Genotypic characterization by polymerase chain reaction of Staphylococcus aureus isolates associated with bovine mastitis

Characterization of virulence and antibiotic profile and agr typing of Staphylococcus aureus from milk of subclinical mastitis bovine in State of Rio de Janeiro

“…In this study, most S. aureus strains presented the genetic ability to produce biofilm since the slime production associated icaA and icaD genes were detected in 67.3% (37/55) and 87.3% (48/55), respectively. In Belgium, Ote et al (2011) detected 86.9% icaA and 95% icaB positive S. aureus strains in a 229 bovine mastitis sampling corroborating the present data of high prevalence of these genes in dairy environment. Presence of at least one of genes has been detected in most isolates of S. aureus bovine mastitis demonstrating its importance as virulence factors in the pathogenesis of bovine mastitis (Atkin et al, 2014).…”

A 3-year long study of Staphylococcus aureus isolates from subclinical mastitis in three Azawak zebu herds at the Sahelian experimental farm of Toukounous, Niger

“…Finally, the frequency of the icaA gene in S. aureus isolated from bovine mastitis can be highly different between studies: 15 % (Darwish and Asfour 2013), 58 % (Chavhan et al 2012), 86 % (Ote et al 2011), 98 % (Castelani et al 2015 vs. 76 % in the present study. Biofilm production by the 57 icaA-positive isolates was strong for 8 isolates (belonging to different pulsotypes), moderate for 31 isolates (including most A and B pulsotype isolates) but weak for 18 isolates (including all 8 D pulsotype isolates) (Table 6), emphasizing not only that the icaA gene may not be expressed by one third of the positive isolates in this in vitro assay but also that this lack of expression could be related to the pulsotype.…”

“…less than one third of them, similarly to other published results. The most noticeable difference is the much higher prevalence of the lukS-PV (39 %) and lukF-PV (31 %) genes coding for the Panton-Valentine leukocidin (PVL) compared to the results of other studies in Europe and Africa (Rainard et al 2003;Fueyo et al 2005;Karahan et al 2009;Ikawaty et al 2010;Kadja et al 2010;Ote et al 2011;Issa et al 2013). Since the PVL toxin is strongly associated with human S. aureus isolates causing skin and soft-tissue infections (Shallcross et al 2013), the lukS-PV-and/or lukF-PV-positive isolates in Toukounous might therefore be of human rather than of cattle origin, transferred during the manual milking process.…”

“…Each year, the S. aureus isolates were tested by PCR as described by Ote et al (2011) for the presence of genes coding for MSCRAMMs (clfA, clfB, fnbA, cna, ebpS, sdrC), biofilm formation (icaA), capsular antigens (cap5H, cap8H), protein A (spa), haemolysins (hla, hlb, hld, hlgAC), leukocidins (lukD, lukM, lukF-PV, lukS-PV) and enterotoxins (sea, seb, sec4, sed, see, seg, seh, sei, sej).…”

“…S. aureus is indeed one of the most important contagious mastitis-causing bacterial pathogens worldwide and is responsible for clinical and subclinical as well as chronic and acute mastitis (Radostits et al 2010;Zadoks et al 2011;Keefe 2012). S. aureus produces several putative virulence factors contributing to its pathogenicity: protein adhesins, named BMicrobial Surface Components Recognizing Adhesive Matrix Molecules^(or MSCRAMMs) interacting with host extracellular components; polysaccharide intercellular adhesins involved in the production of biofilm; other surface antigens intervening in the evasion of the innate and acquired immune responses (capsules, protein A) and secreted toxins causing cellular and tissue damages, such as haemolysins, leukocidins and enterotoxins (Foster and Höök 1998;Dinges et al 2000;Srinivasan et al 2006;Nishifuji et al 2008;Ote et al 2011;Gogoi-Tiwari et al 2015).…”