Genotypic diversity of Vibrio isolates associated with turbot (Scophthalmus maximus) culture

Montes, M.; Farto, Rosa; Pérez, Marı́a José; Armada, Susana P.; Nieto, T. P.

doi:10.1016/j.resmic.2005.11.008

Cited by 23 publications

(19 citation statements)

References 42 publications

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“…The pattern of Vibrio diversity presented here reflects a snapshot for spring 2010. It is known that the structure of the Vibrio community underlies temporal variation (Montes et al 2006) and shows certain temperature dependence (Pujalte et al 2003). The clear pattern of differentiation among location implies selection for diversification to occur in this host–pathogen system.…”

Section: Discussionmentioning

confidence: 99%

Hosts Are Ahead in a Marine Host-Parasite Coevolutionary Arms Race: Innate Immune System Adaptation in Pipefish Syngnathus Typhle Against Vibrio Phylotypes

et al. 2012

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Microparasites have a higher evolutionary potential than their hosts due to an increased mutation rate and a shorter generation time that usually results in parasites being locally adapted to their sympatric hosts. This pattern may not apply to generalist pathogens as adaptation to sympatric host genotypes is disadvantageous due to a narrowing of the host range, in particular under strong gene flow among host populations. Under this scenario, we predict that the immune defense of hosts reveals adaptation to locally common pathogen phylotypes. This was tested in four host populations of the pipefish Syngnathus typhie and associated bacteria of the genus Vibrio. We investigated the population divergence among host and bacteria populations and verified that gene flow is higher among host populations than among parasite populations. Next, we experimentally assessed the strength of innate immune defense of pipefish hosts using in vitro assays that measured antimicrobial activity of blood plasma against sympatric and allopatric Vibrio phylotypes. Pipefish plasma displays stronger antimicrobial activity against sympatric Vibrio phylotypes compared to allopatric ones. This suggests that host defense is genetically adapted against local bacteria with a broad and unspecialized host spectrum, a situation that is typical for marine systems with weak host population structure.

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Section: Discussionmentioning

confidence: 99%

Hosts Are Ahead in a Marine Host-Parasite Coevolutionary Arms Race: Innate Immune System Adaptation in Pipefish Syngnathus Typhle Against Vibrio Phylotypes

et al. 2012

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“…Thus, colonies of some of the isolates were not larger than 0.2 mm diameter after 3 days of incubation. Some years later V. lentus was isolated from diseased wild octopus ( Octopus vulgaris ) and from turbot ( Scophthalmus maximus ) also in Spain (Farto et al, 2003; Montes et al, 2003, 2006). In the case of octopus, experimental infections by bath challenge demonstrated that V. lentus was able to reproduce the skin lesions, colonize the internal organs, and induce mortality in healthy octopuses (Farto et al, 2003).…”

Section: New Vibrio Species Associated To Molluscsmentioning

confidence: 99%

New Vibrio species associated to molluscan microbiota: a review

et al. 2014

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The genus Vibrio consists of more than 100 species grouped in 14 clades that are widely distributed in aquatic environments such as estuarine, coastal waters, and sediments. A large number of species of this genus are associated with marine organisms like fish, molluscs and crustaceans, in commensal or pathogenic relations. In the last decade, more than 50 new species have been described in the genus Vibrio, due to the introduction of new molecular techniques in bacterial taxonomy, such as multilocus sequence analysis or fluorescent amplified fragment length polymorphism. On the other hand, the increasing number of environmental studies has contributed to improve the knowledge about the family Vibrionaceae and its phylogeny. Vibrio crassostreae, V. breoganii, V. celticus are some of the new Vibrio species described as forming part of the molluscan microbiota. Some of them have been associated with mortalities of different molluscan species, seriously affecting their culture and causing high losses in hatcheries as well as in natural beds. For other species, ecological importance has been demonstrated being highly abundant in different marine habitats and geographical regions. The present work provides an updated overview of the recently characterized Vibrio species isolated from molluscs. In addition, their pathogenic potential and/or environmental importance is discussed.

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“…Vibrio scophthalmi has been found widely in sea water and in many marine fish, such as common dentex (Dentex dentex L.) [1,2], olive flounder (Paralichthys olivaceus) [3] and turbot (Scophthalmimus maximus) [4]. V. scophthalmi is an opportunistic pathogen of flat fish, primarily infecting olive flounder and turbot [2,5].…”

Section: Introductionmentioning

confidence: 99%

Modified a Colony Forming Unit Microbial Adherence to Hydrocarbons Assay and Evaluated Cell Surface Hydrophobicity and Biofilm Production of Vibrio scophthalmi

Qiao¹,

Li²,

Xu³

et al. 2012

J Bacteriol Parasitol

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Vibrio scophthalmi has been considered as an opportunistic pathogen of the flat fish. There is little information available on V. scophthalmi adhesion to the host, an important step in the initial infection process. The objectives of this study were to (1) develop a modified Microbial Adherence to Hydrocarbons (MATH) assay using Colony Forming Unit (CFU) method and evaluate the cell surface hydrophobicity of V. scophthalmi, (2) detect slime layer and biofilm production of V. scophthalmi and (3) investigate the motility and antibiotic susceptibility associated with biofilm production of V. scophthalmi. The results showed that cell surface hydrophobicity of V. scophthalmi was moderate by Salt Aggregation Test (SAT) and MATH assay. The fraction partitioned to the hydrocarbon phase (%Adh) was determined significantly higher by modified CFU MATH assay than that by classical spectrophotometer MATH assay and microscope MATH assay. V. scophthalmi produced slime layer and biofilm. The optimal biofilm production conditions for V. scophthalmi were: BHIB media or TSB supplemented with 2% NaCl and 0-0.5% glucose and incubation for 24 h. The level of biofilm production is related with the pathogenicity of V. scophthalmi and antibiotics susceptibility. V. scophthalmi was motile with flagellum-mediated swimming and type IV pilus-mediated twitching, but no swarming.

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Genotypic diversity of Vibrio isolates associated with turbot (Scophthalmus maximus) culture

Cited by 23 publications

References 42 publications

Hosts Are Ahead in a Marine Host-Parasite Coevolutionary Arms Race: Innate Immune System Adaptation in Pipefish Syngnathus Typhle Against Vibrio Phylotypes

Hosts Are Ahead in a Marine Host-Parasite Coevolutionary Arms Race: Innate Immune System Adaptation in Pipefish Syngnathus Typhle Against Vibrio Phylotypes

New Vibrio species associated to molluscan microbiota: a review

Modified a Colony Forming Unit Microbial Adherence to Hydrocarbons Assay and Evaluated Cell Surface Hydrophobicity and Biofilm Production of Vibrio scophthalmi

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