Genotyping and antimicrobial susceptibility of Clostridium perfringens isolated from dromedary camels, pastures and herders

Fayez, Mahmoud; Elsohaby, Ibrahim; Al-Marri, Theeb; Zidan, Kamal; Al-Doweriej, Ali; El-Sergany, Elham F.; Elmoslemany, Ahmed

doi:10.1016/j.cimid.2020.101460

Cited by 11 publications

(10 citation statements)

References 32 publications

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“…Multidrug resistance was observed in 92% of the isolates, of which 26% were XDR, and the MAR indices ranged from 0.28 to 0.9 with an average of 0.63. These findings conform with previous studies that showed the wide spread of MDR and XDR C. perfringens isolates due to the uncontrolled use of these drugs as growth promoters in animals and for therapeutic uses in humans and animals, which has caused increased resistance [59,62]. It has been shown that MAR higher than 0.2 could be due to contamination from high-risk sources, such as humans and farm animals frequently exposed to antibiotics, thus resulting in potential risk to consumers [73].…”

Section: Discussionsupporting

confidence: 92%

“…Fewer studies have documented the isolation of C. perfringens from camels; in our study, 18% of diarrheic feces from camels were identified, and all nine isolates were of type A, of which seven (77.8%) and three (33.3%) were positive for cpb 2 and cpe genes, respectively. According to Fayez, Elsohaby [ 62 ], the isolation of C. perfringens from diarrheic calves and adults was 73.7%, and most of the isolates were of type A. Moreover, other studies have reported that C. perfringens type A is the predominant type from camels and other animals [ 63 , 64 ].…”

Section: Discussionmentioning

confidence: 99%

“…C. perfringens isolates in this study showed resistance to penicillin, tetracycline, cefotaxime, cefoxitin, ceftriaxone, clindamycin, and chloramphenicol. This occurred following a study on C. perfringens from camels and herders; the findings indicated less effective antimicrobial activity of penicillin and cephems [ 62 ]. The use of penicillin and tetracycline as growth promotors and for the control of Gram-positive bacterial infections may explain the resistance reported in our study to these drugs [ 69 ].…”

Section: Discussionmentioning

confidence: 99%

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Genetic Relatedness, Antibiotic Resistance, and Effect of Silver Nanoparticle on Biofilm Formation by Clostridium perfringens Isolated from Chickens, Pigeons, Camels, and Human Consumers

Ahmed

Bayomi

Hamed³

et al. 2022

Veterinary Sciences

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In this study, we determined the prevalence and toxin types of antibiotic-resistant Clostridium perfringens in chicken, pigeons, camels, and humans. We investigated the inhibitory effects of AgNPs on biofilm formation ability of the isolates and the genetic relatedness of the isolates from various sources determined using RAPD-PCR. Fifty isolates were identified using PCR, and all the isolates were of type A. The cpe and cpb2 genes were detected in 12% and 56% of the isolates, respectively. The effect of AgNPs on biofilm production of six representative isolates indicated that at the highest concentration of AgNPs (100 µg/mL), the inhibition percentages were 80.8–82.8%. The RAPD-PCR patterns of the 50 C. perfringens isolates from various sources revealed 33 profiles and four clusters, and the discriminatory power of RAPD-PCR was high. Multidrug-resistant C. perfringens isolates are predominant in the study area. The inhibition of biofilm formation by C. perfringens isolates was dose-dependent, and RAPD-PCR is a promising method for studying the genetic relatedness between the isolates from various sources. This is the first report of AgNPs’ anti-biofilm activity against C. perfringens from chickens, pigeons, camels, and humans, to the best of our knowledge.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Genetic Relatedness, Antibiotic Resistance, and Effect of Silver Nanoparticle on Biofilm Formation by Clostridium perfringens Isolated from Chickens, Pigeons, Camels, and Human Consumers

Ahmed

Bayomi

Hamed³

et al. 2022

Veterinary Sciences

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“…Another study in Iran reported that 52% of sheep isolates were cpb2 positive in PCR assay, in which 9 of 13 (69%) and 4 of 13 (31%) isolates were taken from diseased and healthy sheep, respectively (Yadegar et al 2018). Mohiuddin et al (2020), showed the percentage of positive isolates for cpb2 was remarkably higher in the diseased population (64%) of sheep and goats, compared to the healthy population (37%), also Fayez et al (2020) indicate that all C. perfringens isolates were found to be associated with cpb2+ genes, were detected only in diarrheic cases.…”

