Genotypic and Phenotypic Characterization of Soybean Sinorhizobial Strains Isolated from Brazilian Soils

Characterization and Effectiveness of Co‐inoculation of Sinorhizobium Strains on Annual Medics

“…A polyphasic approach based on a combination of all the information derived from phenotypic and genotypic data sets is most commonly used to characterize microorganisms (Diouf et al, 2000; Janecka et al, 2002). Phenotypic information is based on the morphology, growth rate (Mateos et al, 2002), utilization patterns of different carbohydrates and amino acids (Wolde‐meskel et al, 2004), protein profiling (Liu et al, 2011; Zahran et al, 2003), and fatty acid analysis (Tighe et al, 2000). Genetic information is derived from the complete genome such as polymerase chain reaction (PCR) based on repetitive sequences (rep‐PCR) (Bernal and Graham, 2001; Han et al, 2008; Montecchia et al, 2002; Muresu et al, 2005; Yan et al, 2007; Zhang et al, 2010) or analysis of targeted genes such as the RNA operon, rec A, atp D, and other housekeeping genes (Johnston et al, 2008; Katoh and Toh, 2010; Martir et al, 2007; Ramirez‐Bahena et al, 2009; Tian et al, 2011; Wu et al, 2011; Yokoyama et al, 2006).…”