Genotypic Variation for Micronutrient Content in Traditional and Improved Rice Lines and its Role in Biofortification Programme

Nachimuthu, Vishnu Varthini; Robin, S.; Sudhakar, D.; Rajeswari, S.; Raveendran, M.; Subramanian, K. S.; Tannidi, Shalini; Pandian, Balaji

doi:10.17485/ijst/2014/v7i9.15

Cited by 28 publications

(13 citation statements)

References 17 publications

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“…Therefore, increasing the iron and zinc content and bioavailability in rice grains is an essential target for breeders and offers potential bene ts to a large proportion of the human population. Substantial variations in iron and zinc content of brown rice agreed with Banerjee et al (2010), Nachimuthu et al (2014), andChowdhury et al (2016).…”

Section: Iron and Zn Content Of The Cultivarssupporting

confidence: 79%

Morphological, Biochemical and Molecular Characterization of Indigenous Aromatic Rice of Assam

Bordoloi

Sarma

Barua

et al. 2021

Preprint

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We carried out the morphological, biochemical, and molecular characterization of twenty cultivars of the least studied Joha (aromatic) rice indigenous to Assam. Unweighted Neighbour Joining (UNJ) clustering based on usual Euclidean distances for thirty-seven polymorphic morphological markers grouped the cultivars into three clusters with eight, eleven, and one genotype. The Joha rice cultivars showed highly significant differences for all the quantitative traits except for panicle length. The genotypic and phenotypic coefficients of variability (GCV & PCV) were high for grain yield ha-1 (24.62 & 24.85%) and filled grains panicle-1 (23.69 & 25.02%). All the traits except days to flowering and maturity, flag leaf breadth, and spikelet fertility exhibited high heritability along with high to moderate genetic advance, indicating the predominant role of additive gene action. Mahalanobis D2 analysis revealed three multi-genotypic and four mono-genotypic clusters of the cultivars. The cultivars' average polyunsaturated fatty acids were 37.9% oleic acid, 39.22% linoleic acid, and 0.5% linolenic acid. The fatty acid profile of Local Joha was superior to the other cultivars as it showed a high level of linoleic and linolenic acid and low saturated fatty acid content. Kon Joha 4 and Ronga Joha contained the highest iron (82.88 mg kg-1) and zinc (47.39 mg kg-1), respectively, while protein content of Kon Joha-1 and amylose content of Harinarayan were the highest. Joha (Bihpuria) showed the highest gel consistency of 140.50 mm. Kalijeera, Kunkuni Joha, Kon Joha-5, Manimuni Joha and Kon Joha-2 accorded a strong aroma. PCR amplified 174 alleles with a mean value of 2.64 across the 66 polymorphic SSR markers. PIC values ranged from 0.091 to 0.698, with an average of 0.326. The highly informative (PIC>0.50) markers were RM316, RM283, RM585, RM1388, RM3562, RM171, R1M30, RM118, RM11and RM29 for identification of the twenty aromatic rice cultivars. The UNJ clustering based on Jaccard's coefficients classified the cultivars into three distinct clusters with eight, ten, and two genotypes. Our study revealed the nutritional richness of these specialty Joha rice cultivars and sufficient scope for yield enhancement through their interbreeding to keep quality intact.

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Section: Iron and Zn Content Of The Cultivarssupporting

confidence: 79%

Morphological, Biochemical and Molecular Characterization of Indigenous Aromatic Rice of Assam

Bordoloi

Sarma

Barua

et al. 2021

Preprint

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“…The development of varieties with high zinc would be relevant to alleviate malnutrition, but the lack of information on translocation of nutrients from vegetative tissues to grains is one of the barriers to rice biofortification [ 40 , 66 , 67 ]. Several donors for high zinc in polished rice have been identified through the evaluation of landraces and are being used in development of high zinc breeding lines [ 18 – 21 , 68 ]. In parallel, several studies are being conducted on mechanism of zinc uptake and its translocation into the grain [ 41 , 42 , 69 – 73 ].…”

