Genotypic characterization of amoeba isolated from Acanthamoeba keratitis in Poland

Derda, Monika; Solarczyk, Piotr; Cholewiński, Marcin; Hadaś, E.

doi:10.1007/s00436-015-4319-0

Cited by 12 publications

(9 citation statements)

References 43 publications

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“…Up to 90% of AK cases have been reported in contact lens wearers and related to improper contact lens hygiene. It is emphasized in the literature, and also confirmed by the experience of the authors of the current study, that AK is diagnosed with increasing frequency, including in Poland, along with the spread of the use of contact lenses [8,15,16,[20][21][22][23][24][25][26][27][28][29]. Important risk factors predisposing to Acanthamoeba infections in humans, apart from wearing contact lenses, are damage to the corneal epithelial cells, eye surgery, and exposure of the eye to water containing amphizoic amoeba trophozoites and/or cysts [15,17,21,28,30].…”

Section: Introductionsupporting

confidence: 71%

Effect of povidone iodine, chlorhexidine digluconate and toyocamycin on amphizoic amoebic strains, infectious agents of <i>Acanthamoeba</i> keratitis – a growing threat to human health worldwide

Padzik

Baltaza

Conn

et al. 2018

Ann Agric Environ Med.

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Introduction. Free-living amoebae, ubiquitous in outer environments, in predisposing circumstances may exist as parasites, infectious agents of Acanthamoeba keratitis. In recent decades, the vision-threatening corneal infection is a growing human health threat worldwide, including Poland. The applied therapy is often ineffective due to diagnostic mistakes, various pathogenicity of Acanthamoeba strains and high resistance of cysts to drugs; many agents with possible anti-amoebic activity are still being tested. In the presented study, selected chemicals are investigated in terms of their in vitro effect on corneal and environmental Acanthamoeba strains. Materials and method. Samples of a corneal isolate from a patient with severe Acanthamoeba keratitis,of assessed on the basis of genotype associations of 18S rRNA and the type strain, Acanthamoeba castellanii Neff cultivated in bacteria-free condition, were exposed to povidone iodine, chlorhexidine digluconate or toyocamycin. In vitro population dynamics of the strains were monitored and compared to those of control cultures. Results. All chemicals showed anti-amoebic effects with different degrees of effectiveness. Significant differences were observed in the in vitro population dynamics, and the morpho-physiological status of A. castellanii Neff T4 and corneal strains determined as A. polyphaga T4 genotype, exposed to povidone iodine or toyocamycin, in comparison with chlorhexidine taken as reference. Conclusions. Time-dependent amoebstatic in vitro effects were demonstrated for all agents, in particular, the results of assays with povidone iodine are promising. No significant stimulation of encystation appeared; however, as cysticidal efficacy of chemicals is expected, complementary research is needed on different Acanthamoeba strains with modified agent concentrations and method application.

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Section: Introductionsupporting

confidence: 71%

Effect of povidone iodine, chlorhexidine digluconate and toyocamycin on amphizoic amoebic strains, infectious agents of <i>Acanthamoeba</i> keratitis – a growing threat to human health worldwide

Padzik

Baltaza

Conn

et al. 2018

Ann Agric Environ Med.

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“…The effect of tea tree oil was tested in vitro and in vivo on the Ac55 strain of Acanthamoeba castellanii —isolated from a patient with Acanthamoeba keratitis, genotype T4 GenBank: KP120880, invading both the lungs and the brain of laboratory animals, which is representative for pathogenic strains (Derda et al 2015 ). The amoebae were bred axenically on a liquid medium containing 2% Bacto-Casitone (Difco) and 10% horse plasma.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the effectiveness of tea tree oil in treatment of Acanthamoeba infection

2017

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Eye diseases caused by amoebae from the genus Acanthamoeba are usually chronic and severe, and their treatment is prolonged and not very effective. The difficulties associated with therapy have led to attempts at finding alternative treatment methods. Particularly popular is searching for cures among drugs made of plants. However, no substances with total efficacy in treating Acanthamoeba keratitis have been identified.Results of our semi in vivo studies of tea tree oil simulating eyeball infection demonstrated 100% effectiveness in the case of both trophozoites and cysts of amoebae from the genus Acanthamoeba. The action of tea tree oil indicates that this is the first substance with a potential ability to quickly and effectively remove the amoebae from the eye. Tea tree oil has the ability to penetrate tissues, which allows it to destroy amoebae in both the shallow and deep layers of the cornea. The present research into the use of tea tree oil in the therapy of Acanthamoeba infection is the first study of this type in parasitology. It offers tremendous potential for effective treatment of Acanthamoeba keratitis and other diseases caused by these protozoa.

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“…Acanthamoeba keratitis is characterized by 16 blurred vision, sensitivity to light, conjunctivitis, eye lid swelling, 17 and reddened eye with watery discharge, and severe pain [7]. 18 Approximately 85-88% cases of Acanthamoeba keratitis are 19 associated with the use of contact lens and hence contact lens 20 wearers are at increased risk of this infection [8,9]. Acanthamoeba 21 keratitis is often linked to contact lens disinfectants that fail to 22 effectively decontaminate contact lens.…”

mentioning

confidence: 99%

Targeting cyst wall is an effective strategy in improving the efficacy of marketed contact lens disinfecting solutions against Acanthamoeba castellanii cysts

Abjani

Khan

Yousuf

et al. 2016

Contact Lens and Anterior Eye

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A B S T R A C TAcanthamoeba cysts are highly resistant to contact lens disinfecting solutions. Acanthamoeba cyst wall is partially made of 1,4 b-glucan (i.e., cellulose) and other complex polysaccharides making it a hardy shell that protects the resident amoeba. Here, we hypothesize that targeting the cyst wall structure in addition to antiamoebic compound would improve the efficacy of marketed contact lens disinfecting solutions. Using chlorhexidine as an antiamoebic compound and cellulase enzyme to disrupt cyst wall structure, the findings revealed that combination of both agents abolished viability of Acanthamoeba castellanii cysts and trophozoites. When tested alone, none of the agents nor contact lens disinfecting solutions completely destroyed A. castellanii cysts and trophozoites. The absence of cyst wall-degrading enzymes in marketed contact lens disinfecting solutions render them ineffective against Acanthamoeba cysts. It is concluded that the addition of cyst wall degrading molecules in contact lens disinfecting solutions will enhance their efficacy in decreasing the incidence of Acanthamoeba effectively.

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Genotypic characterization of amoeba isolated from Acanthamoeba keratitis in Poland

Cited by 12 publications

References 43 publications

Effect of povidone iodine, chlorhexidine digluconate and toyocamycin on amphizoic amoebic strains, infectious agents of <i>Acanthamoeba</i> keratitis – a growing threat to human health worldwide

Effect of povidone iodine, chlorhexidine digluconate and toyocamycin on amphizoic amoebic strains, infectious agents of <i>Acanthamoeba</i> keratitis – a growing threat to human health worldwide

Evaluation of the effectiveness of tea tree oil in treatment of Acanthamoeba infection

Targeting cyst wall is an effective strategy in improving the efficacy of marketed contact lens disinfecting solutions against Acanthamoeba castellanii cysts

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