Genotypic Variation in the Micropropagation of Sri Lankan Exacum Hybrids

Riseman, Andrew; Chennareddy, Siva

doi:10.21273/jashs.129.5.0698

Cited by 7 publications

(3 citation statements)

References 24 publications

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“…Many studies have reported the conventional method for micropropagation of plants belonging to this genus [2,3,[14][15][16][17]. Obtaining the plantlets by the conventional method of propagation involves the usage of nodal regions as explants [11,12] and the duration required to obtain plantlets varies from 90 days to 112 days.…”

Section: Achar and Vinjamurimentioning

confidence: 99%

A Simple and Efficient Micropropagation Protocol for Developing Plantlets of Exacum Bicolor Roxb. – An Endangered, Ornamental, and Antidiabetic Herb

Achar¹,

Vinjamuri²

2021

Asian J Pharm Clin Res

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Objective: Exacum bicolor Roxb. is an endangered medicinal herb due to overexploitation by humans and its inefficient vegetative reproduction. Here, we report an efficient and simple procedure for the regeneration of E. bicolor Roxb. using leaf as an explant. Methods: The optimal concentrations of the hormones needed for callus induction were determined by full factorial method using DOE (Design expert ver. 8.0). The hormones selected based on literature were kinetin, indole acetic acid, and 6-Benzylaminopurine (BAP). Multiple shoot regeneration was carried out in liquid and solid media with the optimal concentrations of the hormones obtained by DOE. Rooting was initiated using Murashige and Skoog media containing naphthalene acetic acid 0.5 mg/l, indole butyric acid (IBA) 1.0 mg/l, and gibberellic acid 3 0.5 mg/l along with 0.2% of activated charcoal. Results: Analysis of full factorial design run showed that BAP in combination with kinetin was effective for the growth of callus and multiple shoot regeneration was higher in liquid media (81.25%). The rate of rooting was observed to be 88.23% and the average number of roots was 0.26. Plantlets with budding apical region and well-established leaves and roots were observed in 30 days. Conclusion: The protocol reported here can be used for effective production of E. bicolor plants in a shorter duration compared to the conventional approach.

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Section: Achar and Vinjamurimentioning

confidence: 99%

A Simple and Efficient Micropropagation Protocol for Developing Plantlets of Exacum Bicolor Roxb. – An Endangered, Ornamental, and Antidiabetic Herb

Achar¹,

Vinjamuri²

2021

Asian J Pharm Clin Res

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“…Shoots were elongated on a secondary medium supplemented with 25 mM 2-isopentenyl adenine (2iP) and were successfully rooted both in vitro and ex vitro. However, responses to in vitro culture can be very genotype-dependent (Gandonou et al, 2005;Riseman and Chennareddy, 2004), and optimization may be needed to compensate for this variation (Debnath, 2003(Debnath, , 2007Gupta et al, 2006;Khanna and Raina, 1998). As part of our overall conservation efforts for georgia plume, an aim of current studies is to define effective protocols for the reintroduction and augmentation of natural populations.…”

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confidence: 99%

In Vitro Shoot Regeneration of Georgia Plume, Elliottia racemosa, from Multiple Genotypes Collected from Wild Populations

Radcliffe¹,

Affolter²,

Wetzstein³

2011

horts

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Georgia plume (Elliottia racemosa, Ericaceae) is a threatened, woody plant endemic to Georgia's Coastal Plain region in the southeastern United States. Populations of the plant have a fragmented distribution within a restricted range and are characterized by low genetic diversity and a lack of sexual recruitment. Georgia plume cannot be effectively propagated using conventional methods. We have developed an in vitro shoot regeneration system that is effective with explants obtained from mature plants in the wild. The objective of this study was to determine the efficacy of using this in vitro protocol to regenerate proliferating shoot cultures from 34 georgia plume genotypes obtained from divergent populations. Young expanding leaves were cultured on Gamborg's media supplemented with 10 μM thidiazuron and 5 μM indole-3-acetic acid. After 8 weeks, tissues were transferred to a shoot elongation medium with 25 μM 2-isopentenyl adenine. Of the 34 genotypes tested, 91% formed shoot primordia and 85% regenerated shoots within 6 months of inoculation. This study verifies that tissue culture can be used to produce adventitious shoots from a wide range of georgia plume genotypes. Within a coordinated conservation program, tissue culture is a feasible system to use for safeguarding and reintroduction of genetically diverse plant material, which may be critical to the survival of this rare species.

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“…In these studies selected somaclones were found to be horticulturally more desirable than the explant source. Riseman and Chennareddy (2004) investigated the effect of growth regulator regimen on Exacum L. in tissue culture and found that different regimes caused genotypic variation in the form of varied ploidy levels. Cammareri et al (2002) detected somaclonal variation (capitulum and disk diameter, ligulate flower number) in Aster cordifolius L. 'White Elegans' regenerated from leaf explants.…”

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confidence: 99%

Somaclonal Variation of Coreopsis Regenerated from Leaf Explants

Trader¹,

Gruszewski²,

Scoggins³

et al. 2006

HortSci

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Coreopsis species (tickseed) can be regenerated from leaf segments allowing the possibility to exploit somaclonal variation as a means to develop novel phenotypes. We used true leaf explants from in vitro seedlings of perennial C. grandiflora (A. Gray) Sherff `Domino' and `Sunray' grown on Murashige and Skoog (MS) basal medium. Two of ten seedlings of `Domino' regenerated freely and others were generally recalcitrant. From these two seedlings, designated E2 and H2, shoots were regenerated and acclimatized to the greenhouse. About 175 plants were established and vernalized from which somaclones were selected based on distinct differences in flower orientation and appearance. The selected somaclones were propagated by division and transplanted to the field in August 2001 in a randomized complete block design with three-plant plots and three replications to determine whether novel characteristics persisted through an additional propagation cycle. In the field, plant height, leaf dimension, flowering, and flower dimensions were scored in June and July 2003. Differences were found between somaclones and similarly propagated E2 and H2 for desirable (more petals per flower, greater flowering, shorter plants), undesirable (less flowering, smaller flowers), and neutral (narrower leaves, taller plants) traits. Open-pollinated (OP) seed was collected and germinated and the seedlings from somaclones that differed significantly from E2 and H2 were evaluated. These maternally selected seedlings were overwintered then planted in the field in May 2004. Most traits that differentiated somaclones from E2 and H2 did not persist in the OP seedling population; however variation that was likely introduced through outcrossing resulted in desirable phenotypes with potential for new cultivar development.

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Genotypic Variation in the Micropropagation of Sri Lankan Exacum Hybrids

Cited by 7 publications

References 24 publications

A Simple and Efficient Micropropagation Protocol for Developing Plantlets of Exacum Bicolor Roxb. – An Endangered, Ornamental, and Antidiabetic Herb

A Simple and Efficient Micropropagation Protocol for Developing Plantlets of Exacum Bicolor Roxb. – An Endangered, Ornamental, and Antidiabetic Herb

In Vitro Shoot Regeneration of Georgia Plume, Elliottia racemosa, from Multiple Genotypes Collected from Wild Populations

Somaclonal Variation of Coreopsis Regenerated from Leaf Explants

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