Genotypic and phenotypic diversity of Lactobacillus delbrueckii subsp. lactis strains of dairy origin

Giraffa, Giorgio; Andrighetto, Christian; Antonello, C; Gatti, Monica; Lazzi, Camilla; Marcazzan, Gian Luigi; Lombardi, Angiolella; Neviani, Erasmo

doi:10.1016/s0168-1605(03)00368-4

Cited by 54 publications

(35 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, several research groups have recognized that the identifications based on phenotypic and genotypic methods do not match (Giraffa et al, 2004;Miteva et al, 2001). Furthermore, although we had attempted to identify L. delbrueckii subspecies by using single housekeeping genes in the past, the resulting subspeciation was of low resolution (data not shown).…”

Section: Discussionmentioning

confidence: 97%

Multilocus sequence typing reveals a novel subspeciation of Lactobacillus delbrueckii

Tanigawa

Watanabe

2011

Microbiology

View full text Add to dashboard Cite

Currently, the species Lactobacillus delbrueckii is divided into four subspecies, L. delbrueckii subsp. delbrueckii, L. delbrueckii subsp. bulgaricus, L. delbrueckii subsp. indicus and L. delbrueckii subsp. lactis. These classifications were based mainly on phenotypic identification methods and few studies have used genotypic identification methods. As a result, these subspecies have not yet been reliably delineated. In this study, the four subspecies of L. delbrueckii were discriminated by phenotype and by genotypic identification [amplified-fragment length polymorphism (AFLP) and multilocus sequence typing (MLST)] methods. The MLST method developed here was based on the analysis of seven housekeeping genes (fusA, gyrB, hsp60, ileS, pyrG, recA and recG). The MLST method had good discriminatory ability: the 41 strains of L. delbrueckii examined were divided into 34 sequence types, with 29 sequence types represented by only a single strain. The sequence types were divided into eight groups. These groups could be discriminated as representing different subspecies. The results of the AFLP and MLST analyses were consistent. The type strain of L. delbrueckii subsp. delbrueckii, YIT 0080 T , was clearly discriminated from the other strains currently classified as members of this subspecies, which were located close to strains of L. delbrueckii subsp. lactis. The MLST scheme developed in this study should be a useful tool for the identification of strains of L. delbrueckii to the subspecies level.

show abstract

Section: Discussionmentioning

confidence: 97%

Multilocus sequence typing reveals a novel subspeciation of Lactobacillus delbrueckii

Tanigawa

Watanabe

2011

Microbiology

View full text Add to dashboard Cite

show abstract

“…Natural isolates usually exhibit phenotypic variability with minor or major changes in phenotype and genotype due to different environmental pressures [32,33]. Although the obtained results indicate that it is not possible to establish one specific phenotypic feature by which the strains can be differentiated with respect to their source, nisin resistance could be potentially linked to regions 10, 11, and 19 ( Fig.…”

Section: Discussionmentioning

confidence: 94%

Molecular diversity among natural populations of Lactobacillus paracasei and Lactobacillus plantarum/paraplantarum strains isolated from autochthonous dairy products

Golić

Strahinić

Terzić-Vidojević

et al. 2012

Eur Food Res Technol

View full text Add to dashboard Cite

The diversity of 87 Lactobacillus paracasei and Lactobacillus plantarum/paraplantarum strains, previously identified from different autochthonous dairy products, was investigated by phenotypic and genotypic approaches. The increased resolution obtained using phenotypic and genotypic characterization allowed the level of strain heterogeneity detection to be widened. Phenotypic diversity was evaluated by studying biochemical characteristics of technological interest, including antimicrobial and proteinase activities, resistance to nisin, aggregation ability, production of exopolysaccharides, acetoin and diacetyl, citrate utilization, and antibiotic susceptibility. Genotypic diversity was generally evaluated by PCR amplification of repetitive bacterial DNA element fingerprinting using the (GTG) 5 primer [(GTG) 5 -PCR]. Moreover, in cases where strains were not discriminated by (GTG) 5 -PCR combined with phenotypic analysis, pulsedfield gel electrophoresis (PFGE) analysis was performed.The results indicate that L. plantarum/paraplantarum and L. paracasei natural isolates from artisanal dairy products are a gold mine in terms of diversity of strains and could be potentially interesting to dairy companies for the formulation of functional starter cultures in the production of innovative foods.

show abstract

“…Distinguishing between L. delbrueckii subspecies either by phenotypic or genetic methods is not without its difficulties [14,15]. Their ARDRA profiles are very similar (if not identical) with most restriction enzymes [15], and although subspecies-specific nucleotide signatures in the 16S rRNA gene sequences have been proposed [14], these differences may be too small to allow for any accurate identification.…”

Section: Discussionmentioning

confidence: 99%

“…Acetaldehyde was the major compound in all fermented milk samples, having a relative concentration (6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17) and L. helveticus (18)(19). M molecular weight marker between 43 and 93 % of the total volatiles.…”

Section: Characterization Of the Thermophilic Labmentioning

confidence: 99%

Identification, typing and functional characterization of dominant lactic acid bacteria strains from Iranian traditional yoghurt

Hajimohammadi-Farimani

Najafi

Bazzaz

et al. 2015

Eur Food Res Technol

View full text Add to dashboard Cite

Genotypic and phenotypic diversity of Lactobacillus delbrueckii subsp. lactis strains of dairy origin

Cited by 54 publications

References 23 publications

Multilocus sequence typing reveals a novel subspeciation of Lactobacillus delbrueckii

Multilocus sequence typing reveals a novel subspeciation of Lactobacillus delbrueckii

Molecular diversity among natural populations of Lactobacillus paracasei and Lactobacillus plantarum/paraplantarum strains isolated from autochthonous dairy products

Identification, typing and functional characterization of dominant lactic acid bacteria strains from Iranian traditional yoghurt

Contact Info

Product

Resources

About