Genotypic Characterization of Mycoplasma Species Isolated From Turkey Flocks

Abstract

M YCOPLASMOSIS in turkeys is a major egg transmitted disease characterized by infra-orbital sinusitis, reduced hatchability, high embryonic mortality, reduced live body weight. Swollen infra-orbital sinus in turkeys resulting in misshapping, and difficult marketing as well as spread of infection to other poultry species. So, this study aimed to investigate Mycoplasmas involved in turkey sinusitis using molecular technique, their phylogenetic analysis versus GenBank database. Suspected turkey flocks (n=17) were investigated using PCR technique for aspirated fluid from infra-orbital sinus. Seven selected isolates of both identified M. gallisepticum (MG), and M. meleagridis (MM) were subjected for sequencing, and phylogenetic analysis. PCR results exhibited single infection of MG and MM in 8, and 3 out of 17 turkey flocks respectively, while mixed infection of both MG and MM were found in 4 flocks. Sequenced MG mgc2 gene of selected seven strains showed similar identity of 93.1-97.6 with each other, 90.4-97.3% with field strains and 84.9-95.9% with reference ones. On the other hand, sequenced 16s rRNA of all isolated seven MM strains revealed complete identity of 100% with each other, 99.7% with MM reference strains, and 83.1-93.1% with other Mycoplasmas spp Both MG, and MM isolates were registered on the GenBank with accession numbers; MW713803, MW713804,

Evaluation of Isolation and Polymerase Chain Reaction in Diagnosis of Mycoplasma Gallisepticum in Broiler Chickens in Kirkuk Governorate, Iraq

“…Reduce of; egg production, hatchability, feed efficiency, and weight. Rise in; mortality, healthcare cost [8,9], bird carcasses with low-quality [10]. Mycoplasma costs the poultry industry a lot of money because it causes a decrease in production by 10-20% and an increase in embryo mortality by 5-10%, add to that control and prevention costs [9,11].…”