Genotypic detection of Epstein-Barr Virus in pediatric transplant recipients from India

Janani, M K; Malathi, Jambulingam; Rosmawati, Mohamed; Farouk, Mohammed; Jayavel, Padmapriya; Madhavan, H. N.

doi:10.1007/s13312-015-0750-7

Cited by 5 publications

(4 citation statements)

References 14 publications

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“…According to earlier Indian studies, keratoconjunctivitis patients had a HAdV positive rate ranging from 13.8% to 66.6% [11][12][13][14]. Similar observations were obtained in our study, conducted in Andaman, with a 24% incidence rate.…”

Section: Figure 1: Neighbor-joining Bootstrap Consensus Phylogenetic ...supporting

confidence: 91%

Adenoviral Conjunctivitis in the Andaman Islands: A Clinical and Molecular Epidemiological Study

Beniwal,

Parvez,

Saharan

et al. 2023

Cureus

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Human adenoviruses are common causes of many acute illnesses, and keratoconjunctivitis is one of them. Acute infections, if left untreated, can progress to severity, thus causing morbidities and mortalities. It belongs to the mastadenovirus family and is characterized by seven subgenus, i.e., A-G; among those, Adenovirus D8 is the most common type associated with keratoconjunctivitis. MethodologyA hospital-based study was conducted, and the samples were collected from GB Pant Hospital, Port Blair, Dr Agarwals Eye Hospital, Port Blair, from August 2017 to December 2022. Clinical data and demographic details were followed by conjunctival swab sample collection from suspected keratoconjunctivitis patients. Samples were subjected to molecular screening, and Sanger sequencing was carried out for positive samples. ResultsOut of 506 conjunctival samples, a prevalence of 24.9% (n=126) was observed, and the commonest type circulating among the population of Andaman was Adenovirus D8. The major symptoms associated were eye redness (87.30%, n=110), followed by watering (81.75%, n=103), eye pain (72.22%, n=91), eye itching (61.11%, n=77), and discharge (50%, n=63). ConclusionIn clinical research, ocular infections are one of the underrated fields. However, the study revealed the high prevalence of adenoviral infection among the suspected patients. Thus, there is a need for proper surveillance and timely diagnosis of such infections, as their severity may lead to loss of vision.

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Section: Figure 1: Neighbor-joining Bootstrap Consensus Phylogenetic ...supporting

confidence: 91%

Adenoviral Conjunctivitis in the Andaman Islands: A Clinical and Molecular Epidemiological Study

Beniwal,

Parvez,

Saharan

et al. 2023

Cureus

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“…Viral infections are known to be a major cause of morbidity and mortality in patients undergoing HSCT and Herpes virus are known to be among the most common viral infections in these patients (5). The iatrogenic suppression of T-cell with the immunosuppression of the transplant regimens, allows the proliferation of infected B cells (9). EBV is one of the most important viruses in transplanted patients, and monitoring of EBV DNA in peripheral blood is routinely performed in several transplant centers, since these patients have a higher-risk of developing complications (9–13).…”

Section: Introductionmentioning

confidence: 99%

Association of Epstein‑Barr virus infection with allogeneic hematopoietic stem cell transplantation in patients in Portugal

et al. 2018

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The identification of patients at higher risk of developing Epstein-Barr virus (EBV) infection in hematopoietic stem cell transplants (HSCT) is useful for the prevention of EBV-associated diseases A prospective observational study was developed that included 40 patients (27 male and 13 females, with mean age of 32.2±1.5 years old) undergoing allogeneic-HSCT between January and December 2015. EBV was examined in whole blood samples collected during routine procedures at day (D)+30, D +60, +90, D+120, D+150 and D+180 post-transplant. EBV was detected, at least once during the follow-up period in 70.0% of our patients. Results indicated that patients with unrelated donors had increased risk of developing EBV infection at D+60 and D+150 (OR=3.9, P=0.058; OR=8.0, P=0.043; respectively). Moreover, myeloablative conditioning (OR=4.3, P=0.052), anti-thymocyte globulin use (OR=12.0, P=0.030) and graft-vs.-host disease (OR=6.7, P=0.032) were associated with EBV infection at D+60, D+150 and D+90, respectively. In our series, none of these patients developed post-transplant lymphoproliferative disease. To the best of our knowledge, the present study is the first study to report EBV infection in patients undergoing aHSCT from Portugal. The study revealed that EBV infection is associated with different factors. These findings provide evidence towards the identification of high-risk patients for EBV-infection and associated disease.

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“…In India, several outbreaks of EKC have been observed, with HAdV-8 being the most common causative agent [24], along with others like HAdV-6 and HAdV-2 [25]. In our previous published report based on sequencing the partial region of hexon we found the isolates to be closer to HAdV-54 than HAdV-8 phylogenetically [26].…”

Section: Introductionmentioning

confidence: 55%

Whole genome analysis of Human Mastadenovirus D causing Keratoconjunctivitis in India - A multicentre study

Philomenadin,

Singh,

Shastri

et al. 2024

J Infect Dev Ctries

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Introduction: Human mastadenovirus (HAdV) types 8, 37, 64 have been considered the major contributors in Epidemic keratoconjunctivitis (EKC) epidemics, but recent surveillance data have shown the involvement of emerging recombinants, including HAdV-53, HAdV-54, and HAdV-56. In our initial work, positive samples for adenovirus revealed that our strains were closer to HAdV-54 than HAdV-8. Hence, the current study aimed to use whole genome technology to identify the HAdV strain correctly. Methodology: Oxford Nanopore technique was used, wherein a Targeted sequencing approach using long-range PCR amplification was performed. Primers were designed using HAdV-54 (AB448770.2) and HAdV-8 (AB897885.1) as reference sequences. Amplicons were sequenced on the GridION sequencer. Sequences were annotated using Gatu software, and similarities with standard reference sequence was calculated using Bioedit software. The phylogenetic tree was built after alignment in MEGA v7.0 using Neighbour joining method for each of the genes: Penton, Hexon, and Fiber. The effect of novel amino acid changes was evaluated using the PROVEAN tool. The Recombination Detection Program (RDP) package Beta 4.1 was used to identify recombinant sequences. Results: Of the five samples sequenced, OL450401, OL540403, and OL540406 showed nucleotide similarity to HAdV-54 in the penton region. Additionally, OL450401 showed a statistically significant recombination event with HAdV-54 as minor and HAdV-8 as major parents. This was further supported by phylogenetic analysis as well. Conclusions: In the present study, we have found evidence of a shift from HAdV-8 towards HAdV-54, thus stressing the need for surveillance of HAdVs and to stay updated on the rise of new recombinants.

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Genotypic detection of Epstein-Barr Virus in pediatric transplant recipients from India

Abstract: EBV infection is the high risk factor for PTLD after liver transplantation. PCR targeting of EBV can be applied to diagnose EBV infections and monitor treatment for EBV in pediatric solid organ transplant recipients.

Cited by 5 publications

References 14 publications

Adenoviral Conjunctivitis in the Andaman Islands: A Clinical and Molecular Epidemiological Study

Adenoviral Conjunctivitis in the Andaman Islands: A Clinical and Molecular Epidemiological Study

Association of Epstein‑Barr virus infection with allogeneic hematopoietic stem cell transplantation in patients in Portugal

Whole genome analysis of Human Mastadenovirus D causing Keratoconjunctivitis in India - A multicentre study

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