Genotypic characterization of dengue virus strains circulating during 2007-2009 in New Delhi

Chakravarti, Anita; Chauhan, Mohit; Kumar, Suman; Ashraf, Anzar

doi:10.1007/s00705-012-1522-5

Cited by 18 publications

(17 citation statements)

References 34 publications

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“…We identified 2 patients with meningitis: 1 was infected with 2 DENV serotypes and the other with 3 DENV serotypes. We also report the circulation of the DENV-2 genotype Asian II in Brazil, where the DENV-2 genotype American/Asian has been the most prevalent genotype since 1990 ( 13 ). When and where genotype Asian II started to circulate in Brazil is unclear.…”

Section: Discussionmentioning

confidence: 92%

“…The DENV-2 isolates from samples from patients 557 and 571 grouped with isolates of Asian II, which is a genotype that consists of viruses circulating in China, the Philippines, Sri Lanka, Taiwan, and Vietnam ( 13 ). The American/Asian genotype has been the only genotype previously identified in Brazil, although some variations have occurred within this genotype ( 12 ) (Figure, panel B).…”

Section: The Studymentioning

confidence: 99%

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Meningitis Associated with Simultaneous Infection by Multiple Dengue Virus Serotypes in Children, Brazil

Marinho¹,

Oliveira²,

Candiani³

et al. 2017

Emerg. Infect. Dis.

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Section: Discussionmentioning

confidence: 92%

Section: The Studymentioning

confidence: 99%

Meningitis Associated with Simultaneous Infection by Multiple Dengue Virus Serotypes in Children, Brazil

Marinho¹,

Oliveira²,

Candiani³

et al. 2017

Emerg. Infect. Dis.

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“…18 A postviral encephalomyelitis has also been reported in association with DENV that may rarely cause parkinsonism. 20,21 Prior to 2008, DENV-2 and -3 were predominant, with no DENV-1 genotype V in circulation; in 2009 DENV-2 and -3 were again the predominant circulating strains. To our knowledge, chronic DENV infection of the CNS has not been described.…”

Section: Discussionmentioning

confidence: 99%

“…19 Our patient had traveled repeatedly to regions endemic for DENV, but he had no history of acute encephalitis. 20 Sequence analysis of the virus present in the CNS at autopsy revealed an increase in nonsynonymous mutations in the envelope region as compared to other regions of the viral genome (Supplementary Table 4). Here, we demonstrate signs of DENV in the CNS causing a panencephalitis at time of death and at biopsy 33 months antemortem.…”

Section: Discussionmentioning

confidence: 99%

Chronic Dengue Virus Panencephalitis in a Patient with Progressive Dementia with Extrapyramidal Features

Johnson

Larman

Lee

et al. 2019

Annals of Neurology

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Objective: To determine the underlying etiology in a patient with progressive dementia with extrapyramidal signs and chronic inflammation referred to the National Institutes of Health Undiagnosed Diseases Program. Methods: Extensive investigations included metabolic profile, autoantibody panel, infectious etiologies, genetic screening, whole exome sequencing, and the phage-display assay, VirScan, for viral immune responses. An etiological diagnosis was established postmortem. Results: Using VirScan, enrichment of dengue viral antibodies was detected in cerebrospinal fluid as compared to serum. No virus was detected in serum or cerebrospinal fluid, but postmortem analysis confirmed dengue virus in the brain by immunohistochemistry, in situ hybridization, quantitative polymerase chain reaction, and sequencing. Dengue virus was also detectable by polymerase chain reaction and sequencing from brain biopsy tissue collected 33 months antemortem, confirming a chronic infection despite a robust immune response directed against the virus. Immunoprofiling and whole exome sequencing of the patient did not reveal any immunodeficiency, and sequencing of the virus demonstrated wild-type dengue virus in the central nervous system. Interpretation: Dengue virus is the most common arbovirus worldwide and represents a significant public health concern. Infections with dengue virus are usually self-limiting, and chronic dengue infections have not been previously reported. Our findings suggest that dengue virus infections may persist in the central nervous system causing a panencephalitis and should be considered in patients with progressive dementia with extrapyramidal features in endemic regions or with relevant travel history. Furthermore, this work highlights the utility of comprehensive antibody profiling assays to aid in the diagnosis of encephalitis of unknown etiology.

