Genotypic identification of the bovine leukemia virus

Vafin, R. R.; Хазипов, Н. З.; Shaeva, Aigul Y.; Zakirova, Z. R.; Zainullin, L. I.; Tyulkin, S.V.; Абдулина, И Р; Алимов, А. М.

doi:10.3103/s0891416814040120

Cited by 8 publications

(6 citation statements)

References 5 publications

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“…Analysis of BLV gp51 env gene sequences from different locations throughout the world revealed the presence of different genetic groups that correlated with geographic origin [ 12 , 21 ], and thus, the nucleotide and amino acid sequences composition of gp51 are useful genomic markers of BLV in studies of geographical distribution [ 9 ]. A phylogenetic study of BLV env gene has so far identified at least 10 genotypes in the world [ 5 , 7 , 8 , 14 , 16 , 19 ].…”

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confidence: 99%

Phylogenetic analysis of <i>env</i> gene of bovine leukemia virus strains spread in Miyazaki prefecture, Japan

Marawan

Mekata

Hayashi

et al. 2017

The Journal of Veterinary Medical Science

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To understand how the latest dominant bovine leukemia virus (BLV) strains were introduced and spread in the Miyazaki prefecture, we collected blood samples from 3 geographic areas (north, central and south) and carried out sequence analysis of the BLV env gene. Two genotypes, genotype I, and III, were identified and the majority of the strains belonged to genotype I (71/74). To clarify a route of BLV introduction, we divided the strains into 20 subgenotypes based on their nucleotide sequences and performed phylogenetic analysis. Our study indicated that common BLV strains were comparatively evenly distributed even in the area, where the farmers have not introduced cattle from other areas and the cattle have limited exposure to BLV infection in grazing fields.

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confidence: 99%

Phylogenetic analysis of <i>env</i> gene of bovine leukemia virus strains spread in Miyazaki prefecture, Japan

Marawan

Mekata

Hayashi

et al. 2017

The Journal of Veterinary Medical Science

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“…When looking at the geographical context of these genotypes, it is clear that their dispersal is specifically associated with the northern and southern part of the Asian continent. While in the northern part the genotypes G4, G7 and G8 were exclusively identified in countries like Mongolia, Russia and Kazakhstan [26,[45][46][47], in the…”

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confidence: 99%

Molecular Characterization of the env Gene of Bovine Leukemia Virus in Cattle from Pakistan with NGS-Based Evidence of Virus Heterogeneity

et al. 2021

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Characterization of the global genetic diversity of the bovine leukemia virus (BLV) is an ongoing international research effort. Up to now BLV sequences have been classified into eleven distinct genotypes. Although BLV genotyping and molecular analysis of field isolates were reported in many countries, there is no report describing BLV genotypes present in cattle from Pakistan. In this study we examined 27 env gene sequences from BLV-infected cattle coming from four farms located in Khyber Pakhtunkwa, Gilgit Baltisan and Punjab provinces. Phylogenetic analyses revealed the classification of Pakistani sequences into genotypes G1 and G6. The alignment with the FLK-BLV sequence revealed the presence of 45 mutations, namely, seven in genotype G1 and 33 in genotype G6. Five mutations were found in both, G1 and G6 genotypes. Twelve amino acid substitutions were found in the analyzed sequences, of which only one P264S was specific for sequences from Pakistan. Furthermore, a certain degree of nucleotide heterogeneity was identified by NGS. These results highlight the need for further study on the importance of genetic variability of BLV, especially in the context of its pathogenicity and potential effect on serological detection.

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“…Investigations of the last decade have identified 11 BLV genotypes, however, the features of genotype-associated pathogenesis have not been studied [11][12][13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Technology of Bovine Leukemia Virus Genodiagnostics in Cattle, in Produced Raw Materials and Products

Vafin

Gilmanov

Genrikhovich

et al. 2021

Series Chemistry and Technology

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The most important task of the dairy cattle industry is to obtain high quality raw milk. To achieve it, a set of measures is required, including aimed at increasing the biological safety of produced raw materials. The aim of the study was to create a scientific and methodological basis for the Bovine leukemia virus (BLV) gene diagnostics in a combined format of pathogen indication and identification. This required updating the strategy of BLV PCR-RFLP genotyping, consistent with its phylogenetic classification, taking into account the growing knowledge about the genetic diversity of 11 genotypes of the studied viral pathogen. When staging nested PCR, oligonucleotide primers were used, which initiate at the final stage of the reaction the production of a 444 bp env-gene fragment of the pathogen. Five restriction endonucleases were used in PCR-RFLP BLV genotyping of: PvuII, SspI, AsuHPI, HaeIII, and BstX2I. As a result of verification of the developed Bovine leukemia virus method for gene identification with an updated genotyping strategy, a technical result was obtained, expressed in the ability to identify all 11 BLV genotypes discovered to date by interpreting the generated 58 genotype-associated combinations of PCR-RFLP profiles.

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Genotypic identification of the bovine leukemia virus

Cited by 8 publications

References 5 publications

Phylogenetic analysis of <i>env</i> gene of bovine leukemia virus strains spread in Miyazaki prefecture, Japan

Phylogenetic analysis of <i>env</i> gene of bovine leukemia virus strains spread in Miyazaki prefecture, Japan

Molecular Characterization of the env Gene of Bovine Leukemia Virus in Cattle from Pakistan with NGS-Based Evidence of Virus Heterogeneity

Technology of Bovine Leukemia Virus Genodiagnostics in Cattle, in Produced Raw Materials and Products

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