Section: Discussionmentioning

confidence: 99%

Transporter genes identified in landraces associated with high zinc in polished rice through panicle transcriptome for biofortification

et al. 2018

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Polished rice is poor source of micronutrients, however wide genotypic variability exists for zinc uptake and remobilization and zinc content in brown and polished grains in rice. Two landraces (Chittimutyalu and Kala Jeera Joha) and one popular improved variety (BPT 5204) were grown under zinc sufficient soil and their analyses showed high zinc in straw of improved variety, but high zinc in polished rice in landraces suggesting better translocation ability of zinc into the grain in landraces. Transcriptome analyses of the panicle tissue showed 41182 novel transcripts across three samples. Out of 1011 differentially expressed exclusive transcripts by two landraces, 311 were up regulated and 534 were down regulated. Phosphate transporter-exporter (PHO), proton-coupled peptide transporters (POT) and vacuolar iron transporter (VIT) showed enhanced and significant differential expression in landraces. Out of 24 genes subjected to quantitative real time analyses for confirmation, eight genes showed significant differential expression in landraces. Through mapping, six rice microsatellite markers spanning the genomic regions of six differentially expressed genes were validated for their association with zinc in brown and polished rice using recombinant inbred lines (RIL) of BPT 5204/Chittimutyalu. Thus, this study reports repertoire of genes associated with high zinc in polished rice and a proof concept for deployment of transcriptome information for validation in mapping population and its use in marker assisted selection for biofortification of rice with zinc.

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“…Many lines included in this study are drought tolerant (Nootripathu, Norungan, Vellaikudaivazhai, kallundaikar, kodai, kalinga 3, Kinandang patong, azucena, mattaikar, IR65907-116-1, karuthakar, mattakuruvai, manavari, kallundai, kodaikulathan, kattikar, poongar, thogai samba, vellaikattai, kattukuthalam, kalvalai, chivapu chithiraikar, vellai chithiraikar, kudaivazhai and murugankar). Few lines have significant level of micronutrients in it (Nachimuthu et al 2014 ). This panel has its importance because of its major component as traditional landraces with valuable agronomic traits that are cultivated in the small pockets of Tamil Nadu, India.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Population Structure and Genetic Diversity in Rice Germplasm Using SSR Markers: An Initiative Towards Association Mapping of Agronomic Traits in Oryza Sativa

Nachimuthu

Raveendran

Duraialaguraja

et al. 2015

Rice

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164

105

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BackgroundGenetic diversity is the main source of variability in any crop improvement program. It serves as a reservoir for identifying superior alleles controlling key agronomic and quality traits through allele mining/association mapping. Association mapping based on LD (Linkage dis-equilibrium), non-random associations between causative loci and phenotype in natural population is highly useful in dissecting out genetic basis of complex traits. For any successful association mapping program, understanding the population structure and assessing the kinship relatedness is essential before making correlation between superior alleles and traits. The present study was aimed at evaluating the genetic variation and population structure in a collection of 192 rice germplasm lines including local landraces, improved varieties and exotic lines from diverse origin.ResultsA set of 192 diverse rice germplasm lines were genotyped using 61 genome wide SSR markers to assess the molecular genetic diversity and genetic relatedness. Genotyping of 192 rice lines using 61 SSRs produced a total of 205 alleles with the PIC value of 0.756. Population structure analysis using model based and distance based approaches revealed that the germplasm lines were grouped into two distinct subgroups. AMOVA analysis has explained that 14 % of variation was due to difference between with the remaining 86 % variation may be attributed by difference within groups.ConclusionsBased on these above analysis viz., population structure and genetic relatedness, a core collection of 150 rice germplasm lines were assembled as an association mapping panel for establishing marker trait associations.Electronic supplementary materialThe online version of this article (doi:10.1186/s12284-015-0062-5) contains supplementary material, which is available to authorized users.

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Genotypic Variation for Micronutrient Content in Traditional and Improved Rice Lines and its Role in Biofortification Programme

Cited by 28 publications

References 17 publications

Morphological, Biochemical and Molecular Characterization of Indigenous Aromatic Rice of Assam

Morphological, Biochemical and Molecular Characterization of Indigenous Aromatic Rice of Assam

Transporter genes identified in landraces associated with high zinc in polished rice through panicle transcriptome for biofortification

Analysis of Population Structure and Genetic Diversity in Rice Germplasm Using SSR Markers: An Initiative Towards Association Mapping of Agronomic Traits in Oryza Sativa

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