Genotyping and determining the distribution of prevalent G and P types of group A bovine rotaviruses between 2010 and 2012 in Iran

Madadgar, Omid; Nazaktabar, Ahmad; Keivanfar, Hadi; Salehi, Taghi Zahraei; Zadeh, Samad Lotfollah

doi:10.1016/j.vetmic.2015.04.024

Cited by 13 publications

(13 citation statements)

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“…Rotavirus A was detected in feces and intestinal contents collected from dairy and beef calves with a frequency of 57%, which was higher than reports from Argentina and Brazil (17–42%) [ 19 , 20 , 21 , 22 ], and other geographic regions (20–49%) [ 7 , 23 , 24 , 25 ]. On the other hand, in Australia, the frequency of RVA detection was 80%, which is higher than the detected in our study [ 6 ].…”

Section: Discussionmentioning

confidence: 55%

Phylogenetic Analyses of Rotavirus A from Cattle in Uruguay Reveal the Circulation of Common and Uncommon Genotypes and Suggest Interspecies Transmission

et al. 2020

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Uruguay is one of the main exporters of beef and dairy products, and cattle production is one of the main economic sectors in this country. Rotavirus A (RVA) is the main pathogen associated with neonatal calf diarrhea (NCD), a syndrome that leads to significant economic losses to the livestock industry. The aims of this study are to determine the frequency of RVA infections, and to analyze the genetic diversity of RVA strains in calves in Uruguay. A total of 833 samples from dairy and beef calves were analyzed through RT-qPCR and sequencing. RVA was detected in 57.0% of the samples. The frequency of detection was significantly higher in dairy (59.5%) than beef (28.4%) calves (p < 0.001), while it did not differ significantly among calves born in herds that were vaccinated (64.0%) or not vaccinated (66.7%) against NCD. The frequency of RVA detection and the viral load were significantly higher in samples from diarrheic (72.1%, 7.99 log10 genome copies/mL of feces) than non-diarrheic (59.9%, 7.35 log10 genome copies/mL of feces) calves (p < 0.005 and p = 0.007, respectively). The observed G-types (VP7) were G6 (77.6%), G10 (20.7%), and G24 (1.7%), while the P-types were P[5] (28.4%), P[11] (70.7%), and P[33] (0.9%). The G-type and P-type combinations were G6P[11] (40.4%), G6P[5] (38.6%), G10P[11] (19.3%), and the uncommon genotype G24P[33] (1.8%). VP6 and NSP1-5 genotyping were performed to better characterize some strains. The phylogenetic analyses suggested interspecies transmission, including transmission between animals and humans.

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Section: Discussionmentioning

confidence: 55%

Phylogenetic Analyses of Rotavirus A from Cattle in Uruguay Reveal the Circulation of Common and Uncommon Genotypes and Suggest Interspecies Transmission

et al. 2020

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“…Additionally, Madadgar et al in a study reported that the RVA prevalence in 11 provinces of Iran was 28.2% using the same method. 25,26 In another study by Nourmohammadzadeh et al, the prevalence of RVA was reported to be 48% in winter and 16% in summer. In other words, RV was found to be present in 34% of cases using electron microscopy.…”

Section: Discussionmentioning

confidence: 94%

“…Earlier studies indicated that G6, G10, P [11], and P [5] are the most abundant G and P genotypes of bovine RVA and that there is no evidence for the existence of G8 and P [1] genotypes in Iran. 25,28,33,34 There are fewer studies about I genotyping compared to the G and P genotypes of bovine RVA in the world. The results of these studies show that I2 is the most common I genotype in bovine RVA and almost all of the major strains of bovine RVA (e.g., UK, NCDV, and B223) possess I2 genotype.…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic Study of VP6 Gene of Bovine Rotavirus A and Molecular Survey of Bovine Rotaviruses B and C, and Human G and P Genotypes of Rotavirus A in Calves in Iran