Section: Discussionmentioning

confidence: 99%

Molecular identification and sequence analysis of Clostridium perfringens virulence genes isolated from sheep and goats

Amini,

Kheirkhah,

Shamsaddini Bafti

et al. 2023

J Hellenic Vet Med Soc

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Clostridium perfringens (C. perfringens) belongs to the family of Clostridiaceae and produces a wide range of toxins (four major and a variety of minor toxins). Some toxins associated with virulence have been shown to participate in the pathogenesis of enteric diseases in sheep, goats, and other animals. The aim of this study was to determine the presence of minor virulence genes and their genetic diversity in the various toxinotypes of C. perfringens isolates. About 84 isolates collected from sheep and goat flocks were examined and sequenced for the presence of minor virulence genes. Results showed that PFO and cpb2 were found in 79 out of 84 (94%), cpe was identified in 29 out of 84 (35%) and the presence of tpeL was confirmed in 28 out of 84 (33%) isolates, while none of the isolates were identified as carrying the netB gene. This study shows that the prevalence of genes varied among various types of C. perfringens isolates and also sheep and goat samples, furthermore these findings change the toxinotypes of isolates based on a modiﬁed scheme of toxinotype, which incorporated CPE and NetB toxins. The results of this study showed that the dominant minor virulence genes were PFO and cpb2 and the occurrence of cpe and tpeL genes was also diverse. DNA sequencing revealed approximately a sequence similarity of 97-100% with the GenBank database and 3 motation was found in sequence analysis.

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“…In another study conducted in sheep meat, C. perfringens type A was not detected, and types B, C, and D were isolated [33]. All types of C. perfringens are commensal in the gastrointestinal tract of animals, therefore, contamination during slaughter and butchering could be the primary source of C. perfringens in meats [34,35].…”

Section: Discussionmentioning

confidence: 99%

Genotyping and Antimicrobial Susceptibility of Clostridium perfringens and Clostridioides difficile in Camel Minced Meat

et al. 2021

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The present study aimed to determine the occurrence, genotypes, and antimicrobial resistance of Clostridium perfringens (C. perfringens) and Clostridioides difficile (C. difficile) in camel minced meat samples collected from small butcher shops and supermarkets in Al-Ahsa Governorate, Saudi Arabia. A total of 100 camel minced meat samples were randomly collected from small butcher’s shops (n = 50) and supermarkets (n = 50) in Al-Ahsa Governorate, Saudi Arabia. C. perfringens and C. difficile were isolated and identified using the VITEK-2 compact system and 16S rRNA gene amplification. Genotypes, toxin genes, and antimicrobial susceptibility of the isolates were determined. Moreover, ELISA was used to detect C. perfringens and C. difficile toxins. C. perfringens and C. difficile were isolated from 14% and 4% of the tested minced meat samples, respectively. Out of the 14 C. perfringens isolates, type A (64.3%), type B (7.1%), type C (21.5%), and type D (7.1%) were detected. However, out of the four C. difficile isolates, three (75%) were type A+B+ and one (25%) was type A−B+. None of the C. perfringens or C. difficile toxins were identified using ELISA. C. perfringens and C. difficile isolates exhibited a high rate of resistance to tetracycline (56% and 75%, respectively). However, all isolates were susceptible to amoxicillin-clavulanate. Multidrug resistance was observed in three (21.4%) C. perfringens and one (25%) C. difficile isolates. In conclusion, camel minced meat was contaminated with C. perfringens and C. difficile, which present a potential risk of food poisoning. The majority of the isolates were resistant to at least one antimicrobial, and some isolates were multidrug-resistant. Therefore, food safety standards and frequent inspections of abattoirs, small butcher shops, and supermarkets should be enforced.

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Genotyping and antimicrobial susceptibility of Clostridium perfringens isolated from dromedary camels, pastures and herders

Cited by 11 publications

References 32 publications

Genetic Relatedness, Antibiotic Resistance, and Effect of Silver Nanoparticle on Biofilm Formation by Clostridium perfringens Isolated from Chickens, Pigeons, Camels, and Human Consumers

Genetic Relatedness, Antibiotic Resistance, and Effect of Silver Nanoparticle on Biofilm Formation by Clostridium perfringens Isolated from Chickens, Pigeons, Camels, and Human Consumers

Molecular identification and sequence analysis of Clostridium perfringens virulence genes isolated from sheep and goats

Genotyping and Antimicrobial Susceptibility of Clostridium perfringens and Clostridioides difficile in Camel Minced Meat

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