This study was conducted to evaluate the performance of Italian ryegrass cultivars for salt tolerance under in vitro condition. Italian ryegrass cultivars such as Greenfarm, Florida80, Kowinearly, and Hwasan101 were tested for their tolerance to various sodium chloride levels (0, 50, 150, 250, and 350 mM). The seed germination, growth, and activities of antioxidant enzymes were investigated under salt treatment. Physiological traits such as seed germination percentage, germination period, shoot and root length, and dry weight were suppressed under entire salt stress conditions. The results indicated that the highest germination percentage and shoot and root length were recorded at normal conditions. Increased sodium chloride levels caused a significant reduction in the seed germination and growth rate. Among the four tested cultivars, Italian ryegrass 'Hwasan101' could be considered as salt tolerant owing to its higher germination percentage, better seedling growth and antioxidant activities under salinity stress, whereas Greenfarm cultivar was more sensitive. The selection of Italian ryegrass cultivars for greater tolerance to saline environment would allow greater productivity from large saline lands.