Genotypic and phenotypic properties of coagulase-negative staphylococci causing dialysis catheter-related sepsis

Spare, M.K.; Tebbs, S. E.; Lang, Susan; Lambert, Peter A.; Worthington, Tony; Lipkin, Graham; Elliott, Tom

doi:10.1016/s0195-6701(03)00154-3

Cited by 28 publications

(20 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This made it possible to investigate the establishment of the colonizing CoNS microflora in EPT newborns with a higher discriminatory power than most earlier methods could offer (15,16). In two-thirds of the investigated preterm newborns, colonizing CoNS strains that persisted during the first postnatal weeks were already retrieved on postnatal day 2-3.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Colonization pattern of coagulase-negative staphylococci in preterm neonates and the relation to bacteremia

Björkqvist

Liljedahl

Zimmermann

et al. 2010

Eur J Clin Microbiol Infect Dis

View full text Add to dashboard Cite

International audienceCoagulase-negative staphylococci (CoNS) are the major cause of sepsis in extreme preterm (EPT) newborns, but data on the CoNS colonization in EPT newborns prior to invasive infection are limited. Our aim was to describe the early establishment of the CoNS microflora in EPT newborns and to compare the colonization pattern in neonates with and without positive CoNS blood cultures. From a cohort of 46 EPT neonates, newborns with positive CoNS blood culture were identified ( = 10) and compared with matched controls. Samples for bacterial cultures were obtained repetitively from nares, perineum, and umbilicus. All CoNS isolates were characterized using the PhenePlate system for biochemical fingerprinting. Persistent CoNS strains were found on day 2-3 after delivery in 7/20 newborns, and there was a tendency for earlier colonization in nares than in the perineum or umbilicus. The CoNS blood strains were prevalent in superficial sites prior to positive blood culture (11/14 blood strains), but no single invasive pathway was identified. Most CoNS blood strains (9/14) persisted on superficial sites after antibiotic treatment. We hypothesize that the invasive pathways in neonatal CoNS sepsis are complex and that the colonization of mucosal membranes and umbilical catheters might be of equal importance

show abstract

Section: Discussionmentioning

confidence: 99%

“…Pulsed field gel electrophoresis (PFGE) and other conventional genetic typing methods are laborious and not applicable to the large populations of bacterial isolates found in superficial samples, whereas earlier phenotypic typing methods do not show appropriate discriminatory power (15,16).…”

mentioning

confidence: 99%

Colonization pattern of coagulase-negative staphylococci in preterm neonates and the relation to bacteremia

Björkqvist

Liljedahl

Zimmermann

et al. 2010

Eur J Clin Microbiol Infect Dis

View full text Add to dashboard Cite

show abstract

“…However, this will result in a delay of at least 2 days, compared to the typing of isolates after a 20-h incubation time, as we have used here. This is not a problem in a research setting but could be a consideration in a clinical situation, e.g., for the microbiological diagnosis of catheter-related bloodstream infections (CR-BSI) (5,16). Furthermore, previous studies indicated that polyclonality in CNS cultures from CR-BSI isolates correlates with differences in antimicrobial susceptibility patterns and may have important consequences for the treatment of these infections (5).…”

Section: Discussionmentioning

confidence: 99%

“…Bloodstream infections (BSI) caused by these organisms are frequently related to the use of medical devices. They account for significant morbidity and mortality, especially in immunocompromised individuals and neonates (16,17,20).…”

mentioning

confidence: 99%

Proof of Principle for Successful Characterization of Methicillin-Resistant Coagulase-Negative Staphylococci Isolated from Skin by Use of Raman Spectroscopy and Pulsed-Field Gel Electrophoresis

Willemse-Erix

Jachtenberg²,

Barutçi

et al. 2010

J Clin Microbiol

View full text Add to dashboard Cite

Coagulase-negative staphylococci (CNS) are among the most frequently isolated bacterial species in clinical microbiology, and most CNS-related infections are hospital acquired. Distinguishing between these frequently multiple-antibiotic-resistant isolates is important for both treatment and transmission control. In this study we used isolates of methicillin-resistant coagulase-negative staphylococci (MR-CNS) that were selected from a large surveillance study of the direct spread of MR-CNS. This strain collection was used to evaluate (i) Raman spectroscopy as a typing tool for MR-CNS isolates and (ii) diversity between colonies with identical and different morphologies. Reproducibility was high, with 215 of 216 (99.5%) of the replicate samples for 72 isolates ending up in the same cluster. The concordance with pulsed-field gel electrophoresis (PFGE)-based clusters was 94.4%. We also confirm that the skin of patients can be colonized with multiple MR-CNS types at the same time. Morphological differences between colonies from a single patient sample correlated with differences in Raman and PFGE types. Some morphologically indistinguishable colonies revealed different Raman and PFGE types. This indicates that multiple MR-CNS colonies should be examined to obtain a complete insight into the prevalence of different types and to be able to perform an accurate transmission analysis. Here we show that Raman spectroscopy is a reproducible typing system for MR-CNS isolates. It is a tool for screening variability within a collection of isolates. Because of the high throughput, it enables the analysis of multiple colonies per patient, which will enhance the quality of clinical and epidemiological studies.

show abstract

“…In sophisticated laboratory settings, strain identity can be confirmed by DNA genotype analysis (235).…”

Section: Guideline 15 -Relapsing Peritonitismentioning

confidence: 99%

Consensus Guidelines for the Prevention and Treatment of Catheter-Related Infections and Peritonitis in Pediatric Patients Receiving Peritoneal Dialysis: 2012 Update

et al. 2012

View full text Add to dashboard Cite

Genotypic and phenotypic properties of coagulase-negative staphylococci causing dialysis catheter-related sepsis

Cited by 28 publications

References 25 publications

Colonization pattern of coagulase-negative staphylococci in preterm neonates and the relation to bacteremia

Colonization pattern of coagulase-negative staphylococci in preterm neonates and the relation to bacteremia

Proof of Principle for Successful Characterization of Methicillin-Resistant Coagulase-Negative Staphylococci Isolated from Skin by Use of Raman Spectroscopy and Pulsed-Field Gel Electrophoresis

Consensus Guidelines for the Prevention and Treatment of Catheter-Related Infections and Peritonitis in Pediatric Patients Receiving Peritoneal Dialysis: 2012 Update

Contact Info

Product

Resources

About