Genotypic heterogeneity among strains of<i>Mycoplasma gallinarum</i>

Dovč, Peter; Benčina, Dušan; Zajc, Irena

doi:10.1080/03079459108418809

Cited by 6 publications

(4 citation statements)

References 13 publications

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“…Kleven, University of Georgia, Athens (S6 208 , A5969, R K781 , F), J.M.Bradbury, University of Liverpool, (S6 JMB ), and L. Stipkovits, Hungarian Academy of Science, Budapest (S6 B , X-95). Genomic DNA from different Mycoplasma gallisepticum strains was isolated according to the method described previously (Dovč et al, 1991).…”

Section: Gallisepticum Strainsmentioning

confidence: 99%

VARIABLE DNA SEQUENCE OF THE pMGA GENE IN Mycoplasma gallisepticum

MILOŠEVIČ BERLIČ,

BENČINA,

DOVČ

1998

aas

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Specific fragments of Mycoplasma gallisepticum genomic DNA, representing different regions of pMGA1.1 and pMGA1.2 genes, were amplified by Polymerase Chain Reaction (PCR). Using sequence analysis of pMGA terminal domains, differences in nucleotide sequences among strains of M. gallisepticum were identified. Restriction analysis revealed polymorphism at the 5? -end of the pMGA coding region. By means of sequence analysis it was established that the most variable part of pMGA sequence was the 5? -end of the pMGA gene, the region coding amino-terminal part of pMGA protein, while the 3? -end of the gene was conserved among various M. gallisepticum strains analysed. In some strains insertions or deletions were found that indicate the diversity of the pMGA gene family and might be related to the variable expression of the pMGA gene family. Changes in nucleotide sequences of the pMGA genes were also found within isogenic lineages. Genotypic variability is also confirmed by results of the study of the gene product expression.

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Section: Gallisepticum Strainsmentioning

confidence: 99%

VARIABLE DNA SEQUENCE OF THE pMGA GENE IN Mycoplasma gallisepticum

MILOŠEVIČ BERLIČ,

BENČINA,

DOVČ

1998

aas

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“…The most commonly used enzymes in this type of studies are EcoRI, HindIII, BamHI, BglII, KpnI, XhoI, PstI and HinfI. In Mycoplasma gallinarum the specific restriction patterns of different strains were used for epizootiological study revealing very subtile differences between related strains (Dovč et al, 1991). Additional resolution can be achieved by using advanced electrophoretic techniques for separation of DNA fragments.…”

Section: Restriction Enzyme Analysis Of Genomic Dnamentioning

confidence: 99%

Molecular Identification and Characterization of Avian Mycoplasmas

DOVČ,

BENČINA

1998

aas

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Serological techniques are recommended procedures for the identification of Mycoplasma species. They enable separation of mycoplasmas at the species level and are widely used for practical laboratory identification of the isolated microorganisms. However, complex growth media are required for the isolation of Mycoplasma species and some strains of pathogenic Mycoplasma species grow very fastidious and can not be cultured. Introduction of DNA based diagnostic methods in mycoplasma research enabled survey of the whole genome in a single assay and reveal also information about non-coding regions of the genome. Restriction enzyme analysis is a powerful method for differentiation of close related strains but requires high quality of DNA which can only be obtained from cultured mycoplasmas. The increasing number of cloned mycoplasma genomic fragments are successfully used for identification and differentiation of mycoplasma strains and species in hybridization studies. The polymerase chain reaction enables identification and characterization of mycoplasmas without culture directly from clinical material. Large genomic sequencing projects produced complete nucleotide sequence from two Mycoplasma species and several more are in progress. This data will open the possibility to understand better the biology of mycoplasmas.

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“…The whole genomic DNA from M. gallisepticum strain $6 was isolated [18] and partially digested with EcoRI and BamHI restriction endonucleases. The obtained genomic fragments were ligated with the mixture of EcoRI, BamHI, and EcoRI/BamHI double digested vector pUC19.…”

Section: Gallisepticum Dna Librarymentioning

confidence: 99%

“…Isolation of M. gallisepticum genomic DNA, enzymatic digestion and agarose gel electrophoresis were described elsewhere [18]. M.…”

Section: Southern Blot Analysis Of M Gallisepticum Strainsmentioning

confidence: 99%

Recombinant DNA probes and polymerase chain reaction for detection ofMycoplasma gallisepticumstrains

Dovč

Benčina

Antes

et al. 1994

FEMS Microbiology Letters

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Two recombinant DNA clones, pMG286.2 and pMG301.1, were isolated from the partial genomic library of Mycoplasma gallisepticum strain S6. Recombinant M. gallisepticum specific fragments were used as probes in Southern hybridisation with 10 M. gallisepticum strains whose DNA was digested by EcoRI, HindIII, BglII, RsaI and BamHI. The 1.5 kb fragment pMG301.1 did not show polymorphism in hybridisation patterns with M. gallisepticum strains, while the 3.5 kb fragment pMG286.2 enabled differentiation of M. gallisepticum strains into clusters. The DNA sequence of pMG301.1 was used to design a pair of 27-mer oligonucleotides flanking a 1.3 kb genomic region. These two primers directed specific in vitro amplification of all M. gallisepticum strains assayed giving an expected 1.3 kb product. Digestion of polymerase chain reaction products by DdeI enabled simple differentiation between clusters of M. gallisepticum strains and may be useful for improved epizootiological studies of M. gallisepticum infections in poultry.

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Genotypic heterogeneity among strains ofMycoplasma gallinarum

Cited by 6 publications

References 13 publications

VARIABLE DNA SEQUENCE OF THE pMGA GENE IN Mycoplasma gallisepticum

VARIABLE DNA SEQUENCE OF THE pMGA GENE IN Mycoplasma gallisepticum

Molecular Identification and Characterization of Avian Mycoplasmas

Recombinant DNA probes and polymerase chain reaction for detection ofMycoplasma gallisepticumstrains

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