Genotyping, antimicrobial resistance and virulence factor gene profiles of vancomycin resistance Enterococcus faecalis isolated from blood culture

Armin, Shahnaz; Fallah, Fatemeh; Karimi, Abdollah; Rashidan, Marjan; Shirdust, Mehdi; Azimi, Leila

doi:10.1016/j.micpath.2017.05.039

Cited by 21 publications

(19 citation statements)

References 17 publications

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“…In this study, more than 45% of the strains were VRE. Notable resistance to vancomycin among E. faecalis strains was similar to several studies in the country [38,39]. Moreover, increasing VRE prevalence among hospitalized patients was also reported previously from southwestern Iran [40].…”

Section: Discussionsupporting

confidence: 87%

High prevalence of vancomycin and high-level gentamicin resistance in Enterococcus faecalis isolates

Taji

Heidari

Ebrahim‐Saraie

et al. 2018

AMicr

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Multiple drug-resistant enterococci are major cause of healthcare-associated infections due to their antibiotic resistance traits. Among them, Enterococcus faecalis is an important opportunistic pathogen causing various hospital-acquired infections. A total of 53 E. faecalis isolates were obtained from various infections. They were identified by phenotypic and genotypic methods. Determination of antimicrobial resistance patterns was done according to CLSI guidelines. The isolates that were non-susceptible to at least one agent in ≥3 antimicrobial categories were defined as multidrug-resistant (MDR). Detection of antimicrobial resistance genes was performed using standard procedures. According to MDR definition, all of the isolates were MDR (100%). High-level gentamicin resistance was observed among 50.9% of them (MIC ≥ 500 μg/ml). The distributions of aac(6′)-Ie-aph(2′′)-Ia and aph(3′)-IIIa genes were 47.2% and 69.8%, respectively. The aph(2′′)-Ib, aph(2′′)-Ic, aph(2′′)-Id, and ant(4′)-Ia genes were not detected. Vancomycin resistance was found in 45.3% of strains. The vanA gene was detected in 37.7% of isolates, whereas vanB and vanC 1 genes were not observed in any strain. Erythromycin resistance rate was 79.2% and the frequencies of ermB and ermC genes were 88.6% and 69.8%, respectively. The ermA and msrA genes were not present in any of the isolates. Our data indicate a high rate of MDR E. faecalis strains. All of high-level gentamicin-resistant isolates carried at least one of aac(6′)-Ie-aph(2′′)-Ia or aph(3′)-IIIa genes. Distribution of vanA was notable among the isolates. In addition, ermB and ermC were accountable for resistance to erythromycin.

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Section: Discussionsupporting

confidence: 87%

High prevalence of vancomycin and high-level gentamicin resistance in Enterococcus faecalis isolates

Taji

Heidari

Ebrahim‐Saraie

et al. 2018

AMicr

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“…b The data are given as the means of the results from three independent experiment. WT wild-type/parent strain; −, 0.83 ≤ △clpP/WT ratio ≤ 1.2 FsrABDC signal transduction system and GelE are major virulence factors in E. faecalis [41,42]. Thus, it may be that the increased abundances of FsrA and GelE leads enhance virulence of the ΔclpP mutant strain.…”

Section: Discussionmentioning

confidence: 99%

ClpP participates in stress tolerance, biofilm formation, antimicrobial tolerance, and virulence of Enterococcus faecalis