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“…Vigilance over the years recorded its continued circulation in the following years [2,8]. However, with emergence of DENV-3 in 2003, a downward shift in predominance of DENV-2 occurred, and its spread narrowed to sporadic cases [3][4][5]14]. After being persistent at low key for nearly a decade, it emerged as predominant serotype in 2013 outbreak [6].…”

mentioning

confidence: 99%

Molecular epidemiology and evolutionary analysis of dengue virus type 2, circulating in Delhi, India

et al. 2016

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Dengue virus type 2 (DENV-2) has been associated with severe dengue outbreaks in many countries including India. Its predominance was recorded nearly after a decade in the capital city, Delhi in 2013. The present study characterizes DENV-2 circulated during 2013-2014. Analysis based on envelope (E) gene showed the presence of two clades (I and II) of DENV-2, within the Cosmopolitan genotype. Analysis of time of most recent common ancestor revealed the existence of clade I for more than a decade (95 % HPD 13-16 years) however, clade II showed comparatively recent emergence (95 % HPD 5-13 years). Presence of different clades is of high significance as this may result in increased virus transmission and major outbreaks. Further, the presence of a unique amino acid substitution, Q325H was also observed in an isolate; 14/D2/Del/2013 (KT717981). This substitution falls in immune epitope (epitope id: 150268) and may have important role in host immune response.Keywords Dengue Á Molecular epidemiology Á Envelope Á Evolutionary analysis Dengue fever is a mosquito borne infection caused by four antigenically-related serotypes of dengue virus (DENV-1, 2, 3 and 4). During the past decades dengue fever (DF) emerged as a major public health problem of global scale. Nearly 40 % of the world population is living at the risk of dengue infection. According to World Health Organization (available at http://www.searo.who.int/entity/vector_borne _tropical_diseases/data/data_factsheet/en/) it is the major cause of illness and deaths in tropical and subtropical regions, where Aedes mosquito population is high during rainy season. Dengue virus (DENV) belongs to the family Flaviviridae (genus; Flavivirus). Its genome consists of single stranded, positive sense RNA with approximately 11,000 nucleotides. A single open reading frame consists of three structural (capsid; C, premembrane/membrane; prM/ M and envelope; E) and seven nonstructural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5) flanked by nontranslated regions (5 0 UTR and 3 0 UTR) [1].Dengue is endemic in several parts of India. Many outbreaks have been reported from different parts of the country. During last two decades, Delhi, the national capital has emerged as one of the ''badly hit'' city by dengue. The city witnessed its first major dengue hemorrhagic fever (DHF) outbreak in 1996 [2]. Dengue virus type 2 (DENV-2) was confirmed as main etiological agent for this outbreak. During another outbreak in 2003, co-circulation of DENV-2 and DENV-3 was observed [3]. However, in the following years a downward shift in predominance of DENV-2 was recorded. The outbreaks occurred in 2006 and 2010 were predominated by DENV-3 and DENV-1 respectively [4,5]. Nearly after a decade, DENV-2 reemerged as predominant serotype during the outbreak in 2013 [6]. The emergence of DENV-2 is an alarming signal for public health, as this serotype has been associated with major DF and DHF outbreaks [2,[6][7][8]. Based on genomic Electronic supplementary material The online version of this a...

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Genotypic characterization of dengue virus strains circulating during 2007-2009 in New Delhi

Cited by 18 publications

References 34 publications

Meningitis Associated with Simultaneous Infection by Multiple Dengue Virus Serotypes in Children, Brazil

Meningitis Associated with Simultaneous Infection by Multiple Dengue Virus Serotypes in Children, Brazil

Chronic Dengue Virus Panencephalitis in a Patient with Progressive Dementia with Extrapyramidal Features

Molecular epidemiology and evolutionary analysis of dengue virus type 2, circulating in Delhi, India

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