Genotypic and Phenotypic Trends in Antibiotic Resistant Pathogenic Escherichia coli Isolated from Humans and Farm Animals in South Korea

Unno, Tatsuya; Han, Dukki; Jang, Jeonghwan; Widmer, Kenneth W.; Ko, GwangPyo; Sadowsky, Michael J.; Hur, Hor‐Gil

doi:10.1264/jsme2.me10194

Cited by 15 publications

(10 citation statements)

References 31 publications

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“…Resistance to streptomycin, ampicillin, tetracycline, chloramphenicol and trimethoprim-sulfamethoxazole has been observed in other studies of dog fecal isolates 18 , 19 , 20 , 21 , 23 , 25 and human fecal isolates. 26 , 27 Aminopenicillins and aminoglycosides appear most often among the three classes of drugs with high frequencies of resistance in studies of samples of animal origin. 6 , 21 , 23 , 28 , 29 , 30 , 31 , 32 , 33 , 34 Strains resistant to the carbapenems tested in this study were not detected.…”

Section: Resultsmentioning

confidence: 99%

Resistance patterns, ESBL genes, and genetic relatedness of Escherichia coli from dogs and owners

Carvalho

Barbosa

Arais

et al. 2016

Brazilian Journal of Microbiology

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Antimicrobial resistance in Escherichia coli isolated from pet dogs can be considered a potential threat of infection for the human population. Our objective was to characterize the resistance pattern, extended spectrum beta-lactamase production and genetic relatedness of multiresistant E. coli strains isolated from dogs (n = 134), their owners (n = 134), and humans who claim to have no contact with dogs (n = 44, control), searching for sharing of strains. The strains were assessed for their genetic relatedness by phylogenetic grouping and pulsed-field gel electrophoresis. Multiresistant E. coli strains were isolated from 42 (31.3%) fecal samples from pairs of dogs and owners, totaling 84 isolates, and from 19 (43.1%) control group subjects. The strains showed high levels of resistance to ampicillin, streptomycin, tetracycline, trimethoprim and sulfamethoxazole regardless of host species or group of origin. The blaTEM, blaCTX-M, and blaSHV genes were detected in similar proportions in all groups. All isolates positive for bla genes were ESBL producers. The phylogenetic group A was the most prevalent, irrespective of the host species. None of the strains belonging to the B2 group contained bla genes. Similar resistance patterns were found for strains from dogs, owners and controls; furthermore, identical PFGE profiles were detected in four (9.5%) isolate pairs from dogs and owners, denoting the sharing of strains. Pet dogs were shown to be a potential household source of multiresistant E. coli strains.

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Section: Resultsmentioning

confidence: 99%

Resistance patterns, ESBL genes, and genetic relatedness of Escherichia coli from dogs and owners

Carvalho

Barbosa

Arais

et al. 2016

Brazilian Journal of Microbiology

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“…Only 1 strain was resistant to at least 3 classes of antimicrobials. In contrast, multidrug resistance was reported among DEC isolates in Iran, Kenia, Mexico, and South Korea (Al Jarousha et al, 2011; Harel et al, 1991; Sang et al, 2012; Unno et al, 2011). …”

Section: Discussionmentioning

confidence: 99%

Enteropathogenic and enteroaggregative E. coli in stools of children with acute gastroenteritis in Davidson County, Tennessee

Foster

Iqbal

Zhang

et al. 2015

Diagnostic Microbiology and Infectious Disease

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This prospective acute gastroenteritis (AGE) surveillance was conducted in the inpatient and emergency room settings at a referral pediatric hospital to determine the prevalence of diarrheagenic Escherichia coli (DEC) in children<12 years of age with AGE in Davidson County, Tennessee. Subjects 15 days to 11 years of age, who presented with diarrhea and/or vomiting, were enrolled. Stool specimens were processed for detection of DEC using multiplex polymerase chain reaction. From December 1, 2011, to June 30, 2012, a total of 79 (38%) out of 206 stool specimens from children with AGE tested positive for E. coli. A total of 12 (5.8%) out of 206 stool specimens from children with AGE were positive for a DEC. Eight (67%) out of these 12 were positive for enteropathogenic E. coli, and the remaining 4 were positive for enteroaggregative E. coli. DEC clinical isolates clustered with known E. coli enteropathogens according to multilocus sequencing typing.

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“…E. coli strains of each pathotype show variation in the distribution of phylogenetic groups due to virulence characteristics. Variation has also been recorded in the phylogenetic grouping of ExPEC strains of poultry origin (27).…”

Section: Discussionmentioning

confidence: 99%

Phylogrouping and antimicrobial resistance analysis of extraintestinal pathogenic Escherichia coli isolated from poultry species

Bhave¹,

Kolhe²,

MAHADEVASWAMY³

et al. 2019

Turk J Vet Anim Sci

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This study was undertaken to determine the prevalence and antimicrobial resistance pattern of extraintestinal pathogenic Escherichia coli (ExPEC) in the feces of healthy poultry and retail chicken. All 146 E. coli recovered from 351 samples were screened by PCR for detection of ExPEC strains. Nineteen (13.01%) isolates were confirmed as ExPEC. Distribution of ExPEC strains was revealed as follows: broiler (25%), layer (15.87%), and raw chicken (3.12%). Turkey, duck, and water samples were negative for ExPEC strains. ExPEC strains belonged to phylogenetic groups B2 (52.63%), A (36.84%), and D (10.53%). Twenty-three (15.75%) isolates were ESBLpositive, including four ExPEC strains. ESBL-positive E. coli were isolated from all the samples except turkey. A high degree of resistance to commonly used antimicrobials, namely nalidixic acid (95.89%), tetracycline (95.89%), trimethoprim (89.04%), colistin (82.88%), and ciprofloxacin (54.11%), including β-lactam antimicrobials ampicillin (84.93%) and amoxicillin/clavulanic acid (81.51%), was expressed by the isolates. Out of fifteen randomly selected ESBL-positive E. coli isolates, β-lactam genes, namely blaTEM, blaCTX-M, and blaOXA, were detected in three, six, and one, respectively. Poultry and raw chicken harbor multidrug-resistant, ESBL-type E. coli as well as ExPEC and these strains may be transmitted to humans via the food chain.

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Genotypic and Phenotypic Trends in Antibiotic Resistant Pathogenic Escherichia coli Isolated from Humans and Farm Animals in South Korea

Cited by 15 publications

References 31 publications

Resistance patterns, ESBL genes, and genetic relatedness of Escherichia coli from dogs and owners

Resistance patterns, ESBL genes, and genetic relatedness of Escherichia coli from dogs and owners

Enteropathogenic and enteroaggregative E. coli in stools of children with acute gastroenteritis in Davidson County, Tennessee

Phylogrouping and antimicrobial resistance analysis of extraintestinal pathogenic Escherichia coli isolated from poultry species

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