Zheng

Wang

et al. 2020

BMC Microbiol

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Background: ClpP is important for bacterial growth and plays an indispensable role in cellular protein quality control systems by refolding or degrading damaged proteins, but the physiological significance of ClpP in Enterococcus faecalis remains obscure. A clpP deletion mutant (△clpP) was constructed using the E. faecalis OG1RF strain to clarify the effect of ClpP on E. faecalis. The global abundance of proteins was determined by a mass spectrometer with tandem mass tag labeling. Results: The ΔclpP mutant strain showed impaired growth at 20°C or 45°C at 5% NaCl or 2 mM H 2 O 2 . The number of surviving ΔclpP mutants decreased after exposure to the high concentration (50× minimal inhibitory concentration) of linezolid or minocycline for 96 h. The ΔclpP mutant strain also demonstrated decreased biofilm formation but increased virulence in a Galleria mellonella model. The mass spectrometry proteomics data indicated that the abundances of 135 proteins changed (111 increased, 24 decreased) in the ΔclpP mutant strain. Among those, the abundances of stress response or virulence relating proteins: FsrA response regulator, gelatinase GelE, regulatory protein Spx (spxA), heat-inducible transcription repressor HrcA, transcriptional regulator CtsR, ATPase/ chaperone ClpC, acetyl esterase/lipase, and chaperonin GroEL increased in the ΔclpP mutant strain; however, the abundances of ribosomal protein L4/L1 family protein (rplD), ribosomal protein L7/L12 (rplL2), 50S ribosomal protein L13 (rplM), L18 (rplR), L20 (rplT), 30S ribosomal protein S14 (rpsN2) and S18 (rpsR) all decreased. The abundances of biofilm formation-related adapter protein MecA increased, while the abundances of dihydroorotase (pyrC), orotate phosphoribosyltransferase (pyrE), and orotidine-5′-phosphate decarboxylase (pyrF) all decreased in the ΔclpP mutant strain. Conclusion:The present study demonstrates that ClpP participates in stress tolerance, biofilm formation, antimicrobial tolerance, and virulence of E. faecalis.

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“…It is of great importance to determine the clonal relationships among VRE isolates in order to take effective control measures and limiting spread of these microorganisms. For this purpose, numerous molecular typing methods such as randomly amplified polymorphic DNA, pulsed field gel electrophoresis (PFGE), multi locus sequence typing, have been developed (16)(17)(18). However, each of these methods has differences in the reproducibility and discriminatory abilities.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Vancomycin-Resistant Enterococcus faecium Isolates from Patients Admitted to the Intensive Care Unit of Hatay State Hospital

Sakin¹,

Aslantaş²,

Bağcı³

2019

tybd

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Bu çalışmada, Hatay Devlet Hastanesi yoğun bakım ünitesine başvuran hastalardan izole edilen 23 vankomisine dirençli Enterococcus faecium (VREfae) suşunun vankomisine olan direnç mekanizmalarının, virülans genlerinin ve klonal ilişkilerinin belirlenmesi amaçlandı. Gereç ve Yöntem: İzolatların vankomisin minimal inhibitör konsantrasyon (MİK) değerleri E-testi ile antimikrobiyallere olan duyarlılıkları ise disk difüzyon yöntemi ile saptandı. VREfae izolatları arasında klonal ilişki pulsed-field jel elektroforezi (PFGE) ile belirlendi. Vankomisin direnç (vanA, vanB, vanC, vanD, vanE ve vanG) ve virülans genleri (asa1, esp, gelE, hyl ve cylA) polimeraz zincir reaksiyonu (PZR) yöntemi ile araştırıldı. Bulgular: Bütün izolatlar vanA geni taşıdığı tespit edildi ve izolatların 17'si (%73,9) en az bir virülans geni yönünden pozitif bulundu. En yaygın virülans geni olan esp, dokuz izolatta tek başına ve iki izolatta ise hly geni ile birlikte saptandı. hly geni tek başına beş izolatta tespit edilirken ve incelenen diğer virülans genleri (asa1, gelE ve cylA) ise hiçbir izolatta tespit edilmedi. İzolatların tamamı çoğul direnç fenotipi gösterdi. İzolatların vankomisin MİK değerleri E-test ile ≥256 µg/mL olarak bulundu.

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Genotyping, antimicrobial resistance and virulence factor gene profiles of vancomycin resistance Enterococcus faecalis isolated from blood culture

Cited by 21 publications

References 17 publications

High prevalence of vancomycin and high-level gentamicin resistance in Enterococcus faecalis isolates

High prevalence of vancomycin and high-level gentamicin resistance in Enterococcus faecalis isolates

ClpP participates in stress tolerance, biofilm formation, antimicrobial tolerance, and virulence of Enterococcus faecalis

Molecular Characterization of Vancomycin-Resistant Enterococcus faecium Isolates from Patients Admitted to the Intensive Care Unit of Hatay State Hospital

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