoculants. Studies concerning the economic viability of Rhizobium inoculation in common bean are few, but they could assist in choosing the strain to be adopted. The aim of this study was to evaluate the performance of Rhizobium strains isolated from acid soils from the Amazon inoculated in seeds of the 'BRSMG Madrepérola' cultivar and to evaluate the economic viability of their use in the field. Four field experiments were conducted in the state of Minas Gerais, Brazil. A randomized block experimental design was used with four replications and seven treatments: inoculation with the strains CIAT 899 (Rhizobium tropici), UFLA 02-100 (R. etli), UFLA 02-68 (R. etli bv. mimosae), UFLA 02-127 (R. leguminosarum bv. phaseoli), and UFLA 04-173 (R. miluonense), plus two controls, one without inoculation + mineral nitrogen (N-urea) at a rate of 80 kg N ha -1 and another without inoculation and without mineral N. The strain CIAT 899 is already recommended for the manufacture of commercial inoculants, and the others, collected in the Amazon region, have proven to be highly effective in biological nitrogen fixation. Symbiosis of common bean with all the strains evaluated leads to satisfactory yields, with reduction in the application rate of mineral nitrogen and in environmental impacts and, consequently, reduction in economic costs, with higher profitability.

show abstract

“…These results indicate that the occurrence of B. altitudinis is not exclusive to high altitudes, but also occurs at ground level. However, when discriminating between closely related species of the same genus, DNA-DNA hybridization and housekeeping genes sequences should be the methods of choice, in accordance with the proposed molecular definition of species (Murray et al 1990;Berkum et al 1996;Elbanna et al 2009).…”

Section: Bacillus Amyloliquefaciens Strain Nbrc 15535 (Gi|343200768)mentioning

confidence: 99%

Characterization of Bacillus altitudinis as a New Causative Agent of Bacterial Soft Rot

Elbanna

Elnaggar

Bakeer

2014

Journal of Phytopathology

Self Cite

View full text Add to dashboard Cite

A total of 67 bacterial isolates were obtained from apple and pear fruits with signs of soft rot collected from Egyptian markets. Pathogenicity tests showed that 25 isolates (37%) were pathogenic to apple and pear fruits, with considerable variation of virulence. Among these isolates, 16 (64%) were Gram‐positive, motile, spore‐forming long rods and were identified as members of the genus Bacillus based on an API test. In addition, five isolates (20%) were Gram‐negative, non‐spore‐forming, motile, oxidase and catalase‐positive short rods and were identified as members of the genus Pseudomonas. Furthermore, four isolates (16%) were Gram‐negative, non‐spore‐forming, motile, catalase‐positive and oxidase negative short rods and were identified as belonging to the genus Erwinia. All selected isolates showed a wide host range and could cause soft rot of all representative fruits and vegetables tested. The three most virulent isolates, AB4, AB6 and PB6, exhibited the highest soft rot severity on different apple and pear cultivars, and apple cv. Anna (116) was the most susceptible to infection by isolates AB4 and AB6, with soft rot severities of 63.33 and 60.67%, respectively. Also, pear cv. Le‐Conte was most susceptible to infection by isolate AB6, with a soft rot severity of 89.9%. A phylogenetic tree based on 16S rRNA gene sequences indicated that strains AB4 and AB6 were very similar to one another and also showed a similarity of 99% to Bacillus altitudinis, and strain PB6 revealed a similarity of 99% to Bacillus pumilus. To our knowledge, this is the first report of B. altitudinis as a soft rot pathogen for both apple and pear fruits.

show abstract

Genotypic and phenotypic characterization of rhizobia that nodulate snap bean (Phaseolus vulgaris L.) in Egyptian soils

Cited by 24 publications

References 22 publications

Variation for nodulation and plant yield of common bean genotypes and environmental effects on the genotype expression

Variation for nodulation and plant yield of common bean genotypes and environmental effects on the genotype expression

Acid tolerant Rhizobium strains contribute to increasing the yield and profitability of common bean in tropical soils

Characterization of Bacillus altitudinis as a New Causative Agent of Bacterial Soft Rot

Contact Info

Product

Resources

About