Genotyping and DNA microarray based characterization of Staphylococcus aureus isolates from rabbit carcasses

Merz, Axel; Stephan, Roger; Johler, Sophia

doi:10.1016/j.meatsci.2015.11.002

Cited by 15 publications

(11 citation statements)

References 22 publications

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“…Strains in CC121 are globally distributed, causing infections both in humans and animals (Argudin et al, 2013;Gomez-Sanz et al, 2013;Merz et al, 2016;Doudoulakakis et al, 2017). Generally, strains in CC121 are MSSA (Kurt et al, 2013;Moreno-Grúa et al, 2018), although one report showed that MRSA had been found in CC121 (Kurt et al, 2013). With the rapid development of rabbit farming in Fujian Province in south-eastern China, operators should be alert to the prevalence of the ST121 strain in rabbit populations, which causes high mortality.…”

Section: Discussionmentioning

confidence: 99%

Characterisation of Staphylococcus aureus strain causing severe respiratory disease in rabbits

et al. 2019

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Staphylococcus aureus is acknowledged as one of the important pathogens isolated from humans and animals. However, the S. aureus causing severe respiratory diseases in rabbits have not been well characterised. A S. aureus named FZHW001, isolated from the lungs of dead rabbits with severe respiratory disease, was characterised by artificial infection of rabbits, detection of virulence factors, multi-locus sequencing typing and antimicrobial susceptibility test. The FZHW001 infected rabbits showed identical respiratory symptoms to those of naturally infected ones, and the isolate could spread through directed contact among rabbits. The isolate was typed into clonal complex 121 and carried 7 of 13 tested virulence factors. Furthermore, the isolate was identified to be methicillin-susceptible S. aureus and was susceptible to 7 of 12 tested antibiotics. This study first describes the characteristics of S. aureus isolated from rabbits causing severe respiratory disease, which will help in further understanding the pathogenic mechanisms of S. aureus in rabbits.

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Section: Discussionmentioning

confidence: 99%

Characterisation of Staphylococcus aureus strain causing severe respiratory disease in rabbits

et al. 2019

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“…The S. aureus isolates used in this study originated from SFP outbreaks, asymptomatic nasal colonization or cases of infections in humans, as well as rabbit carcasses and bovine mastitis milk. Isolates were selected from a large collection of well-characterized S. aureus strains , for which DNA microarray enterotoxin hybridization patterns, spa types, and clonal complexes had been previously determined and published [ 14 , 28 , 29 , 30 , 31 ]. Detailed information on all S. aureus strains used in this study is provided in Table 1 .…”

Section: Methodsmentioning

confidence: 99%

Sequence Variability in Staphylococcal Enterotoxin Genes seb, sec, and sed

Johler

Sihto

Macori

et al. 2016

Toxins

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Ingestion of staphylococcal enterotoxins preformed by Staphylococcus aureus in food leads to staphylococcal food poisoning, the most prevalent foodborne intoxication worldwide. There are five major staphylococcal enterotoxins: SEA, SEB, SEC, SED, and SEE. While variants of these toxins have been described and were linked to specific hosts or levels or enterotoxin production, data on sequence variation is still limited. In this study, we aim to extend the knowledge on promoter and gene variants of the major enterotoxins SEB, SEC, and SED. To this end, we determined seb, sec, and sed promoter and gene sequences of a well-characterized set of enterotoxigenic Staphylococcus aureus strains originating from foodborne outbreaks, human infections, human nasal colonization, rabbits, and cattle. New nucleotide sequence variants were detected for all three enterotoxins and a novel amino acid sequence variant of SED was detected in a strain associated with human nasal colonization. While the seb promoter and gene sequences exhibited a high degree of variability, the sec and sed promoter and gene were more conserved. Interestingly, a truncated variant of sed was detected in all tested sed harboring rabbit strains. The generated data represents a further step towards improved understanding of strain-specific differences in enterotoxin expression and host-specific variation in enterotoxin sequences.

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“…CC97 isolates belonged to either spa-type t1190 or t2802. spa-type t1190 is a rabbit-specific strain since it has been previously found in rabbit meat [36,37] and lesions [38]. As for spa-type t2802, it has been reported mostly associated with bovine livestock but also with humans [39,40].…”

Section: Characterization Of Cc97 Mrsa Isolatesmentioning

confidence: 99%

Livestock-Associated Methicillin-Resistant Staphylococcus aureus (MRSA) in Purulent Subcutaneous Lesions of Farm Rabbits

Silva

Sousa

Gómez

et al. 2020

Foods

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Methicillin-resistant Staphylococcus aureus (MRSA) are one of the main pathogens associated with purulent infections. MRSA clonal complex 97 (CC97) has been identified in a wide diversity of livestock animals. Therefore, we aimed to investigate the antibiotic resistance profiles of MRSA strains isolated from purulent lesions of food-producing rabbits. Samples from purulent lesions of 66 rabbits were collected in a slaughterhouse in Portugal. Samples were seeded onto ORSAB plates with 2 mg/L of oxacillin for MRSA isolation. Susceptibility to antibiotics was tested by the disk diffusion method against 14 antimicrobial agents. The presence of resistance genes, virulence factors and the immune evasion cluster (IEC) system was studied by polymerase chain reaction. All isolates were characterized by multilocus sequence typing (MLST), agr and spa typing. From the 66 samples analyzed, 16 (24.2%) MRSA were detected. All strains were classified as multidrug-resistant as they were resistant to at least three classes of antibiotics. All isolates showed resistance to penicillin, erythromycin and clindamycin. Seven isolates were resistant to gentamicin and harbored the aac(6 )-Ie-aph (2 )-Ia gene. Resistance to tetracycline was detected in 10 isolates harboring the tet(K) gene. The IEC genes were detected in three isolates. MRSA strains belonged to CC97, CC1, CC5, CC15 or CC22. The isolates were assigned to six different spa types. In this study we found a moderate prevalence of multidrug-resistant MRSA strains in food-producing rabbits. This may represent concern for food Foods 2020, 9, 439; doi:10.3390/foods9040439 www.mdpi.com/journal/foods Foods 2020, 9, 439 2 of 10 safety and public health, since cross-contamination may occur, leading to the spread of MRSA and, eventually, the possibility of ingestion of contaminated meat.

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Genotyping and DNA microarray based characterization of Staphylococcus aureus isolates from rabbit carcasses

Cited by 15 publications

References 22 publications

Characterisation of Staphylococcus aureus strain causing severe respiratory disease in rabbits

Characterisation of Staphylococcus aureus strain causing severe respiratory disease in rabbits

Sequence Variability in Staphylococcal Enterotoxin Genes seb, sec, and sed

Livestock-Associated Methicillin-Resistant Staphylococcus aureus (MRSA) in Purulent Subcutaneous Lesions of Farm Rabbits

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