Genotyping analysis of colonizing candidal isolates from very-low-birthweight infants in a neonatal intensive care unit

Huang, Yhu Chering; Su, Lin; Wu, Tsung‐Han; Lin, Tzou Yien

doi:10.1016/j.jhin.2004.07.020

Cited by 26 publications

(19 citation statements)

References 24 publications

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“…Fifteen preterms (71.4%) showed multiple site colonization, which is comparable to the results reported by Huang et al (2004). Resistotyping is a phenotypic method which allows differentiation of isolates from clinical samples, and has been used in clinical epidemiological studies (Nakamura et al, 1998;Prasobh et al, 2009), with results complementary to genotypic methods (Leung et al, 2000).…”

Section: Discussionsupporting

confidence: 65%

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Resistant virulent Candida species colonizing preterm neonates and in vitro promising prospect of chlorhexidine gluconate

Wali¹,

Bassyouni²,

El-Seidi³

et al. 2013

Afr. J. Microbiol. Res.

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The present study aimed to investigate the potential virulence factors and antifungal resistance of 31 Candida albicans and 21 non-albicans Candida isolates colonizing preterm neonates. The study also compared the susceptibility results with the in vitro activity of chlorhexidine in the eradication of Candida colonization. Candida albicans produced significantly more phospholipase and coagulase than non-albicans Candida, whereas proteinase production was higher in non-albicans Candida. Biofilm production was demonstrated in Candida albicans and non-albicans Candida (P = 0.214). None of the planktonic growth of Candida isolates were resistant to either fluconazole or amphotericin B, whereas 40% and 84% Candida isolates grown as biofilm became resistant to fluconazole and amphotericin B, respectively. Both coagulase and phospholipase production strongly correlated with the resistance of sessile Candida isolates to amphotericin B (P < 0.001). Whereas both proteinase and phospholipase correlated with the resistance of in vitro Candida biofilms to fluconazole (P < 0.05 and P = 0.001; respectively). Chlorhexidine was comparable to fluconazole towards planktonic and sessile grown Candida isolates. In conclusion, the study demonstrated an association between certain virulence factors and the development of biofilm drug resistance and highlighted the value of chlorhexidine as a promising prospect in the eradication of Candida colonization.

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Section: Discussionsupporting

confidence: 65%

“…However, they may occasionally acquire a new Candida strain of either the same or different species from environmental surfaces and the hands of healthcare workers (Huang et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Resistant virulent Candida species colonizing preterm neonates and in vitro promising prospect of chlorhexidine gluconate

Wali¹,

Bassyouni²,

El-Seidi³

et al. 2013

Afr. J. Microbiol. Res.

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“…Multiple studies have demonstrated horizontal transmission from environmental sources in outbreak settings, and hands of healthcare workers are commonly implicated [12,13]. Molecular techniques have confirmed identity between strains recovered from patients and strains recovered from colonizing sources [14]. Vertical transmission has been implicated as a source of neonatal colonization with C. albicans , with vaginal delivery as an independent risk factor.…”

Section: Epidemiologymentioning

confidence: 99%

Candida parapsilosisand the neonate: epidemiology, virulence and host defense in a unique patient setting

Chow

Linden

Bliss

2012

Expert Review of Anti-infective Therapy

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Invasive candidiasis is a common problem in premature infants that leads to high morbidity and mortality. Although Candida albicans has historically been the most prominent species involved in these infections and has therefore been the subject of the most study, Candida parapsilosis is increasing in frequency, and neonates are disproportionately affected. This article reviews unique aspects of the epidemiology of this organism as well as strategies for prophylaxis against invasive candidiasis in general. Additionally, important differences between C. parapsilosis and C. albicans are coming to light related to virulence determinants and interactions with components of host immunity. These developments are reviewed while highlighting the significant gaps in our understanding that remain to be elucidated.

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“…Of a variety of DNA fingerprinting methods that have been investigated, pulsed-field gel electrophoresis (PFGE) allows the resolution and reproducible separation of DNA of chromosomal size in Candida yeasts; the results of PFGE have been shown to have high rates of interlaboratory agreement (18), and PFGE has been used to analyze the relatedness of C. glabrata strains (10,23). Recently, the use of multilocus sequence typing (MLST) has been proposed to meet the increasing need for global surveillance and comparison of genotypes in a central database via the Internet.…”

mentioning

confidence: 99%

Assessment of Candida glabrata Strain Relatedness by Pulsed-Field Gel Electrophoresis and Multilocus Sequence Typing

Lin

Chen

et al. 2007

J Clin Microbiol

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In this study, 80 Candida glabrata isolates from intensive care unit and human immunodeficiency virus (HIV)-infected patients were typed by multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), and mating type class determination. Among the 25 patients with multiple isolates, 19 patients (76%) contained multiple isolates exhibiting identical or highly related PFGE and MLST genotypes, which may indicate the maintenance or microvariation of one C. glabrata strain in each patient. However, isolates from six patients (24%) displayed different sequence types, PFGE genotypes, or mating type classes, which may indicate colonization with more than one clone over time or strain replacement. High correlations among PFGE genotypes, sequence types, and mating types were found (P < 0.01). MLST exhibited less discriminatory power than PFGE with BssHII. The genotypes, sequence types, and mating type classes were independent of anatomic sources, drug susceptibility, and HIV infection status.

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Genotyping analysis of colonizing candidal isolates from very-low-birthweight infants in a neonatal intensive care unit

Cited by 26 publications

References 24 publications

Resistant virulent Candida species colonizing preterm neonates and in vitro promising prospect of chlorhexidine gluconate

Resistant virulent Candida species colonizing preterm neonates and in vitro promising prospect of chlorhexidine gluconate

Candida parapsilosisand the neonate: epidemiology, virulence and host defense in a unique patient setting

Assessment of Candida glabrata Strain Relatedness by Pulsed-Field Gel Electrophoresis and Multilocus Sequence Typing

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