2015
DOI: 10.3168/jds.2014-8044
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Genotypic and phenotypic characterization of Staphylococcus aureus causing persistent and nonpersistent subclinical bovine intramammary infections during lactation or the dry period

Abstract: Staphylococcus aureus is a significant pathogen frequently causing persistent intramammary infections (IMI) in dairy cows. We compared some genotypic and phenotypic characteristics of 285 strains collected from quarter milk samples from cows with persistent and nonpersistent subclinical IMI across Canada. Variable number of tandem repeats typing was used to infer the persistence of the same S. aureus strain in 3 consecutive quarter milk samples collected at intervals of 3 wk during lactation or before and afte… Show more

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Cited by 56 publications
(50 citation statements)
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“…For the immunological tests, we selected four different bovine mastitis isolates corresponding to some of the predominant S . aureus spa types found in Canadian dairy herds and elsewhere in the world [13,34]. Strain SHY97-3906 ( spa t529) was isolated from a case of clinical bovine mastitis that occurred during the lactation period, and CLJ08-3 ( spa t359) was originally isolated from a cow with persistent mastitis at dry-off [19].…”
Section: Methodsmentioning
confidence: 99%
See 2 more Smart Citations
“…For the immunological tests, we selected four different bovine mastitis isolates corresponding to some of the predominant S . aureus spa types found in Canadian dairy herds and elsewhere in the world [13,34]. Strain SHY97-3906 ( spa t529) was isolated from a case of clinical bovine mastitis that occurred during the lactation period, and CLJ08-3 ( spa t359) was originally isolated from a cow with persistent mastitis at dry-off [19].…”
Section: Methodsmentioning
confidence: 99%
“…The ability of S . aureus strains to produce biofilm in vitro was evaluated as described before [13]. Whenever required, ampicillin (100μg/ml) (Sigma-Aldrich, Oakville, ON, Canada), chloramphenicol (20 μg/ml) (ICN Biomedicals, Irvine, CA), and erythromycin (10 μg/ml) (Sigma) were added to culture media.…”
Section: Methodsmentioning
confidence: 99%
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“…Outros autores, como Veh et al (18) , caracterizaram S. aureus persistentes e não persistentes durante a lactação e o período seco de vacas e definiram um intervalo de tempo de no mínimo seis semanas para a classificação da persistência de estirpes estafilocócicas na glândula mamária, apesar do presente trabalho estar relacionado com espécie animal distinta.…”
Section: Zafalon Lf Et Alunclassified
“…Em vacas, amostras de S. aureus que persistiram no decorrer do período seco produziram significativamente mais biofilme in vitro que amostras que não persistiram após a parição, sendo possível que a terapia à secagem seja menos eficiente contra os micro-organismos com grande formação de biofilmes (18) . Estes mesmos autores citaram que S. aureus que persistem durante a lactação e no período seco são problemáticos porque permanecem como reservatórios para novas infecções dentro do rebanho.…”
Section: Zafalon Lf Et Alunclassified