Genotypic variation for 17 isozyme genes among Japanese upland varieties in rice.

Ishikawa, Ryuji; Maeda, Kazuharu; Harada, Takeo; Niizeki, Minoru; Saitô, Kenichi

doi:10.1270/jsbbs1951.42.737

Cited by 17 publications

(20 citation statements)

References 7 publications

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“…These characteristics are quite different from those of the lowland cultivars. Also, in genetic markers including 17 isozyme loci and five RFLP markers, the variation in nuclear genotypes of the Japanese upland cultivars was different from that in the lowland population (Ishikawa et al1991(Ishikawa et al , 1992(Ishikawa et al , 1997. The most-remarkable distinction occurred in the genoype of locus Pgd1.…”

Section: Discussionmentioning

confidence: 99%

“…We have never detected heterozygotes in current genetic resources of the Japanese lowland and upland cultivars in comparison to that of Laotian genetic resources. This is because in Japan there are few indica strains as a pollen donor or a cytoplasmic donor (Ishikawa et al 1991(Ishikawa et al , 1992. In the past, however, several cultivars in the Daitou-mai group, genetically determined as indica cultivars (Ishikawa et al 1992), were cultivated around japonica cultivars.…”

Section: Discussionmentioning

confidence: 99%

“…Their genetic characters have been described in Ishikawa et al (1991Ishikawa et al ( , 1992Ishikawa et al ( , 1997. They were cultivated in lowland or upland fields in Japan.…”

Section: Plant Materialsmentioning

confidence: 99%

“…In Japan, rice plants are cultivated in paddy fields and also in upland fields. We previously reported that the group of upland cultivars showed more polymorphism than the lowland population for isozyme genotypes (Ishikawa et al 1992). Further, upland cultivars are found to carry the upland-specific allele for phosphogluconate dehydrogenase (PGD), which has not been found in lowland cultivars, and no other alleles showed such a difference (Ishikawa et al 1991).…”

Section: Introductionmentioning

confidence: 99%

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Different maternal origins of Japanese lowland and upland rice populations

et al. 2002

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Plastid subtype ID (PS-ID) sequences were determined from sequence data based on CA repeats between genes rpl16 and rpl14 in Japanese lowland and upland cultivars. The PS-ID sequences of Japanese rice cultivars showed that there are different maternal origins between lowland and upland cultivars. One subtype, 6C7A, of PS-ID sequences was predominant in all but one Japanese lowland cultivar and carried a combination of the indica-specific subtype 8C8A and japonica-specific nuclear markers for the isozyme genotype. It is probably a nuclear-cytoplasmic recombinant resulting from natural out-crossing and succeeding self-pollination. The origin of the plastid was re-confirmed by the existence of an indica-specific deletion in the plastid genome. In contrast, the Japanese upland cultivars showed two subtypes, 7C6A and 6C7A, of PS-ID sequences. An upland-specific isozyme allele as a nuclear marker was equally predominant in cultivars carrying each subtype. The existence of these particular upland-specific nuclear and cytoplasmic genotypes suggests that the origin of Japanese upland cultivars is different from that of Japanese lowland cultivars. Cultivars carrying the upland-specific nuclear genotype are common in Southeast Asia, but the combination of the upland-specific nuclear and cytoplasmic genotypes which is the same as the Japanese upland predominant type was found in cultivars only in Taiwan and Indonesia. Japanese upland cultivars are closely related to those cultivars.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Their genetic characters have been described in Ishikawa et al (1991Ishikawa et al ( , 1992Ishikawa et al ( , 1997. They were cultivated in lowland or upland fields in Japan.…”

Section: Plant Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Different maternal origins of Japanese lowland and upland rice populations

et al. 2002

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“…As these differentiations were also detected with enzyme variation by electrophoresis (Chu 1967;Shahi et al 1969;Pai et al 1975), such biochemical markers were preferred to recognize the indica and japonica types because the markers accurately reflected their nuclear compositions (Sano and Morishima 1992). This method was also applied to evaluate the genetic resources of Japanese lowland and upland cultivars (Ishikawa et al 1991(Ishikawa et al , 1992. In our former report (Ishikawa et al 1992), a few indica cultivars could be recognized, based on their isozyme genotype.…”

Section: Introductionmentioning

confidence: 99%

Origin of cytoplasm substituted rice cultivars found in Japan

et al. 2002

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Genetic variation of Japanese rice cultivars were examined. Five of 450 lowland cultivars and another five of 200 upland cultivars were determined as the indica type by using isozyme genotypes and the remainder were of the japonica type. The major characteristics of these indica cultivars, revealed a slender shape of grains, a short apiculus hair length, a positive allele for Ph reaction, and allele-3 for the Pgd1 locus. Three of these indica cultivars showed a non-deletion ORF100, which is essential to the japonica-type plastid. The plastid subtype identity (PS-ID) sequences of these plastids is 6C7A, which is also a japonica-specific repeat unit. Thus, these cultivars were concluded to be naturally generated cytoplasm substituted lines. These plastids were introduced into a indica genetic background from japonica cultivars grown elsewhere. The rest of the indica cultivars revealed a deletion-type ORF100 and plastid subtype 8C8A, both of which are indica-specific. These cultivars carried indica-type allelic constitutions for diagnostic isozyme loci. However, other characters were identical to the cytoplasm-substituted cultivars in Japan. In East and Southeast Asia, cultivars carrying a indica-type nuclear genotype with a japonica-type plastid are restricted to Aus cultivars in the Bengal region. Genetic and historical records suggest that Japanese indica cultivars and the Aus cultivars are closely related. The Aus cultivars acquire necessary genetic constitutions from both indica and japonica cultivars through naturally occurring out-crossing to adapt to a particular cultivation condition in the region. The wide adaptability enabled them to be introduced into a northern region like Japan.

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Comparison of gene expression between upland and lowland rice cultivars under water stress using cDNA microarray

et al. 2007

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To elucidate the differences in the regulation of water stress tolerance between two genotypes of rice, upland-rice (UR, resistant to water stress) and lowland-rice (LR, susceptible to water stress), we constructed subtracted cDNA libraries from polyethyleneglycol (PEG)-treated and non-treated rice seedlings (IRAT109, an upland-rice variety) by suppression subtractive hybridization (SSH), from which about 2,000 recombinant colonies were picked and amplified. Then, a cDNA microarray containing these expressed sequence tags (ESTs) was used to analyze the gene expression profiles in UR and LR in response to PEG treatment. Microarray data revealed that the majority of genes expressed in UR and LR are almost identical and Student's t test showed that 13% of all the ESTs detected in leaves and 7% of that in roots expressed differentially in transcripts abundance between the two genotypes. After sequencing, it was found that 64 and 79 unique ESTs expressed at higher levels in UR and LR, respectively. Many of the ESTs that showed higher expression in UR upon PEG treatment represented genes for transcription factors, genes playing roles in detoxification or protection against oxidative stress, and genes that help in maintaining cell turgor. In contrast, some ESTs that showed higher expression in LR were genes functioning in the degradation of cellular components. Based on data from this study and previous reports, we suggest that overexpression of some genes that expressed at higher level in UR may improve water stress tolerance in LR and other plant species.

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Genotypic variation for 17 isozyme genes among Japanese upland varieties in rice.

Cited by 17 publications

References 7 publications

Different maternal origins of Japanese lowland and upland rice populations

Different maternal origins of Japanese lowland and upland rice populations

Origin of cytoplasm substituted rice cultivars found in Japan

Comparison of gene expression between upland and lowland rice cultivars under water stress using cDNA microarray

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