Genotyping and pathotyping of diversified strains of infectious bronchitis viruses circulating in Egypt

Zanaty, Ali; Arafa, Abdel-Satar; Hagag, Naglaa M.; El‐Kady, Magdy F.

doi:10.5501/wjv.v5.i3.125

Cited by 37 publications

(44 citation statements)

References 36 publications

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“…The symptoms observed in the case report are consistent with those described in former outbreaks in Egypt, Turkey, and Poland (Yilmaz et al, 2016;Zanaty et al, 2016;Lisowska et al, 2017). Birds showed respiratory as well as nephropathogenic symptoms.…”

Section: Discussionsupporting

confidence: 89%

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Research Note: First evidence of infectious bronchitis virus Middle-East GI-23 lineage (Var2-like) in Germany

Fischer¹,

Klosterhalfen²,

Kump³

et al. 2020

Poultry Science

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The infectious bronchitis virus Middle-East GI-23 lineage (Var2-like) was observed on a German broiler farm, for the first time. The animals suffered from respiratory and nephropathogenic disease. Gross lesions observed during necropsy included tracheitis, aerosacculitis, and nephritis.Tracheal swabs were tested positive for infectious bronchitis virus Middle-East GI-23 lineage (Var2like) by PCR. Furthermore, sequence analysis of the S1 spike protein showed close relationship to the commercially available vaccine TAbic IBVAR206 and polish isolates.

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Section: Discussionsupporting

confidence: 89%

“…occurred between polish GI-23 IBV strains and German field or vaccine strains (Zanaty et al, 2016). This hypothesis has to be proved in future studies.…”

Section: Discussionmentioning

confidence: 90%

Research Note: First evidence of infectious bronchitis virus Middle-East GI-23 lineage (Var2-like) in Germany

Fischer¹,

Klosterhalfen²,

Kump³

et al. 2020

Poultry Science

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“…The results revealed that these viruses were separated into two distinct groups; variant (variant 2) and classic. Meanwhile Zanaty et al [27] isolated 20 IBV viruses, 16 belonged to variant group and 4 belonged to classic group (Mass-like). The variant group was subdivided into 6 isolates (Egy /Var-1) and 10 isolates (Egy /Var -2).…”

Section: Discussionmentioning

confidence: 99%

Pathological Studies on Infectious Bronchitis Disease in Chickens

El-Fetouh

Mohamed

Refat

et al. 2016

Zagazig Veterinary Journal

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Infectious bronchitis virus has been recognized as highly contagious disease in chickens. In this study, 138 birds from 10 broiler chicken flocks that suffered from respiratory disease were examined to study the pathological lesions associated with IBV infection in naturally infected flocks. Moreover, the infection was confirmed via virus isolation and PCR. Samples were harvested from both Sharkia and Gharbia provinces during one year (October 2014-2015). The clinical examination of the investigated birds showed nasal discharges, gasping and rales. Congested trachea with lumen contained mucus and caseated material at the biforcation of the two main bronchi were characteristic in most of the necropsied birds. The kidneys were enlarged and dark red or pale with the presence of whitish precipitate in the ureters. Four isolates recovered from four different broilers chicken's flocks were further analyzed by S1 glycoprotein gene sequencing. The phylogenetic analysis of the deduced amino acids sequences of the four strains revealed that they were closely related to the IBV Egyptian variant 2 and IS/1494/2006 strains. The histopathological examination of the kidneys showed focal aggregation of lymphocytes in the renal cortex and the medulla. The liver showed multiple focal areas of necrosis which replaced by lymphocyte and heterophils aggregation. The spleen showed thickened capsule with subcapsular hemorrhage and mild to severe focal depletion of lymphocytes. Trachea, lungs, proventriculus, intestine and bursa showed hyperplasia and degenerative changes in their epithelium. Blood vessels showed endotheliosis with perivascular edema and leukocyte infiltration in various organs. Edema was also seen in heart, trachea and lungs. Finally, it could be concluded that infectious bronchitis virus is a major cause and strongly implicated in respiratory and/or renal problems of the Egyptian broiler chicken flocks. Moreover, Egyptian variant 2 still evolving and causing severe damage to renal tissue and various organs.

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“…Each dot represents the mean of the clinical signs scoring of all birds in the group at each time point. 34]. Despite wide spread vaccination with classical and/or variant strain based vaccines in Egypt, IBV outbreaks were frequently reported in vaccinated and non-vaccinated flocks of farms [16], indicating poor protection against the prevalent local variant strain [35].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of bacteriophage efficacy in reducing the impact of single and mixed infections withEscherichia coliand infectious bronchitis in chickens

Tawakol¹,

Nabil²,

Samy³

2019

Infection Ecology & Epidemiology

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Infectious bronchitis virus (IBV) represents a major threat to poultry production worldwide particularly when complicated with bacterial infection. In the present study samples were collected from forty broiler farms with respiratory manifestations to characterize IBV and E. coli. Bacteriophages were isolated and enriched from sampled farms to study its efficacy to control single and mixed infections with E. coli and IBV in vivo. Twelve out of forty farms were positive for IBV. Phylogenetic analysis of partial spike protein revealed that all positive cases clustered within the GI-23 genotype. Eight out of forty farms were positive for E. coli serogroups O26, O78, O86, O114, O119, with O125 found on three farms. Bacteriophage treatment delayed the onset and reduced the severity of clinical signs, and prevented the mortality associated with single and mixed infection with IBV and E. coli. Furthermore, in mixed infections, bacteriophage treatment significantly reduced E. coli as well as IBV shedding. Groups treated with bacteriophages showed a significant reduction of E. coli shedding that gradually decreased over time, in contrast to higher and gradually increasing shedding without bacteriophage treatment. In conclusion, bacteriophage treatment significantly reduced the pathogenicity and shedding of IBVand E. coli in mixed infections. ARTICLE HISTORY

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Genotyping and pathotyping of diversified strains of infectious bronchitis viruses circulating in Egypt

Abstract: These findings indicated that the recent circulating Egyptian IBVs have multiple heterogeneous origins in marked diversifying nature of their spread, with high pathotype in specific pathogen free chicks.

Cited by 37 publications

References 36 publications

Research Note: First evidence of infectious bronchitis virus Middle-East GI-23 lineage (Var2-like) in Germany

Research Note: First evidence of infectious bronchitis virus Middle-East GI-23 lineage (Var2-like) in Germany

Pathological Studies on Infectious Bronchitis Disease in Chickens

Evaluation of bacteriophage efficacy in reducing the impact of single and mixed infections withEscherichia coliand infectious bronchitis in chickens

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