Genotypic and phenotypic differentiation of an antifungal biocontrol strain belonging toBacillus subtilis

Marten, P.; Smalla, Kornelia; Berg, Gabriele

doi:10.1046/j.1365-2672.2000.01136.x

Cited by 67 publications

(46 citation statements)

References 41 publications

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“…Jedabi and Awatif (2009) have reported similar elevation in root colonization assay for different species of Bacillus after four days of germination, when compared to the control with 100 cfu/cm root length count. Several other research groups have reported similar results on lowered disease incitation and enhanced growth through heightened root colonization (Bais et al, 2004;Basha and Ulaganathan 2002;Marten et al, 2000). Thus, the study identifies the elevated root colonisation ability of B. subtilis better than the untreated control.…”

Section: Root Colonisation Assay (In Vitro)supporting

confidence: 69%

Biocontrol of Rhizoctonia solani Root Rot of Chilli by Bacillus subtilis formulations under Pot Conditions

Narasimhan

Shivakumar

2016

JBC

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Section: Root Colonisation Assay (In Vitro)supporting

confidence: 69%

Biocontrol of Rhizoctonia solani Root Rot of Chilli by Bacillus subtilis formulations under Pot Conditions

Narasimhan

Shivakumar

2016

JBC

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“…Recent studies deal with the genetic diversity of strains of B. subtilis (30,36,38,48,56), and the two closely related species B. licheniformis and B. pumilus have been placed in the B. subtilis group.…”

Section: Discussionmentioning

confidence: 99%

Genotyping and Toxigenic Potential ofBacillus subtilisandBacillus pumilusStrains Occurring in Industrial and Artisanal Cured Sausages

Matarante

Baruzzi

Cocconcelli

et al. 2004

Appl Environ Microbiol

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Artisanal and industrial sausages were analyzed for their aerobic, heat-resistant microflora to assess whether new emerging pathogens could be present among Bacillus strains naturally contaminating cured meat products. Sixty-four isolates were characterized by randomly amplified polymorphic DNA (RAPD)-PCR and fluorescent amplified fragment length polymorphism (fAFLP). The biotypes, identified by partial 16S rRNA gene sequence analysis, belonged to Bacillus subtilis, Bacillus pumilus, and Bacillus amyloliquefaciens species. Both RAPD-PCR and fAFLP analyses demonstrated that a high genetic heterogeneity is present in the B. subtilis group even in strains harvested from the same source, making it possible to isolate 56 different biotypes. Moreover, fAFLP analysis made it possible to distinguish B. subtilis from B. pumilus strains. The strains were characterized for their toxigenic potential by molecular, physiological, and immunological techniques. Specific PCR analyses revealed the absence of DNA sequences related to HBL, BcET, NHE, and entFM Bacillus cereus enterotoxins and the enzymes sphingomyelinase Sph and phospholipase PI-PLC in all strains; also, the immunological analyses showed that Bacillus strains did not react with NHE-and HBL-specific antibodies. However, some isolates were found to be positive for hemolytic and lecithinase activity. The absence of toxigenic potential in Bacillus strains from the sausages analyzed indicates that these products can be considered safe under the processing conditions they were produced; however, great care should be taken when the ripening time is shortened, particularly in the case of traditional sausages, which could contain high amounts of Bacillus strains and possibly some B. cereus cells.

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“…The qualitative assay for chitinase production was performed according to the method described by Marten et al (2000). The cultures of the Bacillus spp.…”

Section: Production Of Cell Wall Degrading Enzymementioning

confidence: 99%

Isolation, evaluation and characterization of Bacillus subtilis from cotton rhizospheric soil with biocontrol activity against Fusarium oxysporum

Gajbhiye

Alok

Meshram

et al. 2009

World J Microbiol Biotechnol

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Present investigation is based on the isolation of Bacillus subtilis from cotton rhizosphere and their evaluation as biocontrol agent against Fusarium oxysporum. The production of extracellular hydrolytic enzyme was studied for determining the antagonism. 43% of 21 isolates were identified under the B. subtilis group on the basis of biochemical characterization. 38% isolates showed competitive activity against Fusarium oxysporum and exhibit more than 50% mycelial inhibition in dual culture bioassay. The pot assay of cotton by seed treatment and soil amendment technique under green house condition showed the competent activity of the isolates in preventing the wilting of cotton seedlings due to F. oxysporum infection. SVI values of 30 day old seedlings indicated that the soil inoculation with B. subtilis BP-2 and seed treatment with B. subtilis BP-9 significantly promoted the growth of cotton seedlings. RAPD profiling revealed the diversity in the Bacillus subtilis group, ranging from 10 to 32%. The discriminative pattern among the isolates belonging to the same species was validated by 16S rDNA partial sequencing which identified them into four different strains of B. subtilis.

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Genotypic and phenotypic differentiation of an antifungal biocontrol strain belonging toBacillus subtilis

Cited by 67 publications

References 41 publications

Biocontrol of <I>Rhizoctonia solani</I> Root Rot of Chilli by <I>Bacillus subtilis</I> formulations under Pot Conditions

Biocontrol of <I>Rhizoctonia solani</I> Root Rot of Chilli by <I>Bacillus subtilis</I> formulations under Pot Conditions

Genotyping and Toxigenic Potential ofBacillus subtilisandBacillus pumilusStrains Occurring in Industrial and Artisanal Cured Sausages

Isolation, evaluation and characterization of Bacillus subtilis from cotton rhizospheric soil with biocontrol activity against Fusarium oxysporum

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