Genotypic differences in root and shoot growth of barley (Hordeum vulgare L.) grown under different salinity levels

Abstract

In water-limited areas of Tunisia, more than 30% of subsurface water used for irrigation is saline water, leading to a long-term salinization and degradation processes. To prevent the problems and to minimize the negative impact of using saline water, selection of proper germplasm as well as integrated soil and water management are essential. Understanding the diversity for salt tolerance in barley (Hordeum vulgare L.) genotypes will facilitate their use in genetic improvement. Our objectives were to evaluate, in green, house-salt tolerance for 14 barley cultivars under three different salinity levels: (tap water with an Ec = 0.73 dSm(-1), tap water with 102 mM of NaCl, Ec = 10.76 dSm(-1) and tap water with 151 mM of NaCl, Ec = 15.38 dSm(-1)), and quantify genetic variation based on salt tolerance index, morphological traits, molecular and factorial analysis of correspondence (FAC). The study was conducted in a randomized complete block design arranged as a split plot. The results indicate a great genetic variability to salt tolerance among used barley genotypes. Consequently, it is possible to identify superior cultivars, and evaluate the genotypic performance under salinities conditions. In addition, results obtained were confirmed by clustering made by SSR tool on the base of DNA analysis, which is compatible with the arrangement obtained with statistical method.

Genetic diversity analysis of North Africa’s barley using SSR markers

“…Then it was spread throughout Europe, Asia and North Africa to become perhaps among the most important cereal crop on these regions. This present finding strengthens previous reports on the correlation between eco-geographical distribution and SSR markers [20,7]. It shows also that SSR markers can be used effectively to estimate genetic distances among genotypes and distinguish between naked and hulled barley accessions.…”

“…This was a little bit comparable to that found by Ivandic et al [24], Wang et al [40] and by Bchini et al [7] where their germoplasms were clustered according to the same eco-geographical region. This relative relationship observed between SSR markers and the geographic origin of the North Africa barley accessions may be explained by the long term adaptive conditions under the specific regions or sub-regions of each country.…”

“…In the last few years, studies on agronomic traits [7], isozymes [11] and molecular level ( [8,21,14] showed that North Africa barley accessions has diverse tolerance sources for abiotic stresses and a good nutritional quality [16]. Unfortunately, these studies have been performed but in a fragmentary level.…”

Saline Agriculture in the 21st Century: Using Salt Contaminated Resources to Cope Food Requirements

“…On counterpart, other countries, like Israel, possess crop production practices with unconventional water resources irrigation, and the use of brackish water deserves particular attention. Also, in Tunisia strong investigations were addressed to this thematic [22,23] and one can find agriculture practices based in alternative plant species, most of them are halophytes, which are able to tolerate high temperatures and/or low water availability. Salinity is one of the most widespread soil degradation processes on the Earth.…”

Desalinization of Flooding Periods and Growth of Whole Crop Barley as Early Exposure Area in 'Saemangeum' Newly Reclaimed Land

“…(Kim and Seo, 2006;Seo et al, 2007). 보리는 일반적으로 염을 배제하는 능력이 뛰어나며, 유아로의 Na+의 이동이 낮은 편으로 엽육세포가 Na+의 유해한 작용을 덜 받기 때문에 내염성이 비교적 강한 작물로 분류되어 있으나, 유묘기에는 염 스트레스에 매우 민감하게 반응하는 것으로 알려져 있다 (Bchini et al, 2010;Cho et al, 1998;James et al, 2006;Maas and Hoffmann, 1977;Niazi et al, 1992). `11.02.…”