Genotypic diversity and phenotypic traits of Streptococcus mutans isolates and their relation to severity of early childhood caries

Valdez, Remberto Marcelo Argandoña; Duque, Cristiane; Caiaffa, Karina Sampaio; Santos, Vanessa Rodrigues dos; Loesch, Maria Luiza de Aguiar; Colombo, Natália Helena; Arthur, Rodrigo Alex; Negrini, Thaís de Cássia; Boriollo, Marcelo Fabiano Gomes; Delbem, Alberto Carlos Botazzo

doi:10.1186/s12903-017-0406-1

Cited by 29 publications

(34 citation statements)

References 32 publications

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“…The medium at 0 (T0) and 60 minutes (T60) was collected and spread with BHI plates. The number of bacteria CFU was calculated after anaerobic incubation of the BHI plate for 48 hours as detailed previously (22,42,43). Bacteria viability at each time point was expressed as the percentage of bacterial growth in relation to T 0 (100%).…”

Section: Growth Condition Of Clinical Strainsmentioning

confidence: 99%

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The enhancing antibiofilm activity of curcumin on Streptococcus mutans strains from severe early childhood caries

Li¹,

Pan²,

Lin³

et al. 2020

Preprint

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Background Streptococcus mutans (S. mutans) is one of the main cariogenic bacteria for caries. It was found that the clinical strains of S. mutans isolated from caries active population have stronger cariogenic ability than the isolates from caries-free (CF) people. Previous studies have found that curcumin can inhibit biofilm formation of S. mutans UA159. The objective of this study is to explore the antibiofilm effect of curcumin on the clinical isolates of S. mutans from severe early childhood caries(SECC).Results The isolates from SECC group had more biomass than CF group (t = 4.296, P < 0.001). The acidogenicity and aciduricity of the strains from two groups showed no significant difference. After treatment with curcumin, the viability of biofilm was reduced to 61.865%±7.108% in SECC and to 84.059%±10.227% in CF group at 24 h (P < 0.05). The net reduction of live bacteria and total bacteria in the SECC group was significantly higher than that of the CF group (live bacteria t = 3.305, P = 0.016; total bacteria t = 2.378, P = 0.045) at 5min. For 24h, the net reduction of live bacteria and total bacteria in the SECC group was significantly higher than that of the CF group (live bacteria t = 3.305, P = 0.016; total bacteria t = 2.378, P = 0.045). The reduction of biofilm thickness reduced significantly in 5min (t = 4.110, P = 0.015) and in 24h (t = 3.453, P = 0.014). Long-term (24h) curcumin treatment inhibited the amount of EPS in SECC group from (25.980±1.156) μm3/μm2 to (20.136±1.042) μm3/μm2, the difference was statistically significant (t = 7.510, P < 0.001). The gene of gtfC, gtfD, ftf, gbpB, fruA and srtA in the CF group and the gtfB, gtfC, gtfD, ftf, gbpB, srtA in SECC group were respectively reduced after 5min curcumin treatment. After 24h treatment, the gtfB, gtfC, gtfD, ftf, gbpB, fruA and srtA in both two groups were downregulation, all the differences were statistically significant.Conclusions Curcumin has antibiofilm activity on clinical strains of S. mutans, especially for those isolated from SECC.

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Section: Growth Condition Of Clinical Strainsmentioning

confidence: 99%

“…It was reported that clinical isolates of S. mutans have different cariogenic properties (16). Strains derived from SECC exhibited cariogenic traits and di cult to remove (22). It is not clear whether curcumin has effect on the S. mutans isolated from SECC.…”

Section: Introductionmentioning

confidence: 99%

The enhancing antibiofilm activity of curcumin on Streptococcus mutans strains from severe early childhood caries

Li¹,

Pan²,

Lin³

et al. 2020

Preprint

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“…In the same research field, Valdez et al, 428 while looking at genotypic diversity and phenotypic traits of S mutans isolates and their relation to severity of early childhood caries, concluded that "although genotypic diversity was similar in children, regardless of caries status, S mutans genotypes from caries-active children were more acid-tolerant and presented higher ability to form biofilm than those isolated from caries-free children. Acid tolerance seems to be the most important S mutans trait related to the pathogenesis of severe early childhood caries.…”

Section: Xylitol and Other Preventive Agentsmentioning

confidence: 99%

Annual review of selected scientific literature: A report of the Committee on Scientific Investigation of the American Academy of Restorative Dentistry

Donovan

Marzola²,

Murphy³

et al. 2018

The Journal of Prosthetic Dentistry

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In 2017, professional literature germane to the clinical practice of prosthodontics was published at an astounding rate. The annual content of over 50 professional journals was searched in its entirety to arrive at this review intended to provide readers with a general clinical update. For convenience, prosthodontics has been divided into 8 subtopics: general prosthodontic considerations, conventional removable complete prosthodontics, conventional removable partial prosthodontics, conventional fixed prosthodontics, general implant prosthodontic considerations, implant removable prosthodontics, implant-fixed prosthodontics, and prosthodontic materials. In addition to the articles selected for review, a sizable volume of excellent general reviews, systematic reviews, meta-analyses, and helpful clinical descriptive materials were also published on topics of prosthodontic interest. Although it would be impractical to provide detailed comments on this voluminous additional material, it is listed here for the reader's convenience: clinical processes, 1-7 anatomy and physiology, 8-20 biomechanics, 21 bruxism, 22-25 conventional complete dentures, 26-28 conventional fixed prosthodontics, 29-33 conventional removable partial prosthodontics, 34-36 diagnosis, 37-43 digital dentistry, 44-47

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“…In addition, S. mutans genotypes from caries-active children were found more acid tolerant and presented higher ability to form biofilms than those isolated from caries-free children. The most important S. mutans trait related to the pathogenesis of sECC is thought to be tolerance to acid [39].…”

Section: Cariogenic Microorganismsmentioning

confidence: 99%

Early Childhood Caries Update

Edem¹

2018

Dental Caries - Diagnosis, Prevention and Management

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Early childhood caries (ECC) is the most common chronic disease among young children who are less than 71 months of age and is currently represented as a public health problem in various countries worldwide. Dental caries continues to be a major health problem in developing nations because of lack of education, awareness, and poor socioeconomic status. It begins with white-spot lesions on upper primary incisors along the margin of the gingiva and leads to complete destruction of the crown. The potential impact of ECC on the general health and development has been widely reported in the literature. The main risk factors in the development of ECC can be categorized as microbiological, dietary, genetic, behavioral, and environmental. Evidences for effective ECC prevention suggest prenatal and immediate postnatal interventions. Population-based early childhood health systems hold great potential to reduce the burden of ECC. This chapter focuses on diagnosis, prevalence, etiology, preventive strategies, and treatment options of ECC.

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Genotypic diversity and phenotypic traits of Streptococcus mutans isolates and their relation to severity of early childhood caries

Cited by 29 publications

References 32 publications

The enhancing antibiofilm activity of curcumin on Streptococcus mutans strains from severe early childhood caries

The enhancing antibiofilm activity of curcumin on Streptococcus mutans strains from severe early childhood caries

Annual review of selected scientific literature: A report of the Committee on Scientific Investigation of the American Academy of Restorative Dentistry

Early Childhood Caries Update

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