Genotypic characteristics and biofilm formation among Escherichia coli isolates from Indian women with acute cystitis

Agarwal, Jyotsna; Mishra, Bharti; Srivastava, Shubhi; Srivastava, Richa

doi:10.1093/trstmh/trs090

Cited by 19 publications

(13 citation statements)

References 16 publications

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“…It is believed that hly is more common among invasive uropathogenic strains than in nonpathogenic or commensal isolates of E. coli (38). In the present study, 77% of the hly genes were detected in phylogroups B 2 and D. This confirms the results of previous research showing that these phylogroups are more pathogenic and have more virulence factors than others, although different studies have shown the prevalence of hly in UPEC isolates to range from 2% to 47% in different countries (10,39,40).…”

Section: Discussionsupporting

confidence: 92%

Phylogenetic Grouping and Assessment of Virulence Genotypes, With Antibiotic Resistance Patterns, of Escherichia Coli Strains Implicated in Female Urinary Tract Infections

Staji

Khoshgoftar²,

Vayeghan³

et al. 2016

Int J Enteric Pathog

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Background: Escherichia coli strains are common pathogens that can cause urinary tract infections (UTIs). They are classified into phylogroups based on three genetic markers: chuA, yjaA, and TspE4.C2. The E. coli strains that cause UTIs possess several genes that encode urovirulent factors and antimicrobial-resistance phenotypes. We determined the phylogenetic groups of E. coli isolates from UTI cases in Sabzevar, Iran, the prevalence of certain virulence genes, and the antibiotic-resistance phenotypes in these strains. Objectives: The aim of this study was to assess the correlation of detected E. coli phylogroups in female UTI patients with the antibioticresistance pattern and the distribution of certain virulence factors among the phylogroups. Materials and Methods: Ninety-three E. coli isolates from 150 women with UTI were studied. Three genetic markers were detected for phylogenetic grouping of strains, and four virulence determinants were analyzed with multiplex-PCR, including the genes for hemolysin (hly), aerobactin (iucD), P fimbriae (pap), and S/F1C fimbriae (sfa/focDE). The antibiotic-resistance phenotypes were also determined. Results: The isolates from UTI cases were distributed within phylogroups A (31%), B 1 (10%), B 2 (28%), and D (31%). The prevalence of iucD, hly, pap, and sfa/focDE virulence genes was significantly associated with groups B 2 and D. The most-resisted antibiotics were cefazolin (93%) and co-trimoxazole (68%), while the isolates were most sensitive to nitrofurantoin (1%) and imipenem (2%). Conclusions: The phylogroups of E. coli isolates from UTI cases showed that groups D, B 2 , and A are prevalent in women in Sabzevar, as the dominant pathogenic phylogroups. The comparison showed that there was no significant difference in the occurrence of virulence factors or in the distribution of antibiotic resistance between urinary E. coli isolates, but the virulence genes were distributed more into groups B 2 and D, respectively. Our study showed that the highest sensitivity was to nitrofurantoin and imipenem, but the decision on a treatment strategy remains based on the physician's diagnosis and the antimicrobial-resistance tests.

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Section: Discussionsupporting

confidence: 92%

Phylogenetic Grouping and Assessment of Virulence Genotypes, With Antibiotic Resistance Patterns, of Escherichia Coli Strains Implicated in Female Urinary Tract Infections

Staji

Khoshgoftar²,

Vayeghan³

et al. 2016

Int J Enteric Pathog

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“…Biofilm production allows bacteria to persist in the host and can be associated with the gravity of the infection (Høiby et al, 2011 ). UPEC strains have been shown to present varying levels of biofilm production (Watts et al, 2010 ; Novais et al, 2012 ; Ponnusamy et al, 2012 ; Agarwal et al, 2013 ; Tapiainen et al, 2014 ; Flament-Simon et al, 2019 ). Herein, we showed that UPEC 252 is not capable of producing biofilm on abiotic surfaces under the conditions tested.…”

Section: Discussionmentioning

confidence: 99%

Genetic and Virulence Characteristics of a Hybrid Atypical Enteropathogenic and Uropathogenic Escherichia coli (aEPEC/UPEC) Strain

Valiatti

Santos

et al. 2020

Front. Cell. Infect. Microbiol.

