Genotypic diversity and pathogenic potential of<i>Yersinia enterocolitica</i>biotype 2 strains isolated in Brazil

Frazão, Miliane Rodrigues; Falcão, Juliana Pfrimer

doi:10.1111/jam.12751

Cited by 10 publications

(5 citation statements)

References 40 publications

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“…There was a lot of study that had a low or negative incidence of such virulence genes (ail and ystA) in pathogenic strains of Y. enterocolitica isolated from meat and other meat products (Falcão et al, 2006;Kot et al, 2007;Bhagat and Virdi, 2007;Ozdemir and Arslan, 2015). On the other hand, previous reports have a high prevalence of such virulence genes isolated from humans (Zheng et al, 2008;Frazão and Falcão, 2015). Besides, The yadA gene was identified in 2.4% of Y. enterocolitica strains in this research, while Tadesse et al (2013) detected yadA gene in 12.8% of Y. enterocolitica strains isolated from porcine.…”

Section: Discussioncontrasting

confidence: 55%

Virulence genotyping and antimicrobial resistance profiles of Yersinia enterocolitica isolated from meat and meat products in Egypt

2021

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Pathogenic Yersinia enterocolitica (Y. enterocolitica) is one of the food-borne entero-pathogen responsible for yersiniosis in humans. The purpose of this research was to survey the prevalence, virulence-associated genes, and antimicrobial resistance of Y. enterocolitica isolated from meat and meat product samples in Egypt. Forty-one (5.9%) out of 700- samples of chicken meat, beef, ground beef, and sausage were positive Y. enterocolitica with a high prevalence in chicken meat (12%). Five virulence genes (ail, inv, ystA, ystB, and yadA) were characterized among 41 Y. enterocolitica isolates with variable frequencies. Among the strains tested, the ystB gene was detected with a high percentage (78.1%), followed by inv gene (70.7%), ail gene (14.6%), ystA gene (12.2%), and yadA gene (2.4%). A high resistance rate was estimated to amoxicillin-clavulanic acid (100%), followed by cefazolin (95%), ampicillin (65.9%), and doxycycline (51.2%), whilst a high sensitivity rate was observed to gentamicin and ciprofloxacin (97.6% each). Interestingly, the multidrug resistance was specified in the 70.7% of strains and showing 13 resistance patterns. Based on nucleotide sequence analysis of the 16s rRNA gene, the phylogenetic tree showed the genetic relatedness amongst Y. enterocolitica isolates. These findings highlighted the emergence of virulent and multidrug-resistant pathogenic Y. entrocolitica in retailed meat and meat products in Egypt.

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Section: Discussioncontrasting

confidence: 55%

Virulence genotyping and antimicrobial resistance profiles of Yersinia enterocolitica isolated from meat and meat products in Egypt

2021

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“…All the B2 O:9 isolates and two of three B4 O:3 isolates analyzed in this work were positive for the inv and yst chromosomal genes. Similarly, Frazão and Falcão, () in Brazil, demonstrated that 40 Y. enterocolitica B2 strains isolated from humans, environment and animals carried the inv and ystA chromosomal genes. The detection of yadA , inv and yst genes is indicative that other virulence plasmid‐ and chromosome‐codified determinants might be expressed (Rusak et al, ).…”

Section: Discussionmentioning

confidence: 84%

“…In epidemiological studies, pulsed field gel electrophoresis (PFGE) has been widely used to establish genomic relations between Y. enterocolitica strains isolated from different sources or regions (Frazão & Falcão, ; Fredriksson‐Ahomaa, Cernela, Hächler, & Stephan, ; Lucero Estrada, Soria, Favier, & Escudero, ).…”

Section: Introductionmentioning

confidence: 99%

Bioserotypes, virulence genes, antimicrobial susceptibility and genomic diversity of Yersinia enterocolitica isolates from Argentina and Chile

