Genotypic Comparison of Pasteurella multocida from Healthy Animals at Entry to the Feedlots with That and from Bovine Respiratory Disease-Affected Animals during the Fattening Period

Calderón Bernal, Johan Manuel; Serna, Carlos; García Muñoz, Ángel; Díez Guerrier, Alberto; Domínguez, Lucas; Fernández-Garayzábal, José Francisco; Vela, Ana Isabel; Cid, Dolores

doi:10.3390/ani13172687

Cited by 2 publications

References 35 publications

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Pasteurella multocida from deep nasal swabs and tracheobronchial lavage in bovine calves from Sweden

Myrenås,

Pringle,

Harbom

et al. 2024

Acta Vet Scand

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Background Bovine respiratory disease (BRD) is common in intensively raised cattle and is often treated with antibiotics. For practitioners, knowledge of the bacteria involved in an outbreak and their antibiotic susceptibility is warranted. To this end, samples from the upper or lower respiratory tract of calves can be submitted for bacteriological culture and susceptibility testing of relevant isolates. However, it is debated whether isolates from the upper respiratory tract are representative of bacteria causing infections in the lower respiratory tract. In this study, we used MALDI-TOF MS, multilocus sequence typing (MLST) and core-genome multilocus sequence typing (cgMLST) to compare culture results of 219 paired samples (sample pairs) of deep nasal swabs (DNS) and tracheobronchial lavage (TBL). The sample pairs came from 171 calves in 30 calf groups across 25 farms with 48 calves sampled twice. Results The predominant bacterial pathogen was Pasteurella multocida, which was isolated from 37.4% of DNS and 22.4% of TBL. There was no statistically significant difference in isolation frequency of P. multocida between calves considered healthy and those suspected for BRD for DNS (P = 0.778) or TBL (P = 0.410). Among the 49 sample pairs where P. multocida was isolated from TBL, the same species was isolated from DNS in 29 sample pairs (59.2%). Isolates from 28 of these sample pairs were evaluated by MLST, and in 24 pairs (86.0%) P. multocida from DNS and TBL were of the same sequence type (ST). Moreover, cgMLST showed that the genetic distance between isolates within 21 of the 28 sample pairs (75.0%), was less than two alleles, and DNS and TBL isolates were considered identical. In seven sample pairs (25%), the genetic distance was greater, and DNS and TBL isolates were considered nonidentical. Conclusions Pasteurella multocida was readily isolated from DNS and in calves where this species was isolated also from TBL, DNS and TBL isolates were identical in 75% of the sample pairs. This suggests that during an outbreak of BRD, submission of DNS samples from 4 to 6 calves could be a convenient approach for practitioners seeking guidance on P. multocida present in the lower respiratory tract and their antibiotic susceptibility.

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Pasteurella multocida from deep nasal swabs and tracheobronchial lavage in bovine calves from Sweden

Myrenås,

Pringle,

Harbom

et al. 2024

Acta Vet Scand

View full text Add to dashboard Cite

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Pasteurella multocida from deep nasal swabs and tracheobronchial lavage in calves

Myrenås,

Pringle,

Harbom

et al. 2024

Preprint

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Background Bovine respiratory disease (BRD) is common in intensively raised cattle and is often treated with antibiotics. To guide practitioners, knowledge of the bacteria involved in an outbreak and their antibiotic susceptibility is warranted. To this end, samples from the upper or lower respiratory tract of calves can be submitted for bacteriological culture and susceptibility testing of relevant isolates. However, it is debated whether isolates from the upper respiratory tract are representative of bacteria causing infections in the lower tract. In this study, we used MALDI-TOF MS, multilocus sequence typing (MLST) and core-genome multilocus sequence typing (cgMLST) to compare isolates from deep nasal swabs (DNS) and from tracheobronchial lavage (TBL) in 219 calves from 25 farms. Results: The predominant bacterial pathogen in this study was Pasteurella multocida, which was isolated from 37.4% of DNS and 22.4% of TBL. There was no statistically significant difference in isolation frequency between healthy and diseased calves for DNS (p = 0.778) or TBL (p = 0.410). At least one P. multocida isolate was obtained from 83.3% of the calf groups. At the 49 samplings where P. multocida was isolated from TBL, the same species was isolated from DNS at 29 samplings (59.2%). From 28 of these samplings, isolates were evaluated by MLST and at 24 samplings (86.0%) P. multocida from DNS and TBL were of the same sequence type (ST). Moreover, at 21 of the samplings (75.0%), cgMLST showed that the genetic distance within isolate pairs was less than two alleles, and DNS and TBL isolates were considered identical. At seven samplings (25%), the genetic distance within isolate pairs was greater, and DNS and TBL isolates were considered nonidentical. Within farms and groups of calves, P. multocida of several different STs were isolated. Conclusions: Pasteurella multocida was readily isolated from DNS and in calves where this species was isolated also from TBL, isolates from DNS and TBL were identical at 75% of the samplings. This suggests that during an outbreak of BRD, submission of DNS samples from 4–6 calves could be a convenient approach for practitioners seeking guidance on bacterial pathogens present and their antibiotic susceptibility.

show abstract

Genotypic Comparison of Pasteurella multocida from Healthy Animals at Entry to the Feedlots with That and from Bovine Respiratory Disease-Affected Animals during the Fattening Period

Cited by 2 publications

References 35 publications

Pasteurella multocida from deep nasal swabs and tracheobronchial lavage in bovine calves from Sweden

Pasteurella multocida from deep nasal swabs and tracheobronchial lavage in bovine calves from Sweden

Pasteurella multocida from deep nasal swabs and tracheobronchial lavage in calves

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