Genotypic diversity among <i>Shewanella</i> spp. collected from freshwater fish

Paździor, Ewa; Pękala‐Safińska, Agnieszka; Wasyl, Dariusz

doi:10.1111/jfd.12971

Cited by 10 publications

(6 citation statements)

References 28 publications

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“…Shewanella spp. have been isolated from both marine [46], freshwater fish [47] and molluscs [48]. S. putrefaciens, described as a pathogen of marine organisms, is a species commonly isolated from the cloaca of loggerhead sea turtles also in the Mediterranean Sea [9,10,28] as well as reported in this study.…”

Section: Discussionsupporting

confidence: 60%

Antibiotic Resistance of Gram-Negative Bacteria from Wild Captured Loggerhead Sea Turtles

Blasi¹,

Migliore

Mattei³

et al. 2020

Antibiotics

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Sea turtles have been proposed as health indicators of marine habitats and carriers of antibiotic-resistant bacterial strains, for their longevity and migratory lifestyle. Up to now, a few studies evaluated the antibacterial resistant flora of Mediterranean loggerhead sea turtles (Caretta caretta) and most of them were carried out on stranded or recovered animals. In this study, the isolation and the antibiotic resistance profile of 90 Gram negative bacteria from cloacal swabs of 33 Mediterranean wild captured loggerhead sea turtles are described. Among sea turtles found in their foraging sites, 23 were in good health and 10 needed recovery for different health problems (hereafter named weak). Isolated cloacal bacteria belonged mainly to Enterobacteriaceae (59%), Shewanellaceae (31%) and Vibrionaceae families (5%). Although slight differences in the bacterial composition, healthy and weak sea turtles shared antibiotic-resistant strains. In total, 74 strains were endowed with one or multi resistance (up to five different drugs) phenotypes, mainly towards ampicillin (~70%) or sulfamethoxazole/trimethoprim (more than 30%). Hence, our results confirmed the presence of antibiotic-resistant strains also in healthy marine animals and the role of the loggerhead sea turtles in spreading antibiotic-resistant bacteria.

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Section: Discussionsupporting

confidence: 60%

Antibiotic Resistance of Gram-Negative Bacteria from Wild Captured Loggerhead Sea Turtles

Blasi¹,

Migliore

Mattei³

et al. 2020

Antibiotics

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“…This group of bacteria mainly causes pathological lesions observed on the body surface, where dermatitis, skin lesions, and ulcers penetrating subcutaneous muscles were noted ( 15 , 37 , 39 ). Similar clinical signs manifested in fish as necrotic skin lesions or ulcers were detected in infections caused by the Shewanella putrefaciens group ( 46 , 47 , 48 ). Although bacteria of Pseudomonas spp.…”

Section: Discussionsupporting

confidence: 61%

Microbiological analysis of skin lesions of cod (Gadus morhua) from the southern part of the Baltic Sea

Pękala-Safińska,

Nadolna-Ałtyn,

Różycki

et al. 2024

Journal of Veterinary Research

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Introduction Since the middle of the 1980s, severe skin disorders have been observed in Baltic cod (Gadus morhua) each year. Available data on the spectrum of bacteria isolated from the clinical cases being limited, and evaluation of the microbial background of fish skin lesions being useful, a bacteriological examination has been undertaken. Material and Methods A total of 1,381 cod were caught during two voyages of the Baltica research vessel in the Polish exclusive economic zone of the southern Baltic Sea. After an examination which found lesions in 164 of the fish, a microbiological analysis was performed to isolate bacteria from them. The collected strains were phenotyped and genotyped, and their antimicrobial resistance was analysed by minimum inhibitory concentration (MIC) techniques. Results Bacteriological examinations provided 850 isolates. The dominant microorganisms were mesophilic Aeromonas spp., Pseudomonas spp. and Shewanella baltica. Opportunistic bacteria potentially hazardous to human health were also isolated, e.g. Alcaligenes faecalis, Staphylococcus epidermidis, Stenotrophomonas maltophilia and Vibrio sp. The MIC analysis determined the highest number of bacteria to resist sulphamethoxazole and amoxicillin and clavulanic acid. Conclusion Most of the collected bacteria were opportunistic pathogens for fish, widespread in the aquatic environment, and potentially threatening to humans.

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“…Until recently, Shewanella spp. genotypic studies focused on the analysis of 16S rRNA or DNA gyrase subunit B ( gyrB ) PCR amplicons (Beaz‐Hidalgo et al, 2015; Dailey et al, 2016; Jung‐Schroers et al, 2018; Paździor et al, 2019b; Pękala et al, 2015). Multi‐locus sequence analysis has been also used in the phylogenetic analysis of Shewanella spp.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic analysis of Shewanella spp. isolated from fish

Paździor,

Bomba,

Tłuścik

et al. 2023

Journal of Fish Diseases

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Different species of Shewanella spp. widely inhabit freshwater and marine environments. Some of them are opportunistic fish pathogens. The application of high‐throughput sequencing enabled the characterization and taxonomic reclassification of many Shewanella spp. species. Still, some strains collected from fish need to be better recognized. The aim of the present study was to classify and determine the phylogenetic relationships of Shewanella spp. collected from fish. The complete genomes of 94 strains of Shewanella spp. from different fish species were sequenced using Illumina platform (MiSeq). The 16S rRNA gene, genomic features and whole‐genome relationships of those bacteria were comprehensively analysed in comparison to reference strains. Whole‐genome analysis showed that the tested Shewanella spp. strains were clustered into six groups similar to reference strains of S. xiamenensis, S. oneidensis, S. glacialipiscicola, S. hafniensis, S. baltica and S. oncorhynchi. Our study indicates that the whole‐genome sequence analysis enabled taxonomic classification and assessment of the diversity of the Shewanella spp. strains, as opposed to recently the gold standard method of 16S rRNA amplicon sequencing. The high genetic diversity and low similarity to the reference genome of S. oneidensis indicate that the group of strains may be a subspecies or even new species. Furthermore, we showed that the most frequent Shewanella spp. species occurring in freshwater fish in our study is the recently described species S. oncorhynchi.

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Genotypic diversity among Shewanella spp. collected from freshwater fish

Cited by 10 publications

References 28 publications

Antibiotic Resistance of Gram-Negative Bacteria from Wild Captured Loggerhead Sea Turtles

Antibiotic Resistance of Gram-Negative Bacteria from Wild Captured Loggerhead Sea Turtles

Microbiological analysis of skin lesions of cod (Gadus morhua) from the southern part of the Baltic Sea

Phylogenetic analysis of Shewanella spp. isolated from fish

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