Nazaktabar

Madadgar

2020

Int J Enteric Pathog

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Background: Rotavirus (RV) is one of the most important causes of diarrhea in the calf and human neonates. Rotaviruses are divided into nine different serogroups, of which group A is more important compared to other groups. Objective: This study was performed because of the lack of information about the importance and prevalence of bovine rotaviruses B (RVB) and C (RVC) and human genotypes of rotavirus A (RVA) in the bovine population in Iran. Phylogenetic analysis of VP6 of bovine RVA was the second aim of the present study. Materials and Methods: A total of 581 stool specimens were collected from diarrheic calves of 14 provinces and were analyzed by reverse transcription polymerase chain reaction (RT-PCR) and 485 of them were investigated by PAGE electrophoresis to determine the frequency of three rotaviruses A (RVA), B (RVB), and C (RVC). The presence of human G and P genotypes in Iranian bovine population was also evaluated using semi-nested multiplex RT-PCR. Results: RVA was detected by RT-PCR (VP6 gene detection) in 16.2% (94/581) and by PAGE in 22.16% (108/485) and no positive cases of RVB and RVC were confirmed by polyacrylamide gel electrophoresis (PAGE). This study showed that non-A RV groups (B and C) have little role in calf diarrhea in Iran. The results of the phylogenetic study of VP6 sequences of rotaviruses A identified in this study showed that they all belonged to genotype I2 and were classified into three different branches. Specimen isolated in Zanjan showed the highest difference (maximum identity of 94%) with other sequences and clustered along with the Japanese strain, R22. Human G and P genotypes were not found in the studied samples. Conclusion: The results showed that non-A rotaviruses and human genotypes of RVA are of little importance in calf rotavirus diarrhea in Iran. Also, there is the first phylogenetic study of rotavirus A VP6 protein in Iran.

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“…The wide host breadth of RVA strains indicated the potential of RVAs for interspecies transmission. Only one G10 strain was identified in the study, this strain is uncommon in human (Do et al, ; Luchs & Timenetsky Mdo, ) but are frequently found in animals, particularly in cattle, pigs and lambs (Ennima et al, ; Komoto et al, ; Madadgar, Nazaktabar, Keivanfar, Zahraei Salehi, & Lotfollah Zadeh, ; Mohamed, Mansour, El‐Araby, Mor, & Goyal, ; Rajendran & Kang, ). G10P[15] is an unusual combination but has previously been reported in a calf in India (Rajendran & Kang, ).…”

Section: Discussionmentioning

confidence: 95%

Molecular characterization of group A rotavirus from rhesus macaques ( Macaca mulatta ) at human–wildlife interfaces in Bangladesh

Islam

Hossain

Haider

et al. 2019

Transbound Emerg Dis

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Group A rotavirus (RVA) is an important cause of diarrhoea in people, especially children, and animals globally. Due to the segmented nature of the RVA genome, animal RVA strains have the potential to adapt to the human host through reassortment with other co‐infecting human viruses. Macaques share food and habitat with people, resulting in close interaction between these two species. This study aimed to detect and characterize RVA in rhesus macaques (Macaca mulatta) in Bangladesh. Faecal samples (N = 454) were collected from apparently healthy rhesus macaques from nine different sites in Bangladesh between February and March 2013. The samples were tested by one‐step, real‐time, reverse transcriptase‐polymerase chain reaction (PCR). Four percent of samples (n = 20; 95% CI 2.7%–6.7%) were positive for RVA. RVA positive samples were further characterized by nucleotide sequence analysis of two structural protein gene fragments, VP4 (P genotype) and VP7 (G genotype). G3, G10, P[3] and P[15] genotypes were identified and were associated as G3P[3], G3P[15] and G10P[15]. The phylogenetic relationship between macaque RVA strains from this study and previously reported human strains indicates possible transmission between humans and macaques in Bangladesh. To our knowledge, this is the first report of detection and characterization of rotaviruses in rhesus macaques in Bangladesh. These data will not only aid in identifying viral sharing between macaques, human and other animals, but will also improve the development of mitigation measures for the prevention of future rotavirus outbreaks.

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Genotyping and determining the distribution of prevalent G and P types of group A bovine rotaviruses between 2010 and 2012 in Iran

Cited by 13 publications

References 39 publications

Phylogenetic Analyses of Rotavirus A from Cattle in Uruguay Reveal the Circulation of Common and Uncommon Genotypes and Suggest Interspecies Transmission

Phylogenetic Analyses of Rotavirus A from Cattle in Uruguay Reveal the Circulation of Common and Uncommon Genotypes and Suggest Interspecies Transmission

Phylogenetic Study of VP6 Gene of Bovine Rotavirus A and Molecular Survey of Bovine Rotaviruses B and C, and Human G and P Genotypes of Rotavirus A in Calves in Iran

Molecular characterization of group A rotavirus from rhesus macaques ( Macaca mulatta ) at human–wildlife interfaces in Bangladesh

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