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Hybrid strains of Escherichia coli combine virulence traits of diarrheagenic (DEC) and extraintestinal pathogenic E. coli (ExPEC), but it is poorly understood whether these combined features improve the virulence potential of such strains. We have previously identified a uropathogenic E. coli (UPEC) strain (UPEC 252) harboring the eae gene that encodes the adhesin intimin and is located in the locus of enterocyte effacement (LEE) pathogenicity island. The LEE-encoded proteins allow enteropathogenic E. coli (EPEC) and enterohemorrhagic E. coli (EHEC) to form attaching and effacing (A/E) lesions in enterocytes. We sought to characterize UPEC 252 through whole-genome sequencing and phenotypic virulence assays. Genome analysis unveiled that this strain harbors a complete LEE region, with more than 97% of identity comparing to E2348/69 (EPEC) and O157:H7 Sakai (EHEC) prototype strains, which was functional, since UPEC 252 expressed the LEE-encoded proteins EspB and intimin and induced actin accumulation foci in HeLa cells. Phylogenetic analysis performed comparing 1,000 single-copy shared genes clustered UPEC 252 with atypical EPEC strains that belong to the sequence type 10, phylogroup A. Additionally, UPEC 252 was resistant to the bactericidal power of human serum and colonized cells of the urinary (T24 and HEK293-T) and intestinal (Caco-2 and LS174T) tracts. Our findings suggest that UPEC 252 is an atypical EPEC strain that emerges as a hybrid strain (aEPEC/UPEC), which could colonize new niches and potentially cause intestinal and extraintestinal infections.

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“…In addition, the PapG II variant gene from MDR- and XDR-UPEC strains associated with phylogenetic groups D and B2 was the most prevalent compared to the papG I and papG III variant genes. The papG II variant gene from UPEC strains associated with phylogenetic groups A and B2 is the most prevalent gene associated with cystitis in women and pyelonephritis in children and adults (Jantunen et al, 2000; Agarwal et al, 2013). However, four MDR-UPEC strains associated with phylogenetic group B2 and one strain associated with group D were associated with the papG III variant gene, which correlates with data reported by other authors (Agarwal et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Multidrug- and Extensively Drug-Resistant Uropathogenic Escherichia coli Clinical Strains: Phylogenetic Groups Widely Associated with Integrons Maintain High Genetic Diversity

et al. 2016

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In recent years, an increase of uropathogenic Escherichia coli (UPEC) strains with Multidrug-resistant (MDR) and Extensively Drug-resistant (XDR) profiles that complicate therapy for urinary tract infections (UTIs) has been observed and has directly impacted costs and extended hospital stays. The aim of this study was to determine MDR- and XDR-UPEC clinical strains, their virulence genes, their phylogenetic groups and to ascertain their relationship with integrons and genetic diversity. From a collection of 500 UPEC strains, 103 were selected with MDR and XDR characteristics. MDR-UPEC strains were mainly associated with phylogenetic groups D (54.87%) and B2 (39.02%) with a high percentage (≥70%) of several fimbrial genes (ecpA, fimH, csgA, and papGII), an iron uptake gene (chuA), and a toxin gene (hlyA). In addition, a moderate frequency (40–70%) of other genes (iutD, tosA, and bcsA) was observed. XDR-UPEC strains were predominantly associated with phylogenetic groups B2 (47.61%) and D (42.85%), which grouped with ≥80 virulence genes, including ecpA, fimH, csgA, papGII, iutD, and chuA. A moderate frequency (40–70%) of the tosA and hlyA genes was observed. The class 1 and 2 integrons that were identified in the MDR- and XDR-UPEC strains were associated with phylogenetic groups D, B2, and A, while the XDR-UPEC strains that were associated with phylogenetic groups B2, D, and A showed an extended-spectrum beta-lactamase (ESBL) phenotype. The modifying enzymes (aadA1, aadB, aacC, ant1, dfrA1, dfrA17, and aadA4) that were identified in the variable region of class 1 and 2 integrons from the MDR strains showed resistance to gentamycin (56.25 and 66.66%, respectively) and trimethoprim-sulfamethoxazole (84.61 and 66.66%, respectively). The MDR- and XDR-UPEC strains were distributed into seven clusters and were closely related to phylogenic groups B2 and D. The diversity analysis by PFGE showed 42.68% of clones of MDR-UPEC and no clonal association in the XDR-UPEC strains. In conclusion, phylogenetic groups including virulence genes are widely associated with two integron classes (1 and 2) in MDR- and XDR-UPEC strains.

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Genotypic characteristics and biofilm formation among Escherichia coli isolates from Indian women with acute cystitis

Abstract: Distribution of virulence genotypes and phylotypes can vary with geographical location and the isolates from western countries cannot be treated as prototypes for Asian cystitis isolates.

Cited by 19 publications

References 16 publications

Phylogenetic Grouping and Assessment of Virulence Genotypes, With Antibiotic Resistance Patterns, of Escherichia Coli Strains Implicated in Female Urinary Tract Infections

Phylogenetic Grouping and Assessment of Virulence Genotypes, With Antibiotic Resistance Patterns, of Escherichia Coli Strains Implicated in Female Urinary Tract Infections

Genetic and Virulence Characteristics of a Hybrid Atypical Enteropathogenic and Uropathogenic Escherichia coli (aEPEC/UPEC) Strain

Multidrug- and Extensively Drug-Resistant Uropathogenic Escherichia coli Clinical Strains: Phylogenetic Groups Widely Associated with Integrons Maintain High Genetic Diversity

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