Mastrodonato

Favier

Estrada

et al. 2018

Journal of Food Safety

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Yersinia enterocolitica is associated with human clinical manifestations. In this study, the bioserotype distribution, virulence potential, the antimicrobial susceptibility, and the genomic diversity of Y. enterocolitica isolates recovered from Argentina and Chile were assessed. Eight Chilean and 22 Argentine isolates recovered from human (7) and animal feces (3), foods (18) and wastewater (2) were analyzed. They belonged to the bioserotypes B2 O:9 (9), B1A O:5 (8), B1A O:41,42‐41,43 (8), B4 O:3 (3) and B1A O:7,8‐8‐8,19 (2). Autoagglutination (AA), Ca2+‐dependent growth at 37°C and yadA, inv and yst genes were observed in B2 O:9 and B4 O:3 strains. B1A strains exhibited the genotype yadA‐ inv‐ yst+. All strains were resistant to ampicillin, rifampicin and erythromycin. By XbaI‐PFGE, 11 genomic types (GTs) were demonstrated (D.I. 0.90). The characteristics of these Y. enterocolitica isolates highlight the importance to maintain active surveillance of this enteropathogen in both countries. Practical applications The trade exchange among Chile and Argentina includes 12–19% of agricultural goods and foodstuffs with fresh and processed vegetables, seafoods and meat products as the major commodities. Additionally, travel and tourism have increased in recent years with more than 2.5 million passengers moving from Argentina to Chile in 2017. In this context, the control of foodborne pathogens and diseases represents a challenge for both countries. The analysis of bioserotypes, virulence potential, antimicrobial susceptibility, and clonal relatedness of Argentine and Chilean Y. enterocolitica isolates encourages further studies to contribute to the knowledge of the epidemiology of this enteropathogen and the implementation of adequate sanitary measures to prevent Y. enterocolitica illness in both countries.

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“… 4 In Brazil, the isolation and the study of Y. enterocolitica are not common and thus information about the antimicrobial resistance of isolates of this species in this country is scarce. 5 , 6 , 7 , 8 …”

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confidence: 99%

Antimicrobial resistance and plasmid replicons in Yersinia enterocolitica strains isolated in Brazil in 30 years

Frazão

Andrade

Darini

et al. 2017

The Brazilian Journal of Infectious Diseases

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Some studies evaluated the resistance profile of the Y. enterocolitica strains isolated in diverse countries. However, in Brazil the isolation and the study of Y. enterocolitica are not common and therefore information about the antimicrobial resistance profile of this species in this country is scarce. Therefore, the aim of this study was to evaluate the antimicrobial resistance of Y. enterocolitica of biotypes 1A, 2 and 4 isolated from clinical and non-clinical sources between 1979 and 2012, in Brazil. This study showed that some Yersinia enterocolitica of different biotypes remain susceptible to antimicrobials used for gastroenteritis treatment. Moreover, neither acquired resistance genes nor diversity of plasmids replicons were found; however, variation in the in vitro intrinsic resistant pattern was detected, except the non-resistance to cefoxitin in all strains. Notwithstanding, due to epidemiological link between Y. enterocolitica and the pork production chain, monitoring plasmid acquired resistance in Y. enterocolitica could also be considered for antimicrobial resistance control purposes and food safety measures.

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Genotypic diversity and pathogenic potential ofYersinia enterocoliticabiotype 2 strains isolated in Brazil

Cited by 10 publications

References 40 publications

Virulence genotyping and antimicrobial resistance profiles of Yersinia enterocolitica isolated from meat and meat products in Egypt

Virulence genotyping and antimicrobial resistance profiles of Yersinia enterocolitica isolated from meat and meat products in Egypt

Bioserotypes, virulence genes, antimicrobial susceptibility and genomic diversity of Yersinia enterocolitica isolates from Argentina and Chile

Antimicrobial resistance and plasmid replicons in Yersinia enterocolitica strains isolated in Brazil in 30 years

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