Genotypic characterization and clonal relatedness of metallo-β-lactamase-producing non-fermentative gram negative bacteria in the first 5 years of their circulation in Paraguay (2011-2015)

Franco, Rossana; Santos, Ivson Cassiano de Oliveira; Mora, Mario Martínez; López, Patricia; Takahasi, Vivian; Arce, Flavia Helena Ortiz; Lird, Graciela; Silvagni, Marlene; Kawabata, Akiko; Rojas, Carolina; Fernández, Mirna Fabiola Agüero; Oliveira, Thamirys Rachel Tavares E; Rocha-de-Souza, Cláudio Marcos; Carvalho-Assef, Ana Paula D’Alincourt

doi:10.1007/s42770-022-00888-x

Cited by 3 publications

(1 citation statement)

References 40 publications

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“…Regarding AST, Kirby-Bauer disk diffusion (n = 11, 65%) was most commonly used, followed by automated systems (n = 6, 35%), broth microdilution (n = 3, 18%), and E-test (n = 2, 12%). Automated systems included the VITEK (n = 5), BD-Phoenix (n = 1), and Sensititre (ThermoFisher) (n = 1) [ 16 , 17 , 29 , 32 – 34 ]. A variety of additional culture-based methods for carbapenemase detection were used in 10 studies (59%), with the double-disk synergy test (DDST) (n = 4) most commonly described.…”

Section: Resultsmentioning

confidence: 99%

In search of the best method to detect carriage of carbapenem-resistant Pseudomonas aeruginosa in humans: a systematic review

Shahab,

van Veen,

Büchler

et al. 2024

Ann Clin Microbiol Antimicrob

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Background Detection of carbapenem-resistant Pseudomonas aeruginosa (CR-PA) in humans is important to prevent transmission. However, the most optimal culture method to detect CR-PA is unknown. This systematic review aims to determine which culture method is most sensitive and which culture methods are used to detect CR-PA in humans. Second, to establish the most feasible culture method taking into account the turnaround time (TAT), and third, to provide an overview of the sampling sites used to detect carriage. Methods We systematically searched the electronic databases Embase, Medline Ovid, Cochrane, Scopus, CINAHL, and Web of Science until January 27, 2023. All diagnostic accuracy studies comparing two or more culture methods to detect CR-PA and recent outbreak or surveillance reports on CR-PA carriage or infection in humans, which describe culture methods and their results, were eligible for inclusion. We used QUADAS-2 guideline for diagnostic accuracy studies and the STROBE or ORION guideline for outbreak-surveillance studies to assess the risk of bias. Results Six diagnostic accuracy studies were included. An enrichment broth was found to increase the detection of CR-PA. Using an enrichment broth extended the TAT by 18–24 h, yet selective media could reduce the TAT by 24 h compared to routine media. In total, 124 outbreak-surveillance studies were included, of which 17 studies with surveillance samples and 116 studies with clinical samples. In outbreak-surveillance studies with surveillance samples, perianal, rectal swabs or stools were the most common sampling site/specimen (13/17, 76%). A large variety was observed in whether and which kind of enrichment broth and selective media were used. Conclusions We found a benefit of using an enrichment step prior to inoculation of the material onto selective media for the detection of CR-PA. More research is needed to determine the most sensitive sampling site and culture method. Trail registration: This study was registered in the PROSPERO International prospective register of systematic reviews (registration number: CRD42020207390, http://www.crd.york.ac.uk/PROSPERO/display_record.asp?ID=CRD42020207390).

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Section: Resultsmentioning

confidence: 99%

In search of the best method to detect carriage of carbapenem-resistant Pseudomonas aeruginosa in humans: a systematic review

Shahab,

van Veen,

Büchler

et al. 2024

Ann Clin Microbiol Antimicrob

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Genomic features and comparative analysis of a multidrug-resistant Acinetobacter bereziniae strain infecting an animal: a novel emerging one health pathogen?

Stehling,

Sellera,

de Almeida

et al. 2024

World J Microbiol Biotechnol

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Prevalence of colistin resistance in clinical isolates of Pseudomonas aeruginosa: a systematic review and meta-analysis

Narimisa,

Keshtkar,

Dadgar-Zankbar

et al. 2024

Front. Microbiol.

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ObjectiveThe emergence of resistance to colistin, the last resort for treating severe infections caused by Pseudomonas aeruginosa, poses a significant threat to public health. This meta-analysis aimed to investigate the prevalence of colistin resistance in clinical isolates of P. aeruginosa.MethodA comprehensive search of MEDLINE (PubMed), Web of Science, and Scopus databases was conducted to identify relevant articles published until December 2023. Subsequently, a meta-analysis was performed using Stata software to examine the pooled prevalence of colistin resistance and to conduct subgroup analyses.ResultsA total of 619 studies were included in the meta-analysis, revealing a global prevalence of colistin resistance of 1% among all P. aeruginosa isolates. Furthermore, cystic fibrosis patients exhibited the highest resistance to colistin, with a prevalence of 7% among the examined diseases.ConclusionThe increase in colistin resistance in P. aeruginosa in recent years from 2% (in the period of 2006–2010) to 5% (in the period of 2020–2023) underscores the need for implementing infection prevention programs, using appropriate treatment regimens, and disseminating comprehensive information on antimicrobial resistance patterns. These measures are crucial for addressing this growing public health concern.

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Genotypic characterization and clonal relatedness of metallo-β-lactamase-producing non-fermentative gram negative bacteria in the first 5 years of their circulation in Paraguay (2011-2015)

Cited by 3 publications

References 40 publications

In search of the best method to detect carriage of carbapenem-resistant Pseudomonas aeruginosa in humans: a systematic review

In search of the best method to detect carriage of carbapenem-resistant Pseudomonas aeruginosa in humans: a systematic review

Genomic features and comparative analysis of a multidrug-resistant Acinetobacter bereziniae strain infecting an animal: a novel emerging one health pathogen?

Prevalence of colistin resistance in clinical isolates of Pseudomonas aeruginosa: a systematic review and meta-analysis

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