Genotypic selection provides experimental confirmation for an alcohol consumption quantitative trait locus in mouse

McClearn, Gerald E.; Tarantino, Lisa M.; Rodriguez, Lawrence A.; Jones, Byron C.; Blizard, David A.; Plomin, Robert

doi:10.1038/sj.mp.4000320

Cited by 26 publications

(16 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When testing a non-sex-exclusive model, significant associations were found for alcohol acceptance for chromosomes 3, 4, 5, 9, 11, 15, 17, and 18, and for chromosomes 1, 2, 3, 4, 7, 9, 10, 12, 15, and 17 for alcohol preference. These results show congruence with previous work in our laboratory [McClearn et al, 1997;Rodriguez et al, 1995]. When testing for a sex effect, significant associations were detected in chromosomes 1, 2, 3, 5, 6, 7, 9, 12, 15, 16, 17, and 19 for alcohol acceptance and chromosomes 1,3,5,6,7,8,9,10,11,12,13,14,15,16,17,18, and 19 for alcohol preference.…”

Section: Resultssupporting

confidence: 95%

“…This confirmation can be achieved by analyzing intercross groups such as other RI sets, F 2 s ], or backcrosses , congenic strains [Bailey, 1981;Oliverio and Eleftheriou, 1976], or genotypic selection , [Bennett, 1997;McClearn et al, 1997]. This two-stage strategy can also be effective in the reduction of Type II errors by utilizing less stringent alpha levels used for the initial RI screen [Belknap et al, 1996].…”

Section: Errorsmentioning

confidence: 97%

See 1 more Smart Citation

Sex-exclusive quantitative trait loci influences in alcohol-related phenotypes

et al. 1999

View full text Add to dashboard Cite

During the past half century, researchers have identified and examined sex differences in alcohol-related phenotypes, focusing more recently on understanding of the mechanisms underlying these differences. In general, the genetic contributions influencing these differences are not consistent with an interpretation of sex linkage and must, therefore, reflect some form of sex limitation in which allelic differences at particular autosomal loci have different consequences in males and females. Significant sex differences in measures of alcohol consumption in mice have been demonstrated in previous work in our laboratory. To investigate these differences further, we explore the limiting case of sex-exclusive effects using data from (BXD) recombinant inbred (RI) strains of mice and from an intercross derived from the same progenitors, C57BL/6J (B) and DBA/2J (D). By the use of two statistical approaches (examination of residual scores as a sex-exclusive phenotypic value for the RI strains and multivariate regression on sex and genotype in the F(2)) we have identified and confirmed female-exclusive markers for alcohol acceptance on chromosomes 9 and 12 and one marker for alcohol preference on chromosome 2. Am. J. Med. Genet. (Neuropsychiatr. Genet.) 88:647-652, 1999.

show abstract

Section: Resultssupporting

confidence: 95%

Section: Errorsmentioning

confidence: 97%

Sex-exclusive quantitative trait loci influences in alcohol-related phenotypes

et al. 1999

View full text Add to dashboard Cite

show abstract

“…Interestingly, not all the variations in Stx12 expression are accounted for by its locus on chromosome 4. In fact, some of it may be attributed to chromosome 15, which also carries several QTL for AP (McClearn et al 1997;Phillips et al 1994). As seen in our BXD RI line analysis, the expression of Stx12 may be attributable to at least two genomic sites in mice.…”

Section: Discussionmentioning

confidence: 86%

Studies on Syntaxin 12 and Alcohol Preference Involving C57BL/6J and DBA/2J Strains of Mice

2008

View full text Add to dashboard Cite

C57BL/6J and DBA/2J inbred mouse strains have been extensively studied for the genetic dissection of alcohol-related phenotypes. We have previously found Syntaxin 12 to be associated with alcohol preference in C57BL/6J and DBA/2J due to its strain-specific and ethanol responsive expression in the male brain. In the current study, we combined genetic and expression analyses to assess the segregation of Syntaxin 12 c.*1370G>A polymorphism with its strain-specific expression and alcohol preference in an F (2) population (N = 427) derived from C57BL/6J and DBA/2J strains. Syntaxin 12 c.*1370G>A polymorphism was found to segregate with alcohol preference in the B6D2F2 population and a correlation was identified between Syntaxin 12 expression and alcohol preference in the selected B6D2F2 males (r = -0.473, r (2) = 0.22). We followed up our analysis in the BXD RI lines using resources from WebQTL and the Mouse Phenome Database. Our study detected significant associations of Syntaxin 12 molecular variants with its level of expression and alcohol preference in B6D2F2 males. Overall, our findings support a role for Syntaxin 12 as a potential contributor to alcohol preference in mice.

show abstract

“…While candidate gene studies are crucial in testing specific hypotheses regarding the involvement of target genes in the expression of cocaine' behavioral effects, the approach is limited in its capacity to identify novel genes and pathways previously unknown to play a role in the behavioral effects of cocaine. As noted by McClearn et al (1997), the value of QTL studies resides in using heritable differences in inbred strains to identify the genes underlying specific vulnerabilities. The identification of novel candidate genes through QTL and in silico analyses compliment the prevalent targeted gene approach and provide a broader empirically based focus for subsequent targeted gene studies.…”

Section: Candidate Genementioning

confidence: 99%

“…It has been suggested that a multistage approach for linkage reduces the probability of false positives (McClearn et al 1997).…”

Section: Introductionmentioning

confidence: 99%

A verification of previously identified QTLs for cocaine-induced activation using a panel of B6.A chromosome substitution strains (CSS) and A/J x C57Bl/6J F2 mice

Boyle

Gill

2009

Psychopharmacology

View full text Add to dashboard Cite

Collectively, chromosome 5 and 18 QTL have now been replicated in multiple independent crosses derived from the A/J and C57BL/6J progenitors. The use of an in silico analysis highlighted potential candidate genes on chromosomes 5 and 18. The present results complement the targeted gene approach currently prevalent in the study of cocaine and provide a broader empirically based focus for subsequent candidate gene studies.

show abstract

Genotypic selection provides experimental confirmation for an alcohol consumption quantitative trait locus in mouse

Cited by 26 publications

References 2 publications

Sex-exclusive quantitative trait loci influences in alcohol-related phenotypes

Sex-exclusive quantitative trait loci influences in alcohol-related phenotypes

Studies on Syntaxin 12 and Alcohol Preference Involving C57BL/6J and DBA/2J Strains of Mice

A verification of previously identified QTLs for cocaine-induced activation using a panel of B6.A chromosome substitution strains (CSS) and A/J x C57Bl/6J F2 mice

Contact Info

Product

